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- Bergstrand, Eric, et al.
(författare)
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Detection of frequent neutrophil misclassification by the ProCyte Dx in sick dogs and how to avoid it
- 2022
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Ingår i: Journal of Small Animal Practice. - : Wiley. - 0022-4510 .- 1748-5827. ; 63, s. 603-608
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Tidskriftsartikel (refereegranskat)abstract
- Objectives A common and severe error in identifying neutrophils in feline blood samples by the IDEXX ProCyte Dx haematology analyser (ProCyte) has been reported. The hypothesis was that the same or similar error would be identified during analysis of canine blood samples and that white blood cell dot plot evaluation would be critical to detect and avoid erroneous results. Materials and Methods Eighty-six canine blood samples collected for clinical diagnosis of hospital patients were evaluated. Differential leukocyte counts were determined by the ProCyte Dx, ADVIA 2120 and manual methods. ProCyte neutrophil percentage results were considered unacceptable if the result was 15% different than percentage results from both ADVIA 2120 and manual counts. ProCyte WBC dot plots and instrument flags were evaluated for correctness. Results The ProCyte neutrophil counts were unacceptably lower than the ADVIA 2120 and manual neutrophil counts in 13 samples (15% of 86 samples). Neutrophils misclassified by the instrument were erroneously classified as monocytes and/or lymphocytes. All these samples were from patients with systemic inflammation. The error could be eliminated by rejecting results from samples with incorrect separation of cell clusters in the ProCyte WBC dot plots. Clinical Significance The ProCyte neutrophil count error with canine blood samples is common, severe and might affect clinical decisions. Operators of the instrument must evaluate white blood cell dot plots for correctness to avoid the error.
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| 2. |
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| 3. |
- Ekstrand, Carl, et al.
(författare)
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Plasma Dexamethasone Concentration in Relation to Glucose Response in the Horse
- 2019
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Ingår i: Journal of Equine Veterinary Science. - : Elsevier BV. - 0737-0806 .- 1542-7412. ; 73, s. 75-80
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Tidskriftsartikel (refereegranskat)abstract
- Therapeutic agents capable of altering the performance of horses are monitored in racing and equestrian sports to guarantee horse welfare, fair competition, and integrity of the sport. Dexamethasone is a common glucocorticoid drug for treating horses. Among other effects, dexamethasone is gluconeogenic and increases blood glucose. In this study, plasma samples from two dexamethasone exposure studies were analyzed for glucose using an automated clinical chemistry analyzer. In study one, dexamethasone-21-isonicotinate was administered to six horses at the dose 30 mu g/kg intramuscularly. In study two, dexamethasone 21-phosphate disodium salt was administered intravenously to six horses as a bolus dose followed by 3 hours of infusion (bolus + infusion) at four different doses (placebo, 0.1 + 0.07 mu g/kg, 1 + 0.7 mu g/kg, and 10 + 7 mu g/kg). Plasma dexamethasone concentrations were linked to plasma glucose concentrations by means of a turnover model. The median (range) pharmacodynamic parameters for glucose response for the two studies were as follows: the EC50 value was 0.84 mu g/L (0.47-1.50) and 0.85 mu g/L (0.75-2.45), the fractional turnover rate of response was 0.18 per h (0.07-0.27) and 0.25 per h (0.17-0.48), and the unaffected baseline of response was 4.20 mmol/L (4.10-6.60) and 5.42 mmol/L (5.22-5.96). These results may be used as input to future studies of the anti- inflammatory response of dexamethasone. The results also enable calculations of irrelevant plasma concentrations to determine whether the presence of a drug is a trace from legitimate medication or not. Therefore, this study provides further evidence for dexamethasone screening limits, which protects the integrity of the sport and the welfare of the horse. (C) 2018 Elsevier Inc. All rights reserved.
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| 4. |
- Falkenö, Ulrika, et al.
(författare)
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Anemia in a dog
- 2013
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Annan publikation (övrigt vetenskapligt/konstnärligt)
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| 5. |
- Falkenö, Ulrika, et al.
(författare)
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Theileria annae in a young Swedish dog
- 2013
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Ingår i: Acta Veterinaria Scandinavica. - : Springer Science and Business Media LLC. - 0044-605X .- 1751-0147. ; 55
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Tidskriftsartikel (refereegranskat)abstract
- A severe regenerative anemia was detected in a 12-week-old mixed breed puppy in Sweden. A small protozoan parasite was observed in erythrocytes on a blood smear. It was initially suspected to be Babesia gibsoni based on its size and because B. gibsoni was previously recorded in Sweden. Surprisingly, specific polymerase chain reaction analysis identified the protozoan as Theileria annae. T. annae is endemic in Northwest Spain, is very uncommonly reported elsewhere and has never been recorded in Scandinavia. T. annae has been identified in dogs used for dog fighting, and it is thought to be transmitted by dog bites. This puppy was a mixed pit bull terrier. Pit bull terriers are sometimes used for dog fighting. T. annae has been reported to be transmitted vertically, and in light of the puppy's age, this transmission was suspected in the present case.
