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  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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  • Coleman, Jim, et al. (författare)
  • Forum on English-medium Instruction
  • 2018
  • Ingår i: TESOL quarterly (Print). - : Wiley-Blackwell. - 0039-8322 .- 1545-7249. ; 52:3, s. 701-720
  • Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
    • During the first half of 2017, four scholars who know English-medium instruction (EMI) well and view it from different perspectives took part in a round-robin exchange. The forum contributors answered six sets of questions on aspects of EMI: defining terms; learning English in an EMI environment; the political or policy framework; learning a subject in a second language; teachers working in a second language; and EMI and multilingual education. Below is a summary of their answers (a longer version can be found in the online version of this Forum as supplementary content). The questions were devised and the responses edited by Philip Shaw.
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