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- Cerovic, V, et al.
(författare)
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Lymph-borne CD8α(+) dendritic cells are uniquely able to cross-prime CD8(+) T cells with antigen acquired from intestinal epithelial cells.
- 2015
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Ingår i: Mucosal immunology. - : Elsevier BV. - 1935-3456 .- 1933-0219. ; 8, s. 38-48
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Tidskriftsartikel (refereegranskat)abstract
- Cross-presentation of cellular antigens is crucial for priming CD8(+) T cells, and generating immunity to intracellular pathogens-particularly viruses. It is unclear which intestinal phagocytes perform this function in vivo. To address this, we examined dendritic cells (DCs) from the intestinal lymph of IFABP-tOVA 232-4 mice, which express ovalbumin in small intestinal epithelial cells (IECs). Among lymph DCs (LDCs) only CD103(+) CD11b(-) CD8α(+) DCs cross-present IEC-derived ovalbumin to CD8(+) OT-I T cells. Similarly, in the mesenteric lymph nodes (MLNs), cross-presentation of IEC-ovalbumin was limited to the CD11c(+) MHCII(hi) CD8α(+) migratory DCs, but absent from all other subsets, including the resident CD8α(hi) DCs. Crucially, delivery of purified CD8α(+) LDCs, but not other LDC subsets, into the MLN subcapsular lymphatic sinus induced proliferation of ovalbumin-specific, gut-tropic CD8(+) T cells in vivo. Finally, in 232-4 mice treated with R848, CD8α(+) LDCs were uniquely able to cross-prime interferon γ-producing CD8(+) T cells and drive their migration to the intestine. Our results clearly demonstrate that migrating CD8α(+) intestinal DCs are indispensable for cross-presentation of cellular antigens and, in conditions of inflammation, for the initial differentiation of effector CD8(+) T cells. They may therefore represent an important target for the development of antiviral vaccinations.Mucosal Immunology advance online publication, 21 May 2014; doi:10.1038/mi.2014.40.
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| 4. |
- Xie, Gongnan, et al.
(författare)
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Augmented Heat Transfer Of An Internal Blade Tip Wall With Pin-Fins
- 2009
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Ingår i: Proceedings Of The Asme Turbo Expo 2009, Vol 3, Pts A And B. - 9780791848845 ; , s. 361-369
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Konferensbidrag (refereegranskat)abstract
- The heat transferred to the turbine blade is substantially increased as the turbine inlet temperature is increased. Cooling methods are therefore much needed for the turbine blades to ensure a long durability and safe operation. The blade tip region is exposed to the hot gas flows. A common way to cool the tip is to use serpentine passages with 180-deg turn under the blade tip cap taking advantage of the three-dimensional turning effect and impingement. Improving internal convective cooling is therefore required to increase the blade tip life. In this paper, augmented heat transfer of a blade tip has been investigated numerically. The computational models consist of a two-pass channel with 180-deg turn and an array of pin-fins mounted on the tip-cap, and a smooth two-pass channel. Inlet Reynolds numbers are ranging from 100,000 to 600,000. The computations are 3D, steady, incompressible and stationary. The detailed 3D fluid flow and heat transfer over the tip surfaces are presented. The overall performance of the two models is evaluated. It is found that the pin-fins make the counter-rotating vortices towards pin-fin surfaces, resulting in continuous turbulent mixing near the pin-finned tip. Due to the combination of turning, impingement and pin-fin crossflow, the heat transfer coefficient of the pin-finned tip is a factor of as much as 1.84 higher than that of a smooth tip. This augmentation is achieved at the expense of a penalty of pressure drop around 35%. It is suggested that the pin-fins could be used to enhance blade tip heat transfer and cooling.
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| 5. |
- Xie, Gongnan, et al.
(författare)
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Effect of Pin Base-Fillet on Heat Transfer Enhancement of an Internal Blade Pin-Finned Tip-Wall
- 2009
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Ingår i: HT2009: Proceedings of the Asme Summer Heat Transfer Conference 2009, Vol 2. - 9780791843574 ; , s. 517-527
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Konferensbidrag (refereegranskat)abstract
- A common way to cool the tip is to use serpentine passages with 180-deg turn under the blade tip-cap. Improving internal convective cooling is therefore required to increase the blade tip life. In this paper, augmented heat transfer of a blade tip has been investigated numerically. The computational models consist of a two-pass channel with 180-deg turn and pin-fins mounted on the tip-cap, and a smooth two-pass channel. On the other hand, In particular manufacture, the casting process does not make a perfect cylinder pin, a fillet needs to be placed at the endwall. In order to make the conditions of simulations as close to real practice as possible, it is desirable to examine the effect of fillet on the tip heat transfer. Therefore, in the present study, the effect of pin base-fillet on heat transfer enhancement of a blade pin-finned tip-wall is investigated numerically. Inlet Reynolds numbers are ranging from 100,000 to 600,000. The computations are 3D, steady, incompressible and stationary. It is found that the pin-fins make the counter-rotating vortices towards pin-fin surfaces, resulting in continuous turbulent mixing near the pin-finned tip. Due to the combination of turning, impingement and pin-fin crossflow, the heat transfer coefficient of the pin-finned tip is a factor of as much as 2.66 higher than that of a smooth tip. Besides, with base-fillets the heat transfer enhancement is increased by about 10% while almost no additional pressure loss is resulted. It is suggested that the pin-fins could be used to enhance blade tip heat transfer and cooling.
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