| 1. |
- Janzi, Magdalena, et al.
(författare)
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Screening for C3 deficiency in newborns using microarrays.
- 2009
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Ingår i: PloS one. - : Public Library of Science (PLoS). - 1932-6203. ; 4:4, s. e5321-
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Dried blood spot samples (DBSS) from newborns are widely used in neonatal screening for selected metabolic diseases and diagnostic possibilities for additional disorders are continuously being evaluated. Primary immunodeficiency disorders comprise a group of more than one hundred diseases, several of which are fatal early in life. Yet, a majority of the patients are not diagnosed due to lack of high-throughput screening methods.METHODOLOGY/PRINCIPAL FINDINGS: We have previously developed a system using reverse phase protein microarrays for analysis of IgA levels in serum samples. In this study, we extended the applicability of the method to include determination of complement component C3 levels in eluates from DBSS collected at birth. Normal levels of C3 were readily detected in 269 DBSS from healthy newborns, while no C3 was detected in sera and DBSS from C3 deficient patients.CONCLUSIONS/SIGNIFICANCE: The findings suggest that patients with deficiencies of specific serum proteins can be identified by analysis of DBSS using reverse phase protein microarrays.
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| 2. |
- Janzi, Magdalena, et al.
(författare)
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Selective IgA deficiency in early life : Association to infections and allergic diseases during childhood
- 2009
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Ingår i: Clinical Immunology. - : Elsevier BV. - 1521-6616 .- 1521-7035. ; 133:1, s. 78-85
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Tidskriftsartikel (refereegranskat)abstract
- Selective IgA deficiency in early life is quite common in Caucasian populations, but it is unclear whether it increases the risk of infections and allergic diseases during childhood. Serum IgA levels were measured in 2423 children at 4 years of age in a Swedish population based birth cohort (BAMSE). Parental questionnaires were repeatedly sent out during the child's first 8 years of life, collecting information about infections and allergic diseases. 14 children (1:173) were found to be IgA deficient at 4 years of age. These children had an increased risk of pseudocroup at year 1 (p<0.01) and food hypersensitivity at year 4 (p<0.05) as compared to IgA sufficient children. No increased risk was observed in the partial IgA deficiency group. The findings suggest that selective IgA deficiency may increase the risk of parentally reported pseudocroup and food hypersensitivity during early childhood.
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| 3. |
- Uhlén, Mathias, et al.
(författare)
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A human protein atlas for normal and cancer tissues based on antibody proteomics
- 2005
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Ingår i: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 4:12, s. 1920-1932
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Tidskriftsartikel (refereegranskat)abstract
- Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here we report a new publicly available database containing, in the first version, similar to 400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research.
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| 4. |
- Zhao, Chunyan, et al.
(författare)
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Estrogen receptor beta 2 negatively regulates the transactivation of estrogen receptor alpha in human breast cancer cells
- 2007
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Ingår i: Cancer Research. - 0008-5472 .- 1538-7445. ; 67:8, s. 3955-3962
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Tidskriftsartikel (refereegranskat)abstract
- Estrogens, by binding to and activating two estrogen receptors (ER alpha and ER beta), are critically involved in the development of the mammary gland and breast cancer. An isoform of ER beta, ER beta 2 (also called ER beta cx), with an altered COOH-terminal region, is coexpressed with ER alpha. in many human breast cancers. In this study, we generated a stable cell line from MCF7 breast cancer cells expressing an inducible version of ER beta 2, along with endogenous ER alpha, and examined the effects of ER beta 2 on the ER alpha protein levels and function. We showed that ER beta 2 inhibited ER alpha-mediated transactivation via estrogen response element and activator protein-1 sites of reporter constructs as well as the endogenous genes pS2 and MMP-1. Chromatin immunoprecipitation assays revealed that ER beta 2 expression caused a significant reduction in the recruitment of ER alpha to both the pS2 and MMP-1 promoters. Furthermore, ER beta 2 expression induced proteasome-dependent degradation of ER alpha. The inhibitory effects of ER beta 2 on ER alpha activity were further confirmed in HEK293 cells that lack functional endogenous ER alpha. We also showed that ER beta 2 can interact with ER alpha both in vitro and in mammalian cells' which is compatible with a model where ER beta 2/ER alpha heterodimers are targeted to the proteasome. Finally, in human breast cancer samples, we observed that expression of ER beta 2 significantly correlated with ER alpha-negative phenotype. Our data suggest that ER beta 2 could influence ER alpha-mediated effects relevant for breast cancer development, including hormone responsiveness.
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