| 1. |
- Pacurar, Daniel I., et al.
(författare)
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Agrobacterium tumefaciens : From crown gall tumors to genetic transformation
- 2011
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Ingår i: Physiological and molecular plant pathology. - London : Academic Press. - 0885-5765 .- 1096-1178. ; 76:2, s. 76-81
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Forskningsöversikt (refereegranskat)abstract
- The phytopathogenic bacterium Agrobacterium tumefaciens is the causative agent of crown gall disease on a broad range of plant species. For more than a century this pathogen has fascinated biologists for various reasons. It has been believed that disclosing the mystery of the crown gall tumour disease in plants might help to understand the mechanisms of oncogenesis in general, and eventually use this knowledge to cure cancer in animals and humans. However, as the performer of the only known case of trans-kingdom DNA transfer, this natural genetic engineer is recognised by nowadays biologists particularly for this feature, and Agrobacterium-mediated genetic transformation has become the preferred method to generate transgenic plants. This review briefly highlights the key steps in DNA transfer during natural A. tumefaciens infection and the subsequent development of crown gall disease. It will focus on the adaptation and use of this proof of principle for the purpose of genetic transformation. (C) 2011 Elsevier Ltd. All rights reserved.
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| 2. |
- Skov Kristensen, Peter, et al.
(författare)
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Genetic mapping of the barley lodging resistance locus Erectoides-k
- 2016
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Ingår i: Plant Breeding. - : Wiley. - 0179-9541. ; 135:4, s. 420-428
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Tidskriftsartikel (refereegranskat)abstract
- The barley (Hordeum vulgare L.) mutant erectoides-k.32 (ert-k.32) was isolated in 1947 from an X-ray-mutant population of cultivar ‘Bonus’. The mutant was released as a cultivar in 1958 with the name ‘Pallas’ – one of the first cereal crop cultivars developed from induced mutants. ‘Pallas’ is a semi-dwarf barley cultivar known for its culm stability and resistance to lodging. In total, eight allelic ert-k mutants are known that show different phenotypic strength concerning culm length and spike architecture. They represent alternatives to the widely used, but pleiotropic ‘Green Revolution’ alleles of the Sdw1 (semidwarf1/denso) and Uzu1 (semi-brachytic1) genes in breeding of robust elite barley cultivars. In the present study, we locate Ert-k to a 15.7-cM region in the centromeric region of chromosome 6H. Although the interval is estimated to contain approximately 700 genes, the work provides a solid foundation for the identification of the underlying mutations causing the ert-k lodging-resistant phenotype. In addition, the linked markers could be used to follow the ert-k mutant genotype in marker-assisted selection of new lodging-resistant barley cultivars.
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