| 1. |
- Thompson, Deborah J, et al.
(författare)
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Genetic predisposition to mosaic Y chromosome loss in blood
- 2019
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 575, s. 652-657
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Tidskriftsartikel (refereegranskat)abstract
- Mosaic loss of chromosome Y (LOY) in circulating white blood cells is the most common form of clonal mosaicism1-5, yet our knowledge of the causes and consequences of this is limited. Here, using a computational approach, we estimate that 20% of the male population represented in the UK Biobank study (n = 205,011) has detectable LOY. We identify 156 autosomal genetic determinants of LOY, which we replicate in 757,114 men of European and Japanese ancestry. These loci highlight genes that are involved in cell-cycle regulation and cancer susceptibility, as well as somatic drivers of tumour growth and targets of cancer therapy. We demonstrate that genetic susceptibility to LOY is associated with non-haematological effects on health in both men and women, which supports the hypothesis that clonal haematopoiesis is a biomarker of genomic instability in other tissues. Single-cell RNA sequencing identifies dysregulated expression of autosomal genes in leukocytes with LOY and provides insights into why clonal expansion of these cells may occur. Collectively, these data highlight the value of studying clonal mosaicism to uncover fundamental mechanisms that underlie cancer and other ageing-related diseases.
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| 2. |
- Darabi, H., et al.
(författare)
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Development of a novel hybrid multi-boosting neural network model for spatial prediction of urban flood
- 2021
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Ingår i: Geocarto International. - : Taylor and Francis Ltd.. - 1010-6049 .- 1752-0762.
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Tidskriftsartikel (refereegranskat)abstract
- In this study, a new hybridized machine learning algorithm for urban flood susceptibility mapping, named MultiB-MLPNN, was developed using a multi-boosting technique and MLPNN. The model was tested in Amol City, Iran, a data-scarce city in an ungauged area which is prone to severe flood inundation events and currently lacks flood prevention infrastructure. Performance of the hybridized model was compared with that of a standalone MLPNN model, random forest and boosted regression trees. Area under the curve, efficiency, true skill statistic, Matthews correlation coefficient, misclassification rate, sensitivity and specificity were used to evaluate model performance. In validation, the MultiB-MLPNN model showed the best predictive performance. The hybridized MultiB-MLPNN model is thus useful for generating realistic flood susceptibility maps for data-scarce urban areas. The maps can be used to develop risk-reduction measures to protect urban areas from devastating floods, particularly where available data are insufficient to support physically based hydrological or hydraulic models.
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| 4. |
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| 6. |
- Mobini Far, Hamid Reza, et al.
(författare)
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ELISA on a microchip with a photodiode for detection of amphetamine in plasma and urine
- 2005
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Ingår i: Journal of Analytical Toxicology. - : Oxford University Press (OUP). - 1945-2403 .- 0146-4760. ; 29:8, s. 790-793
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Tidskriftsartikel (refereegranskat)abstract
- A rapid and sensitive assay was developed for the detection of amphetamine in plasma and urine. The method relies on the principle of competitive ELISA (enzyme-linked immunosorbent assay). A flow microchip with a total volume of 7 µL was used for the development of a chemiluminescent ELISA technique. Solutions, samples, and the chemiluminescence substrate were injected by a flow system, and a photodiode detector was used to measure the light intensity. The incubation time of the competitor (competition phase) was reduced to 10 min. Calibration curves corresponding to analyte concentrations ranging from 40 to 1000 µg/L in urine samples and from 6 to 96 µg/L in plasma samples were obtained. The detection limits were in the region of 20 and 6 µg/L in urine and plasma, respectively. The main focus of the work was on speed, reliability, reproducibility, and operational stability of the assay. This method was proven readily adaptable to automation and provided reproducible results.
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| 7. |
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| 8. |
- Salminen, H, et al.
(författare)
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DXL HEEL BMD AND FUTURE FRACTURE RISK
- 2013
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Ingår i: OSTEOPOROSIS INTERNATIONAL. - 0937-941X. ; 24, s. S228-S229
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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| 9. |
- Torabi, F., et al.
(författare)
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Coulometric determination of NAD+ and NADH in normal and cancer cells using LDH, RVC and a polymer mediator
- 1999
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Ingår i: Talanta. - 1873-3573. ; 50:4, s. 787-797
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Tidskriftsartikel (refereegranskat)abstract
- An electrochemical method for the measurement of NAD(+) and NADH in normal and cancer tissues using flow injection analysis (FIA) is reported. Reticulated vitreous carbon (RVC) electrodes with entrapped L-lactate dehydrogenase (LDH) and a new redox polymer containing covalently bound toluidine blue O (TBO) were employed for this purpose. Both NAD(+) and NADH were estimated coulometrically based on their reaction with LDH. The latter was immobilized on controlled pore glass (CPG) by cross-linking with glutaraldehyde and packed within the RVC. The concentrations of NAD(+) and NADH in the tissues, estimated using different electron mediators such as ferricyanide (FCN), meldola blue (MB) and TBO have also been compared. The effects of flow rate, pH, applied potential (versus Ag/AgCl reference) and adsorption of the mediators have also been investigated. Based bn the measurements of NAD(+) and NADH in normal and cancer tissues it has been concluded that the NADH concentration is lower, while the NAD(+) concentration is higher in cancer tissues. Amongst the electron mediators TBO was found to be a more stable mediator for such measurements. (C) 1999 Elsevier Science B.V. All rights reserved.
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