| 1. |
- Brebu, Mihai, et al.
(författare)
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Putative volatile biomarkers of bovine tuberculosis infection in breath, skin and feces of cattle
- 2023
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Ingår i: Molecular and Cellular Biochemistry. - : Springer. - 0300-8177 .- 1573-4919. ; 478:11, s. 2473-2480
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Tidskriftsartikel (refereegranskat)abstract
- Bovine tuberculosis (bTB) is an infectious disease with significant impact on animal health, public health and international trade. Standard bTB screening in live cattle consists in injecting tuberculin and measuring the swelling at the place of injection few days later. This procedure is expensive, time-consuming, logistically challenging, and is not conclusive before performing confirmatory tests and additional analysis. The analysis of the volatile organic compounds (VOCs) emitted by non-invasive biological samples can provide an alternative diagnostic approach suitable for bTB screening. In the present study, we analyzed VOC samples emitted through the breath, feces and skin of 18 cows diagnosed with bTB from three farms from Romania, as well as of 27 negative cows for bTB from the same farms. Analytical studies employing gas chromatography coupled to mass spectrometry revealed 80 VOCs emitted through the breath, 200 VOCs released by feces, and 80 VOCs emitted through the skin. Statistical analysis of these compounds allowed the identification of 3 tentative breath VOC biomarkers (acetone; 4-methyldecane; D-limonene), 9 tentative feces VOC biomarkers (toluene; [(1,1-dimethylethyl)thio]acetic acid; alpha-thujene; camphene; phenol; o-cymene; 3-(1,1-dimethylethyl)-2,2,4,4-tetramethyl-3-pentanol; 2,5-dimethylhexane-2,5-dihydroperoxide; 2,4-di-tert-butylphenol), and 3 tentative skin VOC biomarkers (ammonia; 1-methoxy-2-propanol; toluene). The possible pathway of these volatile biomarkers is discussed.
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| 2. |
- de Jesus Beleno-Saenz, Kelvin, et al.
(författare)
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Non-Invasive Method to Detect Infection with Mycobacterium tuberculosis Complex in Wild Boar by Measurement of Volatile Organic Compounds Obtained from Feces with an Electronic Nose System
- 2021
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Ingår i: Sensors. - : MDPI. - 1424-8220. ; 21:2
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Tidskriftsartikel (refereegranskat)abstract
- More effective methods to detect bovine tuberculosis, caused by Mycobacterium bovis, in wildlife, is of paramount importance for preventing disease spread to other wild animals, livestock, and human beings. In this study, we analyzed the volatile organic compounds emitted by fecal samples collected from free-ranging wild boar captured in Donana National Park, Spain, with an electronic nose system based on organically-functionalized gold nanoparticles. The animals were separated by the age group for performing the analysis. Adult (>24 months) and sub-adult (12-24 months) animals were anesthetized before sample collection, whereas the juvenile (<12 months) animals were manually restrained while collecting the sample. Good accuracy was obtained for the adult and sub-adult classification models: 100% during the training phase and 88.9% during the testing phase for the adult animals, and 100% during both the training and testing phase for the sub-adult animals, respectively. The results obtained could be important for the further development of a non-invasive and less expensive detection method of bovine tuberculosis in wildlife populations.
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| 3. |
- Drozdowska, Katarzyna, et al.
(författare)
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Combined chemoresistive and in situ FTIR spectroscopy study of nanoporous NiO films for light-activated nitrogen dioxide and acetone gas sensing
- 2022
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Ingår i: Sensors and actuators. B, Chemical. - : Elsevier. - 0925-4005 .- 1873-3077. ; 353
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Tidskriftsartikel (refereegranskat)abstract
- The chemoresistive sensor response of nanoporous NiO films prepared by advanced gas deposition was investigated by combined resistivity and in situ FTIR spectroscopy, with and without simultaneous light illumination, to detect NO2 and acetone gases. The sensitivity towards NO2 increased dramatically under UV irradiation employing 275 nm light. Improved sensitivity was observed at an elevated temperature of 150 degrees C. In situ FTIR measurements were performed to record the transient gas adsorption/desorption processes. The sustained sensitivity and repeatability for NO2 sensing could be attributed to reversible surface-nitro and nitrate species formation, which are stable on the surface at relative humidity up to 40%. In contrast, acetone sensing results in irreversible decomposition and accumulation of reaction products on the NiO sensor surface, covering the surface and limiting gas sensing. Implications of the study for improved and sustained NiO gas sensor properties in gas mixtures are discussed.
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| 4. |
- Durán-Acevedo, Cristhian Manuel, et al.
(författare)
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Exhaled breath analysis for gastric cancer diagnosis in Colombian patients.
