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Sökning: WFRF:(Waetzig Hermann)

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1.
  • El Deeb, Sami, et al. (författare)
  • Recent advances in capillary electrophoretic migration techniques for pharmaceutical analysis (2013-2015)
  • 2016
  • Ingår i: Electrophoresis. - : Wiley. - 0173-0835 .- 1522-2683. ; 37:12, s. 1591-1608
  • Forskningsöversikt (refereegranskat)abstract
    • This review updates and follows-up a previous review by highlighting recent advancements regarding capillary electromigration methodologies and applications in pharmaceutical analysis. General approaches such as quality by design as well as sample injection methods and detection sensitivity are discussed. The separation and analysis of drug-related substances, chiral CE, and chiral CE-MS in addition to the determination of physicochemical constants are addressed. The advantages of applying affinity capillary electrophoresis in studying receptor-ligand interactions are highlighted. Finally, current aspects related to the analysis of biopharmaceuticals are reviewed. The present review covers the literature between January 2013 and December 2015.
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2.
  • van der Burg, Debbie, et al. (författare)
  • Analysis of Cationic Vitamins in Cell Culture Medium Samples by Capillary Zone Electrophoresis
  • 2022
  • Ingår i: Journal of Analytical Methods in Chemistry. - : Hindawi Limited. - 2090-8865 .- 2090-8873. ; 2022, s. 1-7
  • Tidskriftsartikel (refereegranskat)abstract
    • This paper describes a capillary electrophoresis method for the determination of the cationic B-vitamins thiamine, nicotinamide, pyridoxine, pyridoxal, and pyridoxamine in untreated cell culture medium samples. The effects of the buffering capacity, the mobility of the coion, and the preconditioning solution on the robustness of the method were investigated. Using a 100 mM phosphoric acid and 55 mM triethanolamine background electrolyte at pH 2.3 and capillary preconditioning with 1 M NaOH, all five vitamins could be separated with good resolution. Preliminary method validation data over the range 10-110 mu M for undiluted samples, with 10 mu M being the lower range limit of quantification QL, showed accuracy recoveries of 94-104%, and migration time and peak area repeatabilities within 0.4% RSD and 2.6% RSD, respectively.
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