| 1. |
- Blasiak, Robert, et al.
(författare)
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Promoting diversity and inclusiveness in seafood certification and ecolabelling : Prospects for Asia
- 2017
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Ingår i: Marine Policy. - : Elsevier BV. - 0308-597X .- 1872-9460. ; 85, s. 42-47
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Tidskriftsartikel (refereegranskat)abstract
- Building on the inputs by a range of experts who participated in the February 2017 international symposium on Designing the Future for Fisheries Certification Schemes at the University of Tokyo, this manuscript traces the origins of fisheries certification schemes, relevant developments, and remaining challenges from an Asian perspective. Over the past 20 years, seafood certification has emerged as a powerful tool for meeting growing demands for sustainable fisheries and aquaculture products. Despite broad consensus among countries regarding what constitute responsible fishing practices, the fisheries certification landscape remains uneven. A plethora of certification schemes has generated confusion among consumers and retailers, and capital-intensive certification schemes may be out-of-reach or impractical for some small-scale fisheries, particularly within the developing world. A recent initiative by the Global Sustainable Seafood Initiative (GSSI) is aiming to address the diversity within the certification landscape by creating a tool to benchmark certification schemes that are in line with the FAO Code of Conduct for Responsible Fisheries and other relevant agreed FAO guidelines on fisheries, ecolabelling and aquaculture. Countries in Asia are among the world's top consumers and exporters of seafood, yet have faced some particular challenges with regard to seafood certification, underscoring the need for certification schemes that account for regional and local conditions and management practices, particularly with regard to small-scale fisheries.
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| 2. |
- Marques, André R A, et al.
(författare)
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Glucosylated cholesterol in mammalian cells and tissues: formation and degradation by multiple cellular β-glucosidases.
- 2016
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Ingår i: Journal of Lipid Research. - 1539-7262. ; 57, s. 451-463
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Tidskriftsartikel (refereegranskat)abstract
- The membrane lipid glucosylceramide (GlcCer) is continuously formed and degraded. Cells express two GlcCer-degrading β-glucosidases, GBA and GBA2, located in and outside the lysosome, respectively. Here we demonstrate that through transglucosylation both GBA and GBA2 are able to catalyze in vitro the transfer of glucosyl-moieties from GlcCer to cholesterol, and vice versa. Furthermore, the natural occurrence of 1-O-cholesteryl-β-D-glucopyranoside (GlcChol) in mouse tissues and human plasma is demonstrated using LC-MS/MS and 13C6-labelled GlcChol as internal standard. In cells the inhibition of GBA increases GlcChol, whereas inhibition of GBA2 decreases glucosylated sterol. Similarly, in GBA2-deficient mice GlcChol is reduced. Depletion of GlcCer by inhibition of GlcCer synthase decreases GlcChol in cells and likewise in plasma of inhibitor-treated Gaucher disease patients. In tissues of mice with Niemann-Pick type C, a condition characterized by intralysosomal accumulation of cholesterol, marked elevations in GlcChol occur as well. When lysosomal accumulation of cholesterol is induced in cultured cells, GlcChol is formed via lysosomal GBA. This illustrates that reversible transglucosylation reactions are highly dependent on local availability of suitable acceptors. In conclusion, mammalian tissues contain GlcChol formed by transglucosylation through β-glucosidases using GlcCer as donor. Our findings reveal a novel metabolic function for GlcCer.
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