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- Carol, R. J., et al.
(författare)
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PASTICCINO1 (AtFKBP70) is a nuclear-localised immunophilin required during Arabidopsis thaliana embryogenesis
- 2001
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Ingår i: Plant Science. - : Elsevier BV. - 0168-9452 .- 1873-2259. ; 161:3, s. 527-535
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Tidskriftsartikel (refereegranskat)abstract
- The PASTICCINO1 (PAS1) gene of Arabidopsis thaliana encodes a protein with homology to the FK506-binding protein (FKBP) class of immunophilins. To begin to understand more about the possible function of PAS1, we tested some properties of recombinant PAS1 protein and analysed the expression of the gene in Arabidopsis embryos and cell cultures and in tobacco cells. In pas1-1/+ heterozygote embryos the pas1-1 allele is expressed at very low levels in all cells, but it is misexpressed in the pas1-1 homozygote mutant at the same stage. Anti-PAS1 affinity-purified antibodies recognise a 70 kDa protein from dividing cell cultures of Arabidopsis. In indirect immunofluorescence, the same antibodies label the nuclei of dividing tobacco BY-2 cells. In a protease-coupled assay, recombinant PAS1 protein has low peptidylprolyl cis-trans isomerase (PPIase) activity, which is inhibited by the immunosuppressive drugs FK506 and rapamycin, but not by cyclosporin. PAS1 also binds calmodulin in vitro. This data suggests the importance of the correctly regulated production of functional PAS1 protein, a likely nuclear-localised FKBP, for the correct development of the plant embryo. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
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| 2. |
- Cowling, R. J., et al.
(författare)
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The role of PASTICCINO1, an FKBP-like protein, in plant development
- 1999
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- The pasticcino (pas) mutants of Arabidopsis thaliana were identified due to their abnormal response to cytokinins. These mutants fall into 3 complementation groups: pas1 (2 alleles), pas2 (1 allele) and pas3 (4 alleles). The mutants all have excessive and disorganised cell divisions leading to extra cell layers in the hypocotyl, abnormal apical meristems, and rosettes with fused vitreous leaves. This cell proliferation effect is exaggerated in the presence of cytokinins. The PAS1 gene was cloned via a T-DNA insertion; it encodes for a protein with similarities to FK506-binding proteins (FKBPs), a class of immunophilins. Like other large FKBPs the PAS1 protein has several putative FKBP domains, a tetratricopeptide repeat domain and a calmodulin binding motif. PAS1 also has conserved nuclear localisation signals. Although the presence of immunophilins has been demonstrated in plants, their functions are unknown. PAS1 is the first plant immunophilin-like gene to be disrupted and suggests a role for PAS 1 in the control of plant cell division.
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| 3. |
- Faure, J. D., et al.
(författare)
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The PASTICCINO genes of Arabidopsis thaliana are involved in the control of cell division and differentiation
- 1998
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Ingår i: Development. - 0950-1991 .- 1477-9129. ; 125:5, s. 909-918
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Tidskriftsartikel (refereegranskat)abstract
- The control of cell division by growth regulators is critical to proper plant development. The isolation of single-gene mutants altered in the response to plant hormones should permit the identification of essential genes controlling the growth and development of plants. We have isolated mutants pasticcino belonging to 3 complementation groups (pas1, pas2, pas3) in the progeny of independent ethyl methane sulfonate and T-DNA mutagenized Arabidopsis thaliana plants. The screen was performed in the presence or absence of cytokinin. The mutants isolated were those that showed a significant hypertrophy of their apical parts when grown on cytokinin-containing medium. The pas mutants have altered embryo, leaf and root development. They display uncoordinated cell divisions which are enhanced by cytokinin. Physiological and biochemical analyses show that cytokinins are probably involved in pas phenotypes. The PAS genes have been mapped respectively to chromosomes 3, 5 and 1 and represent new plant genes involved in the control of cell division and plant development.
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