| 1. |
- Wallmark, A, et al.
(författare)
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Feasibility of extracorporeal on-line large-scale plasma adsorptions on protein A-sepharose columns in cancer patients
- 1984
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Ingår i: Artificial Organs. - : Wiley. - 0160-564X .- 1525-1594. ; 8:1, s. 72-81
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Tidskriftsartikel (refereegranskat)abstract
- The feasibility of extracorporeal adsorption of 1.5-3 L plasma on protein A-Sepharose was investigated in six patients with advanced cancer. Anticoagulation with heparin was associated with respiratory distress syndrome in two patients, most likely caused by complement activation as indicated by a transient leukopenia during plasma reinfusion and appearance of C3 degradation products in the extracorporeal circulation. Addition of citrate abolished the respiratory symptoms, C3 degradation, and leukopenia, and no adverse reactions were observed. No objective tumor regression was observed in any of the patients. Three patients progressed during therapy. In one of these, multifocal central tumor necrosis was observed as a possible, although unproven, therapeutic effect. Increased natural killer and/or killer cell activities were recorded in three patients and increased complement-dependent serum cytotoxicity in one patient. The level of circulating immune complexes decreased significantly (18-28%) in three patients studied. It is concluded that extracorporeal plasma adsorption on protein A-Sepharose is feasible when citrate is added to the extracorporeal system, but its therapeutic efficacy is uncertain.
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| 2. |
- Brahme, A., et al.
(författare)
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Evaluation of a GEM and CAT-based detector for radiation therapy beam monitoring
- 2000
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Ingår i: Nuclear Instruments and Methods in Physics Research Section A. - 0168-9002 .- 1872-9576. ; 454, s. 136-141
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Tidskriftsartikel (refereegranskat)abstract
- We are developing a radiation therapy beam monitor for the Karolinska Institute. This monitor will consist of two consecutive detectors confined in one gas chamber: a keV-photon detector, which will allow diagnostic quality visualization of the patient, and a MeV-photon detector, that will measure the absolute intensity of the therapy beam and its position with respect to the patient. Both detectors are based on highly radiation resistant gas and solid photon to electron converters, combined with GEMs and a CAT as amplification structures. We have performed systematic studies of the high-rate characteristics of the GEM and the CAT, as well as tested the electron transfer through these electron multipliers and various types of converters. The tests show that the GEM and the CAT satisfy all requirements for the beam monitoring system. As a result of these studies we successfully developed and tested a full section of the beam monitor equipped with a MeV-photon converter placed between the GEM and the CAT.
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| 3. |
- Forsberg, C., et al.
(författare)
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Reference material for comparison of different adhesive tapes for forensic DNA sampling : -
- 2015
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Ingår i: Abstracts ISFG. ; , s. 267-268
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Konferensbidrag (refereegranskat)abstract
- Tape-lifting is an efficient method for collecting traces of cellular material from fabrics. Since 2006, an in-house adhesive tape has been used in casework at the Swedish National Forensic Centre, Linköping. Although this tape gives good DNA yields, we aim to replace it with a commercial tape to save cost and labor. In order to enable a fair comparison between different adhesive tapes, we have developed and evaluated a method for production of relevant reference material. One person, known to be a good shedder, wore identical long-sleeved T-shirts under controlled circumstances, and trace recovery was systematically performed with the in-house tape (3 T-shirts, total of 24 samples). Each sample was DNA extracted (Chelex) and quantified (Quantifiler Human DNA Quantification kit) to find the normal variation within the reference material.The DNA recovery differed considerably between samples, with obtained DNA concentrations between 0.010-0.481 ng/μL (mean: 0.083, standard deviation: 0.116 ng/μL). Applying such a reference material for comparison between two commercial tapes and our in-house tape resulted in mean DNA recoveries plus/ minus one standard deviation of 0.013±0.006 ng/μL (Scenesafe FAST Box), 0.012±0.007 ng/μL (Touch Tape), and 0.023±0.013 ng/μL (in-house tape).The in-house tape gave statistically significant higher yield compared to Touch Tape (p<0.05), but for Scenesafe the difference was not significant. Shedding of cells to worn clothes is a random process. Having a systematically prepared, casework-like reference material with known variation is therefore vital for comparative studies of tapes.
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| 4. |
- Forsberg, C., et al.
