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- Govindaraju, Indira, et al.
(författare)
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Microscopic and spectroscopic characterization of rice and corn starch
- 2020
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Ingår i: Microscopy research and technique (Print). - : John Wiley & Sons. - 1059-910X .- 1097-0029. ; 83:5, s. 490-498
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Tidskriftsartikel (refereegranskat)abstract
- Starch granules from rice and corn were isolated, and their molecular mechanism on interaction with alpha-amylase was characterized through biochemical test, microscopic imaging, and spectroscopic measurements. The micro-scale structure of starch granules were observed under an optical microscope and their average size was in the range 1-100 mu m. The surface topological structures of starch with micro-holes due to the effect of alpha- amylase were also visualized under scanning electron microscope. The crystallinity was confirmed by X-ray diffraction patterns as well as second-harmonic generation microscopy. The change in chemical bonds before and after hydrolysis of the starch granules by alpha- amylase was determined by Fourier transform infrared spectroscopy. Combination of microscopy and spectroscopy techniques relates structural and chemical features that explain starch enzymatic hydrolysis which will provide a valid basis for future studies in food science and insights into the energy transformation dynamics.
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