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Sökning: WFRF:(Waldén Annelie)

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1.
  • Pei, Zhichao, et al. (författare)
  • Photogenerated Carbohydrate Microarrays
  • 2007
  • Ingår i: ChemBioChem (Print). - : Wiley-VCH Verlagsgesellschaft. - 1439-4227 .- 1439-7633. ; 8:2, s. 166-168
  • Tidskriftsartikel (refereegranskat)abstract
    • Chemical Equation Presented) Sugars in a row. A new strategy for carbohydrate microarrays based on photochemical ligation of perfluorophenylazide-derivatized carbohydrates to PEO surfaces is presented. It constitutes a controllable and robust method of array fabrication, on the carbohydrate chemistry and on the surface-chemistry levels, and the resulting carbohydrate arrays can be efficiently used to reveal the recognition patterns of carbohydrate-binding proteins.
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2.
  • Uhlén, Mathias, et al. (författare)
  • A human protein atlas for normal and cancer tissues based on antibody proteomics
  • 2005
  • Ingår i: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 4:12, s. 1920-1932
  • Tidskriftsartikel (refereegranskat)abstract
    • Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here we report a new publicly available database containing, in the first version, similar to 400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research.
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