| 1. |
|
|
| 2. |
|
|
| 3. |
- Beal, Jacob, et al.
(författare)
-
Robust estimation of bacterial cell count from optical density
- 2020
-
Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
-
Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
|
|
| 4. |
|
|
| 5. |
|
|
| 6. |
- Mantovani, G, et al.
(författare)
-
Recommendations for Diagnosis and Treatment of Pseudohypoparathyroidism and Related Disorders: An Updated Practical Tool for Physicians and Patients
- 2020
-
Ingår i: Hormone research in paediatrics. - : S. Karger AG. - 1663-2826 .- 1663-2818. ; 93:3, s. 182-196
-
Tidskriftsartikel (refereegranskat)abstract
- Patients affected by pseudohypoparathyroidism (PHP) or related disorders are characterized by physical findings that may include brachydactyly, a short stature, a stocky build, early-onset obesity, ectopic ossifications, and neurodevelopmental deficits, as well as hormonal resistance most prominently to parathyroid hormone (PTH). In addition to these alterations, patients may develop other hormonal resistances, leading to overt or subclinical hypothyroidism, hypogonadism and growth hormone (GH) deficiency, impaired growth without measurable evidence for hormonal abnormalities, type 2 diabetes, and skeletal issues with potentially severe limitation of mobility. PHP and related disorders are primarily clinical diagnoses. Given the variability of the clinical, radiological, and biochemical presentation, establishment of the molecular diagnosis is of critical importance for patients. It facilitates management, including prevention of complications, screening and treatment of endocrine deficits, supportive measures, and appropriate genetic counselling. Based on the first international consensus statement for these disorders, this article provides an updated and ready-to-use tool to help physicians and patients outlining relevant interventions and their timing. A life-long coordinated and multidisciplinary approach is recommended, starting as far as possible in early infancy and continuing throughout adulthood with an appropriate and timely transition from pediatric to adult care.
|
|
| 7. |
|
|
| 8. |
- Demirören, M., et al.
(författare)
-
A conversation analytic investigation into displays of expertise of different tutors in small group clinical skills training
- 2024
-
Ingår i: Linguistics and Education. - : Elsevier Ltd. - 0898-5898 .- 1873-1864. ; 79
-
Tidskriftsartikel (refereegranskat)abstract
- Clinical skills training requires a comprehensive understanding of how tutors demonstrate their knowledge, and fulfil their tutoring practices in medical education. This study aims to investigate expertise demonstration of different tutors following students' requests for information during clinical skills training. For this purpose, video recordings of the suturing skill training provided by three different tutors (medical expert, research assistant, and near-peer tutor) were analysed through Conversation Analysis. The findings show that medical expert showcases expertise knowledge through different practices such as using directives and providing assessments while the research assistant takes a more collaborative approach, involving peers in the learning process. The study also indicates that the near-peer tutor employs concise and less informative utterances, refrains from giving direct feedback. This research offers valuable insights into how tutors display expertise by documenting various interactional practices in clinical skills training, and providing data-driven implications for professional training programs in medical education.
|
|
| 9. |
- Dermenci, K.B., et al.
(författare)
-
Effect of cathode slurry composition on the electrochemical properties of Li-ion batteries
- 2015
-
Ingår i: ECS Transactions. - 1938-5862 .- 1938-6737. ; 66:9, s. 285-293
-
Tidskriftsartikel (refereegranskat)abstract
- The performance difference between commercial and laboratory scale cells remains a problem to be solved. Different way of battery electrode preparation is considered to be the main reason underlying various battery performance. In this work, the effect of slurry composition on electrochemical properties of Li-ion batteries is reported. Slurry preparation with various compositions of LiFePO4 active material (76-88%), PVdF binder (6-12%) and Super P Carbon conductive additive (6-12%) has been studied. Charge-discharge curves and capacity fade of electrodes are also investigated. Selected electrodes were pressed in order to see the effect of pressing on the final performance. Results showed that varying PVdF and carbon content mainly effects charge-discharge characteristics. For unpressed samples, higher amount of PVdF and carbon could result higher maximum specific capacity and lower internal resistance during lithiation and delithiation of positive electrode. Pressing reduces the distance between slurry particles, which enhances the conductivity of the prepared cell.
|
|
| 10. |
|
|
