| 1. |
|
|
| 2. |
- Ndubuisil, MacKevin I, et al.
(författare)
-
Characterization of a Novel Mammalian Phosphatase Having Sequence Similarity to Schizosaccharomyces pombe PHO2 and Saccharomyces cerevisiae PHO13
- 2002
-
Ingår i: Biochemistry. - : American Chemical Society (ACS). - 0006-2960 .- 1520-4995. ; 41:24, s. 7841-8
-
Tidskriftsartikel (refereegranskat)abstract
- p34, a specific p-nitrophenyl phosphatase (pNPPase) was identified and purified from the murine cell line EL4 in a screen for the intracellular molecular targets of the antiinflammatory natural product parthenolide. A BLAST search analysis revealed that it has a high degree of sequence similarity to two yeast alkaline phosphatases. We have cloned, sequenced, and expressed p34 as a GST-tagged fusion protein in Escherichia coli and an EE-epitope-tagged fusion protein in mammalian cells. Using p-nitrophenyl phosphate (pNPP) as a substrate, p34 is optimally active at pH 7.6 with a Km of 1.36 mM and Kcat of 0.052 min-1. Addition of 1 mM Mg2+ to the reaction mixture increases its activity by 14-fold. Other divalent metal ions such as Co2+ and Mn2+ also stimulated the activity of the enzyme, while Zn2+, Fe2+, and Cu2+ had no effect. Furthermore, both NaCl and KCl enhanced the activity of the enzyme, having maximal effect at 50 and 75 mM, respectively. The enzyme is inhibited by sodium orthovanadate but not by sodium fluoride or okadaic acid. Mutational analysis data suggest that p34 belongs to the group of phosphatases characterized by the sequence motif DXDX(T/V).
|
|
| 3. |
- Roura-Pascual, Núria, et al.
(författare)
-
Alternative futures for global biological invasions
- 2021
-
Ingår i: Sustainability Science. - : Springer Science and Business Media LLC. - 1862-4065 .- 1862-4057. ; 16:5, s. 1637-1650
-
Tidskriftsartikel (refereegranskat)abstract
- Scenario analysis has emerged as a key tool to analyze complex and uncertain future socio-ecological developments. However, currently existing global scenarios (narratives of how the world may develop) have neglected biological invasions, a major threat to biodiversity and the economy. Here, we use a novel participatory process to develop a diverse set of global biological invasion scenarios spanning a wide range of plausible global futures through to 2050. We adapted the widely used two axes scenario analysis approach to develop four families of four scenarios each, resulting in 16 scenarios that were later clustered into four contrasting sets of futures. Our analysis highlights that socioeconomic developments and technological innovation have the potential to shape biological invasions, in addition to well-known drivers, such as climate and human land use change and global trade. Our scenarios partially align with the shared socioeconomic pathways created by the climate change research community. Several factors that drive differences in biological invasions were underrepresented in the shared socioeconomic pathways; in particular, the implementation of biosecurity policies. We argue that including factors related to public environmental awareness and technological and trade development in global scenarios and models is essential to adequately consider biological invasions in global environmental assessments and thereby obtain a more integrative picture of future social-ecological developments.
|
|
| 4. |
- Söderlund, Ulf, et al.
(författare)
-
U-Pb baddeleyite ages and Hf, Nd isotope chemistry constraining repeated mafic magmatism in the Fennoscandian Shield from 1.6 to 0.9 Ga
- 2005
-
Ingår i: Contributions to Mineralogy and Petrology. - : Springer Science and Business Media LLC. - 0010-7999 .- 1432-0967. ; 150:2, s. 174-194
-
Tidskriftsartikel (refereegranskat)abstract
- The Palaeoproterozoic (1.90 - 1.60 Ga) crust of central Fennoscandia was intruded repeatedly by dolerite dikes and sills during the Neo- and Mesoproterozoic eons. We report 17 new baddeleyite U - Pb dates comprising six generations of dolerites ( in Ma): Blekinge-Dalarna dolerites 946 - 978 Protogine Zone dolerites 1,215 - 1,221 Central Scandinavian Dolerite Group 1,264 - 1,271 Tuna dikes and age equivalents in Dalarna 1,461 - 1,462 Varmland dolerites similar to 1,568 Breven-Hallefors dolerites similar to 1,595 The favoured tectonic model implies that the majority of these suites were related to active margin processes somewhere west ( and possibly south) of the Fennoscandian Shield. Dolerite intrusions are interpreted to reflect discrete events of back-arc extension as the arc retreated oceanward. Initial Hf and Nd isotope compositions of the dolerite swarms fall between CHUR and normal-depleted mantle, and suggest a variably depleted and re-enriched mantle as the source for the here investigated 1.6 to 0.95 Ga old mafic rocks. Repeated recycling of older crustal components, mainly sediments ( dominated by material with short residence ages) in earlier subduction systems may have been very efficient at producing geochemically and isotopically variably enriched lithospheric mantle sections beneath the Fennoscandian Shield.
|
|
