| 1. |
- Rydberg Sterner, Therese, et al.
(författare)
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The Gothenburg H70 Birth cohort study 2014-16: design, methods and study population.
- 2019
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Ingår i: European journal of epidemiology. - : Springer Science and Business Media LLC. - 1573-7284 .- 0393-2990. ; 34:2, s. 191-209
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Tidskriftsartikel (refereegranskat)abstract
- To improve health care for older persons, we need to learn more about ageing, e.g. identify protective factors and early markers for diseases. The Gothenburg H70 Birth Cohort Studies (the H70 studies) are multidisciplinary epidemiological studies examining representative birth cohorts of older populations in Gothenburg, Sweden. So far, six birth cohorts of 70-year-olds have been examined over time, and examinations have been virtually identical between studies. This paper describes the study procedures for the baseline examination of the Birth cohort 1944, conducted in 2014-16. In this study, all men and women born 1944 on specific dates, and registered as residents in Gothenburg, were eligible for participation (n=1839). A total of 1203 (response rate 72.2%; 559 men and 644 women; mean age 70.5years) agreed to participate in the study. The study comprised sampling of blood and cerebrospinal fluid, psychiatric, cognitive, and physical health examinations, examinations of genetics and family history, use of medications, social factors, functional ability and disability, physical fitness and activity, body composition, lung function, audiological and ophthalmological examinations, diet, brain imaging, as well as a close informant interview, and qualitative studies. As in previous examinations, data collection serves as a basis for future longitudinal follow-up examinations. The research gained from the H70 studies has clinical relevance in relation to prevention, early diagnosis, clinical course, experience of illness, understanding pathogenesis and prognosis. Results will increase our understanding of ageing and inform service development, which may lead to enhanced quality of care for older persons.
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| 2. |
- Stenhamre, Hanna, et al.
(författare)
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Nanosized fibers' effect on adult human articular chondrocytes behavior.
- 2013
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Ingår i: Materials science & engineering. C, Materials for biological applications. - : Elsevier BV. - 1873-0191 .- 0928-4931. ; 33:3, s. 1539-1545
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Tidskriftsartikel (refereegranskat)abstract
- Tissue engineering with chondrogenic cell based therapies is an expanding field with the intention of treating cartilage defects. It has been suggested that scaffolds used in cartilage tissue engineering influence cellular behavior and thus the long-term clinical outcome. The objective of this study was to assess whether chondrocyte attachment, proliferation and post-expansion re-differentiation could be influenced by the size of the fibers presented to the cells in a scaffold. Polylactic acid (PLA) scaffolds with different fiber morphologies were produced, i.e. microfiber (MS) scaffolds as well as nanofiber-coated microfiber scaffold (NMS). Adult human articular chondrocytes were cultured in the scaffolds in vitro up to 28 days, and the resulting constructs were assessed histologically, immunohistochemically, and biochemically. Attachment of cells and serum proteins to the scaffolds was affected by the architecture. The results point toward nano-patterning onto the microfibers influencing proliferation of the chondrocytes, and the overall 3D environment having a greater influence on the re-differentiation. In the efforts of finding the optimal scaffold for cartilage tissue engineering, studies as the current contribute to the knowledge of how to affect and control chondrocytes behavior.
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| 3. |
- Thorvaldsson, Anna, 1981, et al.
(författare)
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Controlling the Architecture of Nanofiber-Coated Microfibers Using Electrospinning
- 2010
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Ingår i: Journal of Applied Polymer Science. - : Wiley. - 1097-4628 .- 0021-8995. ; 118:1, s. 511-517
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Tidskriftsartikel (refereegranskat)abstract
- This study shows that electrospinning nanofibers onto single microfibers allows for careful tailoring of material properties that may suit a wide variety of applications. The nanofiber-coated microfibers are created by electrospinning nanofibers alongside a microfiber toward a collector that rotates around the microfiber. This force the nanofibers to be collected around the microfiber, creating a hierarchical structure that can be modified at nano scale. In this study, control of nanofiber diameters, nanofiber alignment, and nanofiber loading was evaluated. It was seen that varying polymer concentration affected the nanofiber diameters, collecting the nanofiber-coated microfibers at different speeds changed the degree of alignment of the nanofibers and that changing the polymer feeding rate affected the loading density of the nanofibers collected. The carefully designed nanofiber-coated microfibers have great potential in creation of highly porous materials with tailored properties down to nano scale.
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| 4. |
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| 5. |
- Thorvaldsson, Anna, 1981, et al.
(författare)
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Development of Nanofiber-Reinforced Hydrogel Scaffolds for Nucleus Pulposus Regeneration by a Combination of Electrospinning and Spraying Technique
- 2013
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Ingår i: Journal of Applied Polymer Science. - : Wiley. - 1097-4628 .- 0021-8995. ; 128:2, s. 1158-1163
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Tidskriftsartikel (refereegranskat)abstract
- In this work a new method is presented to efficiently produce hydrogel scaffolds reinforced with nanofibers to show enhanced mechanical properties and improved structural integrity. The method is based on a combination of air brush spraying of a hydrogel and electrospinning of nanofibers. With air brush spraying the controllability is enhanced and the potential for scale-up increased. The developed method was used to successfully reinforce gellan gum hydrogels with electrospun polycaprolactone nanofibers. Optical and rheological evaluations were performed and showed that parameters such as the amount of incorporated nanofibers, gellan gum concentration and calcium chloride (crosslinker) concentrations could be used to modulate material properties. Incorporation of a small amount of nanofibers had a reinforcing effect and resulted in a hydrogel with rheological properties similar to the human nucleus pulposus (NP). The method is flexible and carries potential for designing scaffolds for e. g. NP tissue regeneration. (
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| 6. |
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| 7. |
- Thorvaldsson, Anna, 1981, et al.