- 2018
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Ingår i: Oncotarget. - : Impact Journals, LLC. - 1949-2553. ; 9:48, s. 28805-28817
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Tidskriftsartikel (refereegranskat)abstract
- We present here the first study that directly correlates gastric cancer (GC) with specific biomarkers in the exhaled breath composition on a South American population, which registers one of the highest global incidence rates of gastric affections. Moreover, we demonstrate a novel solid state sensor that predicts correct GC diagnosis with 97% accuracy. Alveolar breath samples of 30 volunteers (patients diagnosed with gastric cancer and a controls group formed of patients diagnosed with other gastric diseases) were collected and analyzed by gas-chromatography/mass-spectrometry (GC-MS) and with an innovative chemical gas sensor based on gold nanoparticles (AuNP) functionalized with octadecylamine ligands. Our GC-MS analyses identified 6 volatile organic compounds that showed statistically significant differences between the cancer patients and the controls group. These compounds were different from those identified in previous studied performed on other populations with high incidence rates of this malady, such as China (representative for Eastern Asia region) and Latvia (representative for Baltic States), attributable to lifestyle, alimentation and genetics differences. A classification model based on principal component analysis of our sensor data responses to the breath samples yielded 97% accuracy, 100% sensitivity and 93% specificity. Our results suggest a new and non-intrusive methodology for early diagnosis of gastric cancer that may be deployed in regions lacking well-developed health care systems as a prediagnosis test for selecting the patients that should undergo deeper investigations (e.g., endoscopy and biopsy).
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| 5. |
- Geremariam Welearegay, Tesfalem, et al.
(författare)
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Diagnosis of Human Echinococcosis via Exhaled Breath Analysis : A Promise for Rapid Diagnosis of Infectious Diseases Caused by Helminths
- 2019
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Ingår i: Journal of Infectious Diseases. - : Oxford University Press (OUP). - 0022-1899 .- 1537-6613. ; 219:1, s. 101-109
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Tidskriftsartikel (refereegranskat)abstract
- Background: Human echinococcosis is a neglected infectious disease affecting more than 1 million people globally. Its diagnosis is expensive and difficult because of lack of adequate resources in low-resource locations, where most cases occur.Methods: A group of volunteers diagnosed with the 2 main types of echinococcosis and corresponding control groups were recruited from hospitals in Tunisia (32 patients with cystic echinococcosis and 43 controls) and Poland (16 patients with alveolar echinococcosis and 8 controls). Breath samples were collected from all patients and analyzed by gas chromatography coupled to mass spectrometry, and a specifically developed electronic nose system.Results: The chemical analysis revealed statistically different concentrations of 2 compounds in the breath of patients with cystic echinococcosis compared to controls, and statistically different concentrations of 7 compounds in the breath of patients with alveolar echinococcosis compared to controls. The discrimination accuracy achieved by the electronic nose system was 100% for cystic echinococcosis and 92.9% for alveolar echinococcosis, while the discrimination accuracy between these 2 patient groups was 92.1%.Conclusion: Here we advocate a noninvasive, fast, easy-to-operate and nonexpensive diagnostic tool for the diagnosis of human echinococcosis disease through exhaled breath analysis, suitable for early diagnosis and population screening.
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| 6. |
- Geremariam Welearegay, Tesfalem, et al.
(författare)
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Fabrication and characterisation of ligand-functionalised ultrapure monodispersed metal nanoparticle nanoassemblies employing advanced gas deposition technique
- 2018
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Ingår i: Nanotechnology. - : IOP PUBLISHING LTD. - 0957-4484 .- 1361-6528. ; 29:6
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Tidskriftsartikel (refereegranskat)abstract
- Here, we report for the first time the fabrication of ligand-functionalised ultrapure monodispersed metal nanoparticles (Au, Cu, and Pt) from their pure metal precursors using the advanced gas deposition technique. The experimental conditions during nanoparticle formation were adjusted in order to obtain ultrafine isolated nanoparticles on different substrates. The morphology and surface analysis of the as-deposited metal nanoparticles were investigated using scanning electron microscopy, x-ray diffraction and Fourier transform infra-red spectroscopy, which demonstrated the formation of highly ordered pure crystalline nanoparticles with a relatively uniform size distribution of similar to 10 nm (Au), similar to 4 nm (Cu) and similar to 3 nm (Pt), respectively. A broad range of organic ligands containing thiol or amine functional groups were attached to the nanoparticles to form continuous networks of nanoparticle-ligand nanoassemblies, which were characterised by scanning electron microscopy and x-ray photoelectron spectroscopy. The electrical resistance of the functional nanoassemblies deposited in the gap spacing of two microfabricated parallel Au electrodes patterned on silicon substrates ranged between tens of k Omega and tens of M Omega, which is suitable for use in many applications including (bio)chemical sensors, surface-enhanced Raman spectroscopy and molecular electronic rectifiers.
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| 7. |
- Ionescu, Radu, et al.
