| 1. |
- Kumar, Rajender, et al.
(författare)
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Sequence, structure and functionality of pectin methylesterases and their use in sustainable carbohydrate bioproducts : A review
- 2023
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Ingår i: International Journal of Biological Macromolecules. - : Elsevier BV. - 0141-8130 .- 1879-0003. ; 244
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Tidskriftsartikel (refereegranskat)abstract
- Pectin methylesterases (PMEs) are enzymes that play a critical role in modifying pectins, a class of complex polysaccharides in plant cell walls. These enzymes catalyze the removal of methyl ester groups from pectins, resulting in a change in the degree of esterification and consequently, the physicochemical properties of the polymers. PMEs are found in various plant tissues and organs, and their activity is tightly regulated in response to developmental and environmental factors. In addition to the biochemical modification of pectins, PMEs have been implicated in various biological processes, including fruit ripening, defense against pathogens, and cell wall remodelling. This review presents updated information on PMEs, including their sources, sequences and structural diversity, biochemical properties and function in plant development. The article also explores the mechanism of PME action and the factors influencing enzyme activity. In addition, the review highlights the potential applications of PMEs in various industrial sectors related to biomass exploitation, food, and textile industries, with a focus on development of bioproducts based on eco-friendly and efficient industrial processes.
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| 2. |
- Ullah, Sadia Fida, et al.
(författare)
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An Experimental Framework for Developing Point-of-Need Biosensors : Connecting Bio-Layer Interferometry and Electrochemical Impedance Spectroscopy
- 2022
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Ingår i: Biosensors. - : MDPI AG. - 2079-6374. ; 12:11
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Tidskriftsartikel (refereegranskat)abstract
- Biolayer interferometry (BLI) is a well-established laboratory technique for studying biomolecular interactions important for applications such as drug development. Currently, there are interesting opportunities for expanding the use of BLI in other fields, including the development of rapid diagnostic tools. To date, there are no detailed frameworks for implementing BLI in target-recognition studies that are pivotal for developing point-of-need biosensors. Here, we attempt to bridge these domains by providing a framework that connects output(s) of molecular interaction studies with key performance indicators used in the development of point-of-need biosensors. First, we briefly review the governing theory for protein-ligand interactions, and we then summarize the approach for real-time kinetic quantification using various techniques. The 2020 PRISMA guideline was used for all governing theory reviews and meta-analyses. Using the information from the meta-analysis, we introduce an experimental framework for connecting outcomes from BLI experiments (K-D, k(on), k(off)) with electrochemical (capacitive) biosensor design. As a first step in the development of a larger framework, we specifically focus on mapping BLI outcomes to five biosensor key performance indicators (sensitivity, selectivity, response time, hysteresis, operating range). The applicability of our framework was demonstrated in a study of case based on published literature related to SARS-CoV-2 spike protein to show the development of a capacitive biosensor based on truncated angiotensin-converting enzyme 2 (ACE2) as the receptor. The case study focuses on non-specific binding and selectivity as research goals. The proposed framework proved to be an important first step toward modeling/simulation efforts that map molecular interactions to sensor design.
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| 3. |
- Ullah, Sadia Fida, et al.
(författare)
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Cell wall degradation: Microbial enzymes in service of sustainable agriculture
- 2024
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Ingår i: Microbial Technology for Agro-Ecosystems: Crop Productivity, Sustainability, and Biofortification. - : Elsevier. ; , s. 177-189
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- The growing population and environmental issues have raised concerns about food safety and sustainable production, where the use of negatively impacting traditional pesticides and agrochemicals is constantly debated. To limit environmental damage and to ensure the quantity and quality of food production, a new era of food industry is transforming to sustainable agriculture practices assisted by the use of microorganisms and biomolecular tools. Cell walls are the key structure for plant development and biomass accumulation in the form of polysaccharides and regulate the plant's relationship with the ecology. Cell wall integrity, remodeling and degradation are critical aspects of the plant-pathogen and plant-beneficial interactions and also take part in the recycling of organic matter. In this context, the use of microbial cell wall degrading enzymes (CWDEs) is a green alternative to traditional agrochemicals. This chapter discusses the different activities of CWDEs, their production and their potential application in plant productivity, protection and waste valorization.
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