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Sökning: WFRF:(Tichopad A)

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Numrering	Referens	Omslagsbild	Hitta
1.	Reitsma, MB, et al. (författare) Spatial, temporal, and demographic patterns in prevalence of smoking tobacco use and attributable disease burden in 204 countries and territories, 1990-2019: a systematic analysis from the Global Burden of Disease Study 2019 2021 Ingår i: Lancet (London, England). - : Elsevier. - 1474-547X .- 0140-6736. ; 397:10292, s. 2337-2360 Tidskriftsartikel (refereegranskat)
2.	Micah, AE, et al. (författare) Global investments in pandemic preparedness and COVID-19: development assistance and domestic spending on health between 1990 and 2026 2023 Ingår i: The Lancet. Global health. - : Elsevier. - 2214-109X. ; 11:3, s. E385-E413 Tidskriftsartikel (refereegranskat)
3.	Veiga, MI, et al. (författare) Antimalarial exposure delays Plasmodium falciparum intra-erythrocytic cycle and drives drug transporter genes expression 2010 Ingår i: PloS one. - : Public Library of Science (PLoS). - 1932-6203. ; 5:8, s. e12408- Tidskriftsartikel (refereegranskat)
4.	Tichopad, A, et al. (författare) Design and Optimization of Reverse-Transcription Quantitative PCR Experiments. 2009 Ingår i: Clinical chemistry. - : Oxford University Press (OUP). - 1530-8561 .- 0009-9147. ; 55:10, s. 1816-1823 Tidskriftsartikel (refereegranskat)abstract BACKGROUND: Quantitative PCR (qPCR) is a valuable technique for accurately and reliably profiling and quantifying gene expression. Typically, samples obtained from the organism of study have to be processed via several preparative steps before qPCR. METHOD: We estimated the errors of sample withdrawal and extraction, reverse transcription (RT), and qPCR that are introduced into measurements of mRNA concentrations. We performed hierarchically arranged experiments with 3 animals, 3 samples, 3 RT reactions, and 3 qPCRs and quantified the expression of several genes in solid tissue, blood, cell culture, and single cells. RESULTS: A nested ANOVA design was used to model the experiments, and relative and absolute errors were calculated with this model for each processing level in the hierarchical design. We found that intersubject differences became easily confounded by sample heterogeneity for single cells and solid tissue. In cell cultures and blood, the noise from the RT and qPCR steps contributed substantially to the overall error because the sampling noise was less pronounced. CONCLUSIONS: We recommend the use of sample replicates preferentially to any other replicates when working with solid tissue, cell cultures, and single cells, and we recommend the use of RT replicates when working with blood. We show how an optimal sampling plan can be calculated for a limited budget.

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Typ av publikation: tidskriftsartikel (4)

Typ av innehåll: refereegranskat (4)

Författare/redaktör: Ahmed, H. (2); Abbafati, C (2); Abbasi-Kangevari, M (2); Abedi, A (2); Aji, B (2); Alanzi, TM (2); visa fler...; Aljunid, SM (2); Alvis-Guzman, N (2); Ancuceanu, R (2); Arabloo, J (2); Azari, S (2); Baig, AA (2); Basu, S (2); Bhardwaj, P (2); Braithwaite, D (2); Dandona, L (2); Dandona, R (2); Dharmaratne, SD (2); El Tantawi, M (2); Faraon, EJA (2); Fischer, F (2); Foigt, NA (2); Ghafourifard, M (2); Ghashghaee, A (2); Golechha, M (2); Hay, SI (2); Heidari, G (2); Herteliu, C (2); Holla, R (2); Hosseinzadeh, M (2); Hostiuc, S (2); Ilesanmi, OS (2); Jakovljevic, M (2); Joo, T (2); Jurisson, M (2); Kalankesh, LR (2); Karimi, SE (2); Kosen, S (2); Koyanagi, A (2); Krishan, K (2); Kusuma, D (2); Majeed, A (2); Mansouri, B (2); Mansournia, MA (2); Meretoja, A (2); Meretoja, TJ (2); Mirrakhimov, EM (2); Mohammadian-Hafsheja ... (2); Mohammed, S (2); Mokdad, AH (2); visa färre...

Lärosäte: Karolinska Institutet (3); Uppsala universitet (2); Göteborgs universitet (1)

Språk: Engelska (4)

Forskningsämne (UKÄ/SCB): Medicin och hälsovetenskap (2)

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