| 1. |
- Wang, Huan, et al.
(författare)
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Boosting the Electrochemical Performance of PI-5-CA/C-SWCNT Nanohybrid for Sensitive Detection of E. coli O157:H7 From the Real Sample
- 2022
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Ingår i: Frontiers in Chemistry. - : Frontiers Media SA. - 2296-2646. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- Redox activity is an important indicator for evaluating electrochemical biosensors. In this work, we have successfully polymerized indole-5-carboxylic acid into poly-5-carboxyindole nanomaterials (PI-5-CA), using its superior redox activity, and introduced carboxylated single-walled carbon nanotubes (C-SWCNTs) to synthesize a composite material. Finally, a synthesized composite material was used for the modification of the glass carbon electrode to fabricate the PI-5-CA/C-SWCNTs/GCE-based immunosensor and was successfully applied for the sensitive detection of E. coli O157:H7. The fabricated immunosensor exhibited an outstanding electrocatalytic activity toward the detection of E. coli O157:H7 with a remarkably lowest limit of detection (2.5 CFU/ml, LOD = 3 SD/k, n = 3) and has a wide linear range from 2.98x10(1) to 2.98x10(7) CFU/ml. Inspired from the excellent results, the fabricated electrode was applied for the detection of bacteria from real samples (water samples) with a good recovery rate (98.13-107.69%) as well as an excellent stability and specificity. Owing to its simple preparation, excellent performance, and detection time within 30 min, our proposed immunosensor will open a new horizon in different fields for the sensitive detection of bacteria from real samples.
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| 2. |
- Li, Songjun, et al.
(författare)
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‘On/off’-switchable catalysis by a smart enzyme-like imprinted polymer
- 2011
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Ingår i: Journal of Catalysis. - : Elsevier. - 0021-9517 .- 1090-2694. ; 278:2, s. 173-180
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Tidskriftsartikel (refereegranskat)abstract
- ‘On/off’-switchable catalysis by a smart enzyme-like imprinted polymer is reported. This unique imprinted polymer was composed of poly(N-isopropylacrylamide)-containing p-nitrophenyl phosphate-imprinted networks that exhibited temperature-dependent hydrophilicity/hydrophobicity. At a relatively low temperature (such as 20 °C), this polymer was capable of vigorous catalysis for the hydrolysis of p-nitrophenyl acetate due to its hydrophilic networks, which enabled access to the imprinted framework. On the contrary, at higher temperatures (such as 40 °C), this polymer demonstrated poor catalysis resulting from its dramatically increased hydrophobicity, which inhibited access to the imprinted sites. Unlike previously reported imprinted polymers which lack adjustable networks, this novel imprinted polymer employed thermosensitive poly(N-isopropylacrylamide) networks, thus enabling the switchable catalysis.
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| 3. |
- Wang, Shenqi, et al.
(författare)
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Detection of [Ca2+]i Changes in Sub-plasma Membrane Microdomains in a Single Living Cell by an Optical Fiber-based Nanobiosensor
- 2014
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Ingår i: Austin Journal of Nanomedicine and Nanotechnology. - 2381-8956. ; 2:4, s. 1022-
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Tidskriftsartikel (refereegranskat)abstract
- n optical fiber-based nanobiosensor, for advanced detection of [Ca2+]i (i.e. intracellular Ca2+ concentration) changes in sub–plasma membrane microdomains in a single living smooth muscle cell and a single living cardiomyocyte, was successfully prepared by coating silver and then immobilizing Calcium Green–1 Dextran, a calcium ion sensitive dye, on the distal end of the nanoprobe. The constructed nanobiosensor was capable of detecting ultra–low and local intracellular calcium ion concentration within the nanomolar range, which is around the physiological level of free cytosolic calcium ion in a single living cell. The response time was less than milliseconds enabling the detection of transient elementary calcium ion signaling events associated with calcium ion microdomains. The effects of stimulants such as high potassium buffer solution and norepinephrine solution were also investigated. The resulting system could thus greatly facilitate the development of an advanced nano–diagnostic platform for in vivo and real–time sensing/diagnosing of [Ca2+]i at the single cell level.
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