| 1. |
- Chester, Alan, et al.
(author)
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Alpha-L-fucosyltransferases in human submaxillary gland and stomach tissues associated with the H, Lea and Leb blood-group characters and ABH secretor status
- 1969
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In: Biochemical and Biophysical Research Communications. - 1090-2104. ; 34:6, s. 835-842
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Journal article (peer-reviewed)abstract
- Schemes for the biosynthetic steps in the formation of the blood group active glycoproteins in secretions postulate that α-Image -fucosyltransferases specified by the Image and Image genes control the addition of Image -fucose to different acceptor sites in a precursor substance to give H, Lea and Leb serologically active structures. 1,2. The presence of 2-, 3- and 4-α-Image -fucosyl-transferases in submaxillary glands and stomach mucosal linings from persons who are secretors of ABH, and of 3- and 4-α-Image -fucosyltransferases in tissues from non-secretor persons, is described in this paper.
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| 2. |
- Chester, Alan, et al.
(author)
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Phenyl beta-D-Galactopyranoside as an Acceptor Substrate for the Blood-Group H Gene-Associated Guanosine Diphosphate L-Fucose:beta-D-Galactosyl alpha-2-L-Fucosyltransferase
- 1976
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In: European Journal of Biochemistry. - 0014-2956. ; 69:2, s. 583-592
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Journal article (peer-reviewed)abstract
- Phenyl β-d-galactopyranoside was found to be an efficient acceptor of l-[14C]fucose when guanosine diphosphate l-[14C]fucose was used as the donor substrate and human serum, submaxillary glands or stomach mucosa were the sources of l-fucosyltransferase. The enzyme utilising phenyl β-D-galactoside was present in the serum of donors of all ABO blood-groups examined, except those of the rare Oh (Bombay) and Bh phenotypes, but was clearly demonstrable in submaxillary gland preparations only when the glands came from individuals who were secretors of ABH blood-group substances. This distribution coincides with that previously established for the blood-group H gene-specified α-2-l-fucosyltransferase. The product of l-[14C]fucosyl transfer to phenyl β-d-galactoside is separable from the other radioactive components in the enzyme digest by chromatography for 4 h in ethyl acetate/pyridine/water (10/4/3, by vol.); it was characterised as phenyl 2-O-(α-l-[14C]fucopyranosyl)β-d-galactopyranoside by (a) the liberation of l-[14C]fucose by a specific α-2-l-fucosidase, (b) its resistance to degradation by alkali and (c) the identification of tritiated glycerol as a product of periodate oxidation after a 3H label had been introduced into the galactosyl moiety. The use of phenyl β-d-galactopyranoside as acceptor substrate thus provides a simple and relatively rapid method for the assay of the blood-group H gene-specified α-2-l-fucosyltransferase.
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