| 1. |
- Li, Shuqi, et al.
(författare)
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Rtt105 functions as a chaperone for replication protein A to preserve genome stability
- 2018
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Ingår i: EMBO Journal. - : Wiley-VCH Verlagsgesellschaft. - 0261-4189 .- 1460-2075. ; 37:17
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Tidskriftsartikel (refereegranskat)abstract
- Generation of single-stranded DNA (ssDNA) is required for the template strand formation during DNA replication. Replication Protein A (RPA) is an ssDNA-binding protein essential for protecting ssDNA at replication forks in eukaryotic cells. While significant progress has been made in characterizing the role of the RPA-ssDNA complex, how RPA is loaded at replication forks remains poorly explored. Here, we show that the Saccharomyces cerevisiae protein regulator of Ty1 transposition 105 (Rtt105) binds RPA and helps load it at replication forks. Cells lacking Rtt105 exhibit a dramatic reduction in RPA loading at replication forks, compromised DNA synthesis under replication stress, and increased genome instability. Mechanistically, we show that Rtt105 mediates the RPA-importin interaction and also promotes RPA binding to ssDNA directly in vitro, but is not present in the final RPA-ssDNA complex. Single-molecule studies reveal that Rtt105 affects the binding mode of RPA to ssDNA These results support a model in which Rtt105 functions as an RPA chaperone that escorts RPA to the nucleus and facilitates its loading onto ssDNA at replication forks.
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| 2. |
- Peng, Xiaofeng, et al.
(författare)
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Self-reconstruction of twisted Laguerre-Gaussian Schell-model beams partially blocked by an opaque obstacle
- 2020
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Ingår i: Optics Express. - : The Optical Society. - 1094-4087. ; 28:21, s. 31510-31523
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Tidskriftsartikel (refereegranskat)abstract
- Twisted Laguerre-Gaussian Schell model (TLGSM) beams are a novel type of partially coherent beams, which carry the twist phase and the vortex phase simultaneously. In this paper, the self-reconstruction (SR) of the TLGSM beam partially blocked by an opaque obstacle and propagating through a thin lens focusing system is studied in detail. Implementing the pseudo-mode expansion method, we represent the TLGSM beam as a superposition of spatially coherent modes, which is applicable for calculating the cross spectral density (CSD) and spectral degree of coherence (SDOC) of such a beam using the Fourier transform. The numerical results reveal that the twist parameter, a measure of the strength of a twist phase, determines the characteristics of the SR. In the case of the topological charge of the vortex is positive, the SR capability of spectral density is significantly improved with the increase of the twist parameter. The physical mechanism behind these phenomena is analyzed in detail from the point view of mode expansion. Further, the dependence of the side ring intensity and SR capability of the SDOC on the twist parameter are also investigated. It is found that the SR capability and side ring intensity of the SDOC are simultaneously enhanced for a large twist parameter, providing an efficient way to determine the number of topological charge. Our study results may find application in communication and information recovery applications.
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| 3. |
- Wang, Kang, et al.
(författare)
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Luminescent metal-halide perovskites: fundamentals, synthesis, and light-emitting devices
- 2024
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Ingår i: Science in China Series B. - : SCIENCE PRESS. - 1674-7291 .- 1869-1870. ; 67:6, s. 1776-1838
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Forskningsöversikt (refereegranskat)abstract
- Metal-halide perovskites have garnered considerable research attention as highly efficient light emitters in recent years due to their outstanding optoelectronic properties with remarkable tunability and excellent solution processabilities. Substantial advancements have been achieved in the development of novel halide perovskites, and the exploitations of these materials in light-emitting devices. This review comprehensively outlines recent breakthroughs in metal-halide perovskites, encompassing the rational design of perovskite materials with tunable light emission properties, the controllable growth of single crystal for a deeper understanding of their structure-property relationships, as well as the fundamental insights into the photophysics and carrier dynamics in perovskite systems. Additionally, it provides an overview of recent applications of perovskite materials in high-performance light-emitting diodes (LEDs) and lasers.
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