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| 6. |
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| 7. |
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| 8. |
- Hillström, Anna, et al.
(författare)
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Evaluation of an in-clinic Serum Amyloid A (SAA) assay and assessment of the effects of storage on SAA samples
- 2010
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Ingår i: Acta Veterinaria Scandinavica. - : Springer Science and Business Media LLC. - 0044-605X .- 1751-0147. ; 52
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Tidskriftsartikel (refereegranskat)abstract
- Background: An in-clinic assay for equine serum amyloid A (SAA) analysis, Equinostic EVA1, was evaluated for use in a clinical setting. Stability of SAA in serum samples was determined.Methods: Intra-and inter-assay variation of the in-clinic method was determined. The in-clinic method (EVA1) results were compared to a reference method (Eiken LZ SAA) with 62 patient samples. For samples with SAA concentrations within the assay range of EVA1 (10-270 mg/L), differences between the methods were evaluated in a difference plot. Linearity under dilution was evaluated in two samples. Stability of SAA in three serum pools stored at 4 degrees C and approximately 22 degrees C was evaluated with the reference method day 0, 1, 2, 4, 7, 17 and analysed with a two-way ANOVA.Results: The imprecision (coefficient of variation, CV) for the in-clinic method was acceptable at higher SAA concentrations with CV values of 7,3-12%, but poor at low SAA concentrations with CV values of 27% and 37% for intra-and inter-assay variation respectively. Recovery after dilution was 50-138%. The in-clinic assay and the reference method identified equally well horses with low (< 10 mg/L) and high (> 270 mg/L) SAA concentrations. Within the assay range of the in-clinic method, 10-270 mg/L, the difference between the two methods was slightly higher than could be explained by the inherent imprecision of the assays. There were no significant changes of serum SAA concentrations during storage.Conclusions: The in-clinic assay identified horses with SAA concentrations of < 10 mg/L and > 270 mg/L in a similar way as the reference method, and provided an estimate of the SAA concentration in the range of 10-270 mg/L. The imprecision of the in-clinic method was acceptable at high SAA concentrations but not at low concentrations. Dilution of samples gave inconsistent results. SAA was stable both at room temperature and refrigerated, and thus samples may be stored before analysis with the reference method.
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| 9. |
- Hillström, Anna, et al.
(författare)
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Evaluation of cytologic findings in feline conjunctivitis
- 2012
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Ingår i: Veterinary Clinical Pathology. - 0275-6382 .- 1939-165X. ; 41, s. 283-290
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Tidskriftsartikel (refereegranskat)abstract
- Background Cytologic examination of smears prepared from ocular swabs of conjunctiva from cats with conjunctivitis permits identification of the type of inflammation and possibly specific microorganisms. Results of studies of the diagnostic utility of cytology for detection of infectious causes of feline conjunctivitis have been inconsistent. Objectives The objectives of this study were to describe cytologic findings in cats with conjunctivitis and to compare those findings with results of PCR analysis for feline herpesvirus (FHV-1), Chlamydophila felis (C felis), and Mycoplasma felis (M felis). Methods Conjunctival smears from 88 cats with conjunctivitis and 10 healthy control cats were stained with a Romanowsky stain and evaluated for the type of inflammation and evidence of an infectious agent. PCR analysis for FHV-1, C felis, and M felis was performed. Results Infectious agents identified by PCR analysis were FHV-1 in 9 cats (10%), C felis in 8 cats (9%), and M felis in 6 cats (7%). Inclusions interpreted as chlamydial inclusions were found in all cytologic smears from cats positive for C felis by PCR analysis and in 3 PCR-negative cats. Inclusions interpreted as Mycoplasma organisms were found in 3 of 6 cats that were PCR-positive for M felis and in 1 PCR-negative cat. FHV-1 inclusion bodies were not detected on cytologic examination. Conclusions Cytologic examination can be diagnostic for C felis infection when many typical inclusions are present. Cytologic examination was unreliable in diagnosing M felis infection, and viral inclusions of FHV-1 were not found in specimens stained with Romanowsky stains.
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| 10. |
- Hillström, Anna, et al.
(författare)
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Hereditary Phosphofructokinase Deficiency in Wachtelhunds
- 2011
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Ingår i: Journal Of The American Animal Hospital Association. - 0587-2871 .- 1547-3317. ; 47, s. 145-150
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Tidskriftsartikel (refereegranskat)abstract
- Hereditary phosphofructokinase (PFK) deficiency was diagnosed in two Wachtelhund dogs and suspected in three related Wachtelhund dogs with exercise intolerance, hemolytic anemia, and pigmenturia. Severe, persistent reticulocytosis in light of only mild anemia together with hemoglobinuria after strenuous exercise suggested PFK deficiency. Low erythrocyte PFK activity together with low 2,3-diphosphoglycerate concentrations and a high hemoglobin-oxygen affinity confirmed the diagnosis. The PFK deficiency is due to a single missense mutation in the muscle-type PFK M-PFK gene in English springer and American cocker spaniels, whippets, and mixed-breed dogs; however, these PFK-deficient Wachtelhunds do not have the same PFK mutation.
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