(författare)
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Electrospinning of Highly Porous Scaffolds for Cartilage Regeneration
- 2008
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Ingår i: Biomacromolecules. - : American Chemical Society (ACS). - 1525-7797 .- 1526-4602. ; 9:3, s. 1044-1049
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Tidskriftsartikel (refereegranskat)abstract
- This study presents a new innovative method where electrospinning is used to coat single microfibers with nanofibers. The nanofiber-coated microfibers can be formed into scaffolds with the combined benefits of tailored porosity for cellular infiltration and nanostructured surface morphology for cell growth. The nanofiber coating is obtained by using a grounded collector rotating around the microfiber, to establish an electrical field yet allow collection of nanofibers on the microfiber. A Teflon tube surrounding the fibers and collector is used to force the nanofibers to the microfiber. Polycaprolactone nanofibers were electrospun onto polylactic acid microfibers and scaffolds of 95 and 97% porosities were made. Human chondrocytes were seeded on these scaffolds and on reference scaffolds of purely nanofibers and microfibers. Thereafter, cellular infiltration was investigated. The results indicated that scaffold porosity had great effects on cellular infiltration, with higher porosity resulting in increased infiltration, thereby confirming the advantage of the presented method. © 2008 American Chemical Society.
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| 8. |
- Thorvaldsson, Anna, 1981, et al.
(författare)
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Electrospinning of nanofibers for biomedical applications
- 2008
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Ingår i: 8th World Biomaterials Congress 2008, WBC 2008; Amsterdam; Netherlands; 28 May 2008 through 1 June 2008. - 9781615670802 ; 3, s. 1197-
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Konferensbidrag (refereegranskat)
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| 9. |
- Thorvaldsson, Anna, 1981, et al.
(författare)
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Superhydrophobic behaviour of plasma modified electrospun cellulose nanofiber-coated microfibers
- 2012
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Ingår i: Cellulose. - : Springer Science and Business Media LLC. - 0969-0239 .- 1572-882X. ; 19:5, s. 1743-1748
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Tidskriftsartikel (refereegranskat)abstract
- In this work, a method is presented for production of a textile cellulose fiber with non-wetting properties suitable for applications ranging from wound care and tissue engineering to clothing and other textile applications. Non-wettability is achieved by coating a textile cellulose microfiber with electrospun cellulose nanofibers, creating a large and rough surface area that is further plasma treated with fluorine plasma. High surface roughness and efficient deposition of covalently bound fluorine groups results in the fiber exhibiting non-wetting properties with contact angle measurements indicating superhydrophobicity (> 150A degrees water contact angle). It is an environmentally friendly method and the flexibility of the electrospinning process allows for careful design of material properties regarding everything from material choice and surface chemistry to fiber morphology and fiber assembly, pointing to the potential of the method and the developed fibers within a wide range of applications.
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| 10. |
- Thorvaldsson, Anna, 1981
(författare)
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Technology Development for Electrospinning of Novel Biomaterials Structures
- 2012
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- As the population gets older, the demands for new medical treatments increase since many of our most common diseases are related to age, including coronary heart disease, osteoarthritis and chronic ulcers. With this comes an increasing need for new biomaterial structures supporting and promoting tissue regeneration. The work in this thesis aims at developing new technologies based on electrospinning for creating nanofibrous biomaterial structures that mimic human tissues and are suitable for regeneration of damaged tissues and organs. As shown in this thesis, electrospinning has great potential as a design tool for creating body-mimicking nanofibrous materials with control over features from nano- to macro scale. The electrospinning process is highly flexible and allows for creation of nano- to micro sized fibers of a variety of materials and with a wide range of morphologies and structures. Also, there are possibilities of incorporating particles, tuning degradability and combining different materials of different properties. By combining electrospun nanofibers with larger structures (e.g. microfibers, metal surfaces, hydrogels), as described in this thesis, the possibilities are further increased as hierarchical structures are of great interest for the functionality of the material, e.g. the wetting and adhesion properties. This also allows for reinforcement of the otherwise mechanically weak nanofibers. It is shown in this work that electrospun nanofibers may act as reinforcement, despite the weak mechanical properties of the nanofibers, and are of interest for e.g. hydrogels to create possibly injectable scaffolds with appropriate mechanical stability. Porosity and pore sizes are other important limitations of electrospun nanofibrous materials used in biomedical applications. This is addressed in this thesis with the development of nanofiber-coated microfibers, textile fibers with a nanostructured surface which may find use in many different applications where their high surface area and textile processability can be beneficial. However, for tissue engineering purposes the possibility of using them to create highly porous scaffolds are of importance. As shown in the conducted cell studies, the high scaffold porosity allows for complete cell infiltration and study of cell response in relation to porosity and nanofibrous structures in ways that have so far not been possible.
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