| 1. |
- Gellein, Kristin, et al.
(author)
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Separation of proteins including metallothionein in cerebrospinal fluid by size exclusion HPLC and determination of trace elements by HR-ICP-MS
- 2007
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In: Brain Research. - : Elsevier BV. - 0006-8993 .- 1872-6240. ; 1174, s. 136-142
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Journal article (peer-reviewed)abstract
- A method to study the protein binding patterns of trace elements in human cerebrospinal fluid (CSF) is described. Proteins in CSF samples were separated by size exclusion chromatography combined with high performance liquid chromatography (SEC-HPLC). The column was calibrated to separate proteins in the molecular weight range 6-70 kDa. Fractions were then analyzed off-line for trace elements using high resolution inductively coupled plasma mass spectrometry (HR-ICP-MS). We were able to accurately determine more than 10 elements of clinical interest in the CSF fractions. Results are presented for Cd, Mn, Fe, Pb, Cu and Zn. The total concentrations of 16 trace elements in human plasma and CSF are also presented. The method was able to differentiate the relative contribution of metallothionein and other proteins towards metal binding in human CSF.
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| 2. |
- Mattsson, Magnus, et al.
(author)
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Mixing and displacement ventilation compared in classrooms; distribution of particles, cat allergen and CO2
- 2003
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In: Proceedings from Healthy Buildings 2003. ; , s. 458-464
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Conference paper (peer-reviewed)abstract
- Mixing ventilation and displacement ventilation were compared in an intervention study in classrooms. Particles, cat allergen and CO2, were measured in classroom air at different levels above the floor, during regular lessons. With mixing ventilation, the particle concentration tended to decrease with height, with a stronger gradient occurring for larger particles. With displacement ventilation, the particle concentration increased with height, except for particles >25 µm. The displacement system thus tended to have a slight upward displacement effect on most of the particles. Significant correlations were found between concentrations of cat allergen and particles in the size fraction 1–10 µm. The particle and cat allergen concentration at breathing height did not, however, differ significantly between the two ventilation systems. CO2 was about 10% lower with displacement ventilation. A fairly high level of physical activity of the pupils is believed to have had significant dispersing effect on the airborne contaminants.
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| 3. |
- Mattsson, Magnus, 1964-, et al.
(author)
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Ventilation filter efficiency for birch pollen allergens : experimental data from one pollen season
- 2011
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In: Proceedings from Roomvent 2011. - Trondheim : Tapir Akademisk Forlag. - 9788251928120
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Conference paper (peer-reviewed)abstract
- In an experimental study, ventilation filters of high quality (F7 & F9) were tested regarding their efficiency in collecting birch pollen allergens in outdoor air. The birch pollen grain concentration in outdoor air was measured at the same time as pollen allergen and particle number concentrations were measured before and after the tested ventilation filters, thus enabling collection efficiency calculations. Simultaneously, the size distribution of birch pollen allergens was measured in outdoor air using a cascade impactor. The study confirms previous indications that pollen allergens may occur in outdoor air in particles much smaller than pollen grains, and can penetrate ventilation filters to a larger extent than might be expected. This entails that although the high quality filters collect most of outdoor air pollen allergens, a significant exposure dose to these allergens can occur in the indoor environment, especially when considering the fact most people stay much more indoors than outdoors. The study also confirms previous similar indications attained with grass pollen allergens, in that the allergenic particles tend to penetrate ventilation filters to a greater extent than other airborne particles.
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| 4. |
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| 5. |
- Mattsson, Magnus, 1964-, et al.
(author)
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Ventilation filter efficiency for particles and grass pollen allergens
- 2009
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In: Proceedings of the 9th International Healthy Buildings Conference and Exhibition.
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Conference paper (peer-reviewed)abstract
- In an experimental study, commonly used ventilation fine filters were tested regarding their efficiency in collecting airborne particles and grass pollen allergens from outdoor air. Grass pollen allergen and particle number concentrations were measured before and after the filters, enabling collection efficiency calculations. Simultaneously, the size distribution of grass pollen allergens was measured in outdoor air using a cascade impactor. The study confirms previous indications that pollen allergens occur in the outdoor air as particles much smaller than pollen grains, and can penetrate ventilation filters to a larger extent than might be expected. The initially high collection efficiency of synthetic, electrostatically charged filters declined significantly with time of use (dust load), whereas glass fiber filters showed steady performance. A slight tendency for pollen allergenic matter to penetrate ventilation filters more than other airborne particles was noted, but no difference in the response to electrostatic charge of filters could be seen.
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| 6. |
- Roos, Per M., et al.
(author)
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Manganese in cerebrospinal fluid and blood plasma of patients with amyotrophic lateral sclerosis
- 2012
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In: Experimental biology and medicine. - : SAGE Publications. - 1535-3702 .- 1535-3699. ; 237:7, s. 803-810
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Journal article (peer-reviewed)abstract
- Neurotoxic properties of manganese (Mn) are well documented. It is less known that Mn contributes to the development of neurodegenerative disorders in the general population. This study presents Mn data from patients with amyotrophic lateral sclerosis (ALS) in a well-defined cohort diagnosed by electrophysiological methods. Cerebrospinal fluid (CSF) and plasma were collected from patients and controls. Mn concentrations were analyzed by high-resolution inductively coupled plasma mass spectrometry. Concentrations of Mn were significantly higher in ALS CSF (median 5.67 mu g/L) than in CSF from controls (median 2.08 mu g/L). Also, ALS CSF Mn concentrations were higher than ALS plasma Mn concentrations (median 0.91 mu g/L), suggesting transport of Mn into the central nervous system. The properties of barrier systems between blood and the brain are discussed and the possibility of Mn accumulation contributing to the relentless course of ALS is introduced.
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| 7. |
- Roos, Per M., et al.
(author)
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Metal Concentrations in Cerebrospinal Fluid and Blood Plasma from Patients with Amyotrophic Lateral Sclerosis
- 2013
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In: Biological Trace Element Research. - : Springer Science and Business Media LLC. - 0163-4984 .- 1559-0720. ; 151:2, s. 159-170
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Journal article (peer-reviewed)abstract
- Amyotrophic lateral sclerosis (ALS) is a progressive and fatal degenerative disorder of motor neurons. The cause of this degeneration is unknown, and different causal hypotheses include genetic, viral, traumatic and environmental mechanisms. In this study, we have analyzed metal concentrations in cerebrospinal fluid (CSF) and blood plasma in a well-defined cohort (n = 17) of ALS patients diagnosed with quantitative electromyography. Metal analyses were performed with high-resolution inductively coupled plasma mass spectrometry. Statistically significant higher concentrations of manganese, aluminium, cadmium, cobalt, copper, zinc, lead, vanadium and uranium were found in ALS CSF compared to control CSF. We also report higher concentrations of these metals in ALS CSF than in ALS blood plasma, which indicate mechanisms of accumulation, e.g. inward directed transport. A pattern of multiple toxic metals is seen in ALS CSF. The results support the hypothesis that metals with neurotoxic effects are involved in the pathogenesis of ALS.
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