| 1. |
- Francia, Marcos Gabriel, et al.
(författare)
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AKT1 induces Nanog promoter in a SUMOylation-dependent manner in different pluripotent contexts
- 2023
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Ingår i: BMC Research Notes. - 1756-0500. ; 16:1
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Tidskriftsartikel (refereegranskat)abstract
- AKT/PKB is a kinase crucial for pluripotency maintenance in pluripotent stem cells. Multiple post-translational modifications modulate its activity. We have previously demonstrated that AKT1 induces the expression of the pluripotency transcription factor Nanog in a SUMOylation-dependent manner in mouse embryonic stem cells. Here, we studied different cellular contexts and main candidates that could mediate this induction. Our results strongly suggest the pluripotency transcription factors OCT4 and SOX2 are not essential mediators. Additionally, we concluded that this induction takes place in different pluripotent contexts but not in terminally differentiated cells. Finally, the cross-matching analysis of ESCs, iPSCs and MEFs transcriptomes and AKT1 phosphorylation targets provided new clues about possible factors that could be involved in the SUMOylation-dependent Nanog induction by AKT.
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| 2. |
- Drouin-Ouellet, Janelle, et al.
(författare)
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The 2022 International Society for Stem Cell Research (ISSCR) Annual Meeting : Celebrating 20 Years of Achievements
- 2022
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Ingår i: Cellular Reprogramming. - : Mary Ann Liebert Inc. - 2152-4971 .- 2152-4998. ; 24:5, s. 212-222
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Tidskriftsartikel (refereegranskat)abstract
- Last June, the stem cell community came together to celebrate the 20th anniversary of the International Society for Stem Cell Research (ISSCR), one of the leading organizations in the field. The hybrid event mixed a varied program filled with plenary talks, concurrent track sessions, poster presentations, exhibit booths, and plenty of opportunities to enhance stem cell research through bonding between academia and industry. This report highlights the Plenary sessions, with the main topics discussed by each speaker. All the impressive research showcased during the meeting is genuine proof of the great advancements the field has witnessed during these last 20 years, and the more to come.
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| 3. |
- Echegaray, Camila Vazquez
(författare)
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Unleashing Ascl1 : Exploring Cross-Lineage Potential in Reprogramming and Regenerative Frontiers
- 2023
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Ingår i: Cellular Reprogramming. - 2152-4971. ; 25:4, s. 139-141
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Tidskriftsartikel (refereegranskat)abstract
- In the era of stem cell research and regenerative medicine, understanding the regulatory networks that drive cellular reprogramming is fundamental. The study entitled ‘‘Cross-lineage potential of Ascl1 uncovered by comparing diverse reprogramming regulatomes’’ published in Stem Cell Reports sheds light on the remarkable versatility of Ascl1, a transcription factor known for its pivotal role in neurogenesis. By comparing regulatomes across multiple cell lineages, the authors have elucidated the potential of Ascl1 to facilitate the conversion of non-neural cells into various lineages beyond its canonical neural fate, suggesting its potential as a master regulator for lineage reprogramming. These observations challenge our current understanding of cell fate determination and open exciting avenues for regenerative medicine.
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| 4. |
- Lin, Hsiu Chuan, et al.
(författare)
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Programming human cell fate : overcoming challenges and unlocking potential through technological breakthroughs
- 2023
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Ingår i: Development (Cambridge). - 0950-1991. ; 150:24
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Tidskriftsartikel (refereegranskat)abstract
- In recent years, there have been notable advancements in the ability to programme human cell identity, enabling us to design and manipulate cell function in a Petri dish. However, current protocols for generating target cell types often lack efficiency and precision, resulting in engineered cells that do not fully replicate the desired identity or functional output. This applies to different methods of cell programming, which face similar challenges that hinder progress and delay the achievement of a more favourable outcome. However, recent technological and analytical breakthroughs have provided us with unprecedented opportunities to advance the way we programme cell fate. The Company of Biologists' 2023 workshop on 'Novel Technologies for Programming Human Cell Fate' brought together experts in human cell fate engineering and experts in single-cell genomics, manipulation and characterisation of cells on a single (sub)cellular level. Here, we summarise the main points that emerged during the workshop's themed discussions. Furthermore, we provide specific examples highlighting the current state of the field as well as its trajectory, offering insights into the potential outcomes resulting from the application of these breakthrough technologies in precisely engineering the identity and function of clinically valuable human cells.
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| 5. |
- Silvério-Alves, Rita, et al.
(författare)
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GATA2 mitotic bookmarking is required for definitive haematopoiesis
- 2023
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Ingår i: Nature Communications. - 2041-1723. ; 14:1
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Tidskriftsartikel (refereegranskat)abstract
- In mitosis, most transcription factors detach from chromatin, but some are retained and bookmark genomic sites. Mitotic bookmarking has been implicated in lineage inheritance, pluripotency and reprogramming. However, the biological significance of this mechanism in vivo remains unclear. Here, we address mitotic retention of the hemogenic factors GATA2, GFI1B and FOS during haematopoietic specification. We show that GATA2 remains bound to chromatin throughout mitosis, in contrast to GFI1B and FOS, via C-terminal zinc finger-mediated DNA binding. GATA2 bookmarks a subset of its interphase targets that are co-enriched for RUNX1 and other regulators of definitive haematopoiesis. Remarkably, homozygous mice harbouring the cyclin B1 mitosis degradation domain upstream Gata2 partially phenocopy knockout mice. Degradation of GATA2 at mitotic exit abolishes definitive haematopoiesis at aorta-gonad-mesonephros, placenta and foetal liver, but does not impair yolk sac haematopoiesis. Our findings implicate GATA2-mediated mitotic bookmarking as critical for definitive haematopoiesis and highlight a dependency on bookmarkers for lineage commitment.
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