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- Laine, Romain F., et al.
(författare)
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NanoJ : a high-performance open-source super-resolution microscopy toolbox
- 2019
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Ingår i: Journal of Physics D. - : IOP Publishing. - 0022-3727 .- 1361-6463. ; 52:16
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Forskningsöversikt (refereegranskat)abstract
- Super-resolution microscopy (SRM) has become essential for the study of nanoscale biological processes. This type of imaging often requires the use of specialised image analysis tools to process a large volume of recorded data and extract quantitative information. In recent years, our team has built an open-source image analysis framework for SRM designed to combine high performance and ease of use. We named it NanoJ-a reference to the popular ImageJ software it was developed for. In this paper, we highlight the current capabilities of NanoJ for several essential processing steps: spatio-temporal alignment of raw data (NanoJ-Core), super-resolution image reconstruction (NanoJ-SRRF), image quality assessment (NanoJ-SQUIRREL), structural modelling (NanoJ-VirusMapper) and control of the sample environment (NanoJ-Fluidics). We expect to expand NanoJ in the future through the development of new tools designed to improve quantitative data analysis and measure the reliability of fluorescent microscopy studies.
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| 2. |
- Mihailova, B., et al.
(författare)
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Raman scattering in locally inhomogeneous oxide crystals
- 2003
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Ingår i: Phase Transitions. - : Informa UK Limited. - 0141-1594 .- 1029-0338. ; 76:1-2, s. 17-32
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Tidskriftsartikel (refereegranskat)abstract
- Local-scale atomic arrangements in three types of oxide crystalline materials with structural defects, self-bonded molecular sieves, ferroelastics and relaxor ferroelectricity, are investigated by Raman spectroscopy. The experimentally observed spectral data are analysed through normal mode calculations for structural species representative of the intermediate range order in the solid. In the case of molecular sieves, the important role of incipient structural species for producing crystalline silicalite-1 and zeolite beta spheres of mm size is shown. In the case of ferroelastics, further insights on structural perturbation across the domain walls in monoclinic Pb 3(PO4)2 are provided. In the case of relaxors, the local structure and related dynamical effects in PbSc0.5Nb 0.5O3 and PbSc0.5Nb0.5O3 is revealed.
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