(författare)
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Reference material for comparison of different adhesive tapes for forensic DNA sampling
- 2015
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Ingår i: Forensic Science International: Genetics Supplement Series. - : Elsevier BV. - 1875-1768 .- 1875-175X. ; 5
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Tidskriftsartikel (refereegranskat)abstract
- Tape-lifting is an efficient method for collecting traces of cellular material from fabrics. Since 2006, an inhouse adhesive tape has been used in casework at the Swedish National Forensic Centre, Linkoping. Although this tape gives good DNA yields, we aim to replace it with a commercial tape to save cost and labour. In order to enable a fair comparison between different adhesive tapes, we have developed and evaluated a method for production of relevant reference material. One person, known to be a good shedder, wore identical long-sleeved T-shirts under controlled circumstances, and trace recovery was systematically performed with the in-house tape (3 T-shirts, total of 24 samples). Each sample was DNA extracted and quantified to find the normal variation within the reference material. The DNA recovery differed considerably between samples, with DNA concentrations between 0.010-0.48 ng/mu L (mean: 0.083, SD: 0.12 ng/mu L). Applying such a reference material for comparison between two commercial tapes and our in-house tape resulted in mean DNA recoveries plus/minus one standard deviation of 0.013 +/- 0.006 ng/mL (Scenesafe FAST Box), 0.012 +/- 0.007 ng/mu L (Touch tape), and 0.023 +/- 0.013 ng/mu L (inhouse tape). The in-house tape gave statistically significant higher yield compared to Touch tape (p < 0.05), but for Scenesafe FAST Box the difference was not significant. The shedding of cells to clothes cannot be fully controlled. Having a systematically prepared, casework-like reference material with known variation is therefore vital for comparative studies of tapes.
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| 5. |
- Larsson, M., et al.
(författare)
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Energy system analysis of the implications of hydrogen fuel cell vehicles in the Swedish road transport system
- 2014
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Ingår i: 20th World Hydrogen Energy Conference, WHEC 2014. - 9780000000002 ; , s. 2084-2091
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Konferensbidrag (refereegranskat)abstract
- The focus on pathways to reduce the use of fossil fuels in the transport sector is intense in many countries worldwide. Considering that biofuels have a limited technical production potential and that battery electric vehicles suffer from technical limitations that put constraints on their general use in the transport sector, hydrogen-fuelled fuel cell vehicles may become a feasible alternative. Introduction of hydrogen in the transport sector will also transform the energy sector and create new interactions. The aim of this paper is to analyse the consequences and feasibility of such an integration in Sweden. Different pathways for hydrogen, electricity and methane to the transport sector are compared with regard to system energy efficiency. The efficiencies for hydrogen and electricity are used for estimating the energy resources needed for hydrogen production and electric vehicles for a future Swedish transport sector based on renewable fuels. The analysis reveal that the well to wheel system efficiencies for hydrogen fuel cell vehicles are comparable to those of methane gas vehicles, even when methane gas is the primary energy source. The results further indicate that an increased hydrogen demand may have a less than expected impact on the primary energy supply in Sweden.
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| 6. |
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| 7. |
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| 8. |
- Peskov, V., et al.
(författare)
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The study and optimization of new micropattern gaseous detectors for high-rate applications
- 2001
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Ingår i: IEEE Transactions on Nuclear Science. - : Institute of Electrical and Electronics Engineers (IEEE). - 0018-9499 .- 1558-1578. ; 48:4, s. 1070-1074
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Tidskriftsartikel (refereegranskat)abstract
- We performed a new series of systematic studies of gain and rate characteristics of several micropattern gaseous detectors. Extending earlier studies, characteristics were measured at various pressures and gas mixtures at a wide range of primary charges, and also when the whole area of the detectors was irradiated with a high-intensity X-ray beam. Several new effects were discovered, common to all tested detectors, which define fundamental limits of operation. The results of these studies allow us to identify several concrete ways of improving the performance of micropattern detectors and to suggest that in some applications, resistive plate chambers may constitute a valid alternative. Being protected from damaging discharges by the resistive electrodes, these detectors feature high gain, high rate capability (10(5) Hz/mm(2)), good position resolution (better than 30 mum), and excellent timing (50 ps sigma).
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| 9. |
- Wallmark, M., et al.
(författare)
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Operating range of a gas electron multiplier for portal imaging
- 2001
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Ingår i: Nuclear Instruments and Methods in Physics Research Section A. - 0168-9002 .- 1872-9576. ; 471:02-jan, s. 151-155
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Tidskriftsartikel (refereegranskat)abstract
- At the Karolinska Institute in Stockholm, Sweden a new detector for portal imaging is under development, which could greatly improve the alignment of the radiation beam with respect to the tumor during radiation treatment. The detector is based on solid converters combined with gas electron multipliers (GEMs) as an amplification structure. The detector has a large area and will be operated in a very high rate environment in the presence of heavy ionizing particles. As was discovered recently high rates and alpha particles could cause discharges in GEM and discharge propagation from GEM to GEM and to the readout electronics. Since reliability is one of the main requirements for the portal imaging device, we performed systematic studies to find a safe operating range of the device, free from typical high rate problems, such as discharges.
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