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- Grimvall, Anders, et al.
(författare)
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Phytotoxic Substances in Runoff from Forested Catchment Areas
- 1991
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Ingår i: Humic substances in the aquatic and terrestrial environment : proceedings of an international symposium, Linköping, Sweden, August 21-23, 1989. - Berlin Heidelberg New York : Springer. - 3540537023 ; , s. 397-406
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Bokkapitel (refereegranskat)abstract
- Runoff from different catchment areas in southem Sweden was tested in a root bioassay based on solution cultures of cucumber seedlings. Water samples from agricultural catchment areas produced no signs at all or only weak signs of inhibited root growth, whereas several water samples from catchmcnt areas dominated by mires or coniferous forests produced visible root injuries. The most severe root injuries (very short roots, discolouration, swelling of root tips and lack of root hairs) were caused by samples from a catchment area without local emissions and dominated by old stands of spruce. Fractionation by ultrafiltration showed that the phytotoxic effect of these samples could be attributed to organic matter with a nominal molecular-weight exceeding 1000 or to substances associated with organic macromolecules. Experiments aimed at concentrating phytotoxic compounds from surface water indicated that the observed growth inhibition was caused by strongly hydrophilic substances. Previous reports on phytotoxic, organic substances of natura! origin have emphasized interaction between plants growing close together. The presence of phytotoxic substances in runoff indicates that there is also a large-scale dispersion of such compounds.
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- Hallikas, Outi K, et al.
(författare)
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Identification of Antibodies against HAI-1 and Integrin {alpha}6{beta}4 as
- 2006
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Ingår i: Journal of Histochemistry and Cytochemistry. - 0022-1554 .- 1551-5044. ; 54:7, s. 754-752
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Tidskriftsartikel (refereegranskat)abstract
- Syncytiotrophoblast and invasive extravillous trophoblast arise from a common stem cell, namely villous cytotrophoblast, but have very different characteristics. The study of the differentiation process relies on the availability of suitable markers for these different cell types of developing placenta. In this work, we have produced monoclonal antibodies that are specific to human villous cytotrophoblast. Monoclonal antibody (MAb) MG2 was specific to villous cytotrophoblast across gestation, and recognizes hepatocyte growth factor activator inhibitor type 1. MAb MD10 stained villous cytotrophoblast across gestation and also some endothelial cells, particularly in the second or third trimester. MAb MD10 recognizes human integrin α6β4. As a test for specificity, the novel MAbs were also used for staining of frozen tissue from human colon carcinoma. The results show that the two antibodies can be used as tools to study human villous cytotrophoblasts and also human tumors. The MG2 antibody seems most specific and promising for the study of various aspects of human villous cytotrophoblast.
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