(författare)
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Fabrication of ultra-pure gold nanoparticles capped with dodecanethiol for Schottky-diode chemical gas sensing devices
- 2017
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Ingår i: Sensors and actuators. B, Chemical. - : Elsevier BV. - 0925-4005 .- 1873-3077. ; 239, s. 455-461
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Tidskriftsartikel (refereegranskat)abstract
- Ultra-pure monolayer-capped gold nanoparticles for chemical gas sensing devices were prepared by a novel two-step process: a physical vapour deposition technique was first employed to make dispersed ultra-pure size-controlled gold nanoparticles, and this step was followed by a coating process for functionalization of the gold nanoparticles with an organic ligand, specifically dodecanethiol. X-ray photoelectron spectroscopy proved that the nano-assemblies had high purity. Chemical sensing devices based on these nano-assemblies showed Schottky-diode behaviour. We believe this is the first observation of Schottky-diodes fabricated from nanomaterials based on metallic nanoparticles. Gas sensing experiments demonstrated that these devices were suitable for detecting volatile organic compounds.
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| 8. |
- Jaimes-Mogollon, Aylen Lisset, et al.
(författare)
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Review on Volatolomic Studies as a Frontier Approach in Animal Research
- 2021
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Ingår i: Advanced Biology. - : John Wiley & Sons. - 2701-0198. ; 5:6
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Forskningsöversikt (refereegranskat)abstract
- This paper presents a comprehensive review of the research studies in volatolomics performed on animals so far. At first, the procedures proposed for the collection, preconcentration, and storing of the volatile organic compounds emitted by various biological samples of different animals are presented and discussed. Next, the results obtained in the analysis of the collected volatile samples with analytical equipment are shown. The possible volatile biomarkers identified for various diseases are highlighted for different types of diseases, animal species, and biological samples analyzed. The chemical classes of these compounds, as well as the biomarkers found in a higher number of animal diseases, are indicated, and their possible origin is analyzed. The studies that dealt with the diagnosis of various diseases from sample measurement with electronic nose systems are also presented and discussed. The paper ends with a final remark regarding the necessity of optimization and standardization of sample collection and analysis procedures for obtaining meaningful results.
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| 9. |
- Kwiatkowski, Andrzej, et al.
(författare)
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Assessment of Electronic Sensing Techniques for the Rapid Identification of Alveolar Echinococcosis through Exhaled Breath Analysis
- 2020
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Ingår i: Sensors. - : MDPI AG. - 1424-8220. ; 20:9
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Tidskriftsartikel (refereegranskat)abstract
- Here we present a proof-of-concept study showing the potential of a chemical gas sensors system to identify the patients with alveolar echinococcosis disease through exhaled breath analysis. The sensors system employed comprised an array of three commercial gas sensors and a custom gas sensor based on WO3 nanowires doped with gold nanoparticles, optimized for the measurement of common breath volatile organic compounds. The measurement setup was designed for the concomitant measurement of both sensors DC resistance and AC fluctuations during breath samples exposure. Discriminant Function Analysis classification models were built with features extracted from sensors responses, and the discrimination of alveolar echinococcosis was estimated through bootstrap validation. The commercial sensor that detects gases such as alkane derivatives and ethanol, associated with lipid peroxidation and intestinal gut flora, provided the best classification (63.4% success rate, 66.3% sensitivity and 54.6% specificity) when sensors’ responses were individually analyzed, while the model built with the AC features extracted from the responses of the cross-reactive sensors array yielded 90.2% classification success rate, 93.6% sensitivity and 79.4% specificity. This result paves the way for the development of a noninvasive, easy to use, fast and inexpensive diagnostic test for alveolar echinococcosis diagnosis at an early stage, when curative treatment can be applied to the patients.
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| 10. |
- Kwiatkowski, Andrzej, et al.
(författare)
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Enhanced visible light-activated gas sensing properties of nanoporous copper oxide thin films
- 2024
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Ingår i: Solar Energy Materials and Solar Cells. - : Elsevier. - 0927-0248 .- 1879-3398. ; 273
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Tidskriftsartikel (refereegranskat)abstract
- Metal oxide gas sensors are popular chemoresistive sensors. They are used for numerous tasks, including environmental and safety monitoring. Some gas-sensing materials exhibit photo-induced properties that can be utilized for enhanced gas detection by modifying the sensor selectivity and sensitivity when illuminated by light. Here, we present the gas sensing characteristics of highly nanoporous Cu2O thin films towards both electrophilic (NO2) and nucleophilic (C2H5OH, NH3) gas molecules under ambient temperature and modulated by visible light illumination of different colors (red: 632 nm, green: 530 nm, blue: 468 nm). Cu2O films were fabricated by reactive advanced gas deposition (AGD) technology. The surface and structural analysis of the samples confirm the deposition of nanoporous thin films of mixed copper oxide phases. The gas sensing property of Cu2O exhibited expected p-type semiconductor behavior upon electrophilic and nucleophilic gas exposures. Our results show that visible light illumination provides enhanced sensor response.
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