| 1. |
|
|
| 2. |
- Reiner, A. T., et al.
(författare)
-
Concise Review: Developing Best-Practice Models for the Therapeutic Use of Extracellular Vesicles
- 2017
-
Ingår i: Stem Cells Translational Medicine. - : Oxford University Press (OUP). - 2157-6564 .- 2157-6580. ; 6:8
-
Forskningsöversikt (refereegranskat)abstract
- Growing interest in extracellular vesicles (EVs, including exosomes and microvesicles) as therapeutic entities, particularly in stem cell-related approaches, has underlined the need for standardization and coordination of development efforts. Members of the International Society for Extracellular Vesicles and the Society for Clinical Research and Translation of Extracellular Vesicles Singapore convened a Workshop on this topic to discuss the opportunities and challenges associated with development of EV-based therapeutics at the preclinical and clinical levels. This review outlines topic-specific action items that, if addressed, will enhance the development of best-practice models for EV therapies.
|
|
| 3. |
- Börger, V., et al.
(författare)
-
International Society for Extracellular Vesicles and International Society for Cell and Gene Therapy statement on extracellular vesicles from mesenchymal stromal cells and other cells: considerations for potential therapeutic agents to suppress coronavirus disease-19
- 2020
-
Ingår i: Cytotherapy. - : Elsevier BV. - 1465-3249. ; 22:9, s. 482-485
-
Tidskriftsartikel (refereegranskat)abstract
- STATEMENT: The International Society for Cellular and Gene Therapies (ISCT) and the International Society for Extracellular Vesicles (ISEV) recognize the potential of extracellular vesicles (EVs, including exosomes) from mesenchymal stromal cells (MSCs) and possibly other cell sources as treatments for COVID-19. Research and trials in this area are encouraged. However, ISEV and ISCT do not currently endorse the use of EVs or exosomes for any purpose in COVID-19, including but not limited to reducing cytokine storm, exerting regenerative effects or delivering drugs, pending the generation of appropriate manufacturing and quality control provisions, pre-clinical safety and efficacy data, rational clinical trial design and proper regulatory oversight. © 2020 International Society for Cell and Gene Therapy
|
|
| 4. |
|
|
| 5. |
- Clayton, A., et al.
(författare)
-
Considerations towards a roadmap for collection, handling and storage of blood extracellular vesicles
- 2019
-
Ingår i: Journal of Extracellular Vesicles. - : Wiley. - 2001-3078. ; 8:1
-
Tidskriftsartikel (refereegranskat)abstract
- There is an increasing interest in exploring clinically relevant information that is present in body fluids, and extracellular vesicles (EVs) are intrinsic components of body fluids ("liquid biopsies"). In this report, we will focus on blood. Blood contains not only EVs but also cells, and non-EV particles including lipoproteins. Due to the high concentration of soluble proteins and lipoproteins, blood, plasma and serum have a high viscosity and density, which hampers the concentration, isolation and detection of EVs. Because most if not all studies on EVs are single-centre studies, their clinical relevance remains limited. Therefore, there is an urgent need to improve standardization and reproducibility of EV research. As a first step, the International Society on Extracellular Vesicles organized a biomarker workshop in Birmingham (UK) in November 2017, and during that workshop several working groups were created to focus on a particular body fluid. This report is the first output of the blood EV work group and is based on responses by work group members to a questionnaire in order to discover the contours of a roadmap. From the answers it is clear that most respondents are in favour of evidence-based research, education, quality control procedures, and physical models to improve our understanding and comparison of concentration, isolation and detection methods. Since blood is such a complex body fluid, we assume that the outcome of the survey may also be valuable for exploring body fluids other than blood.
|
|
| 6. |
- Couch, Y., et al.
(författare)
-
A brief history of nearly EV-erything - The rise and rise of extracellular vesicles
- 2021
-
Ingår i: Journal of Extracellular Vesicles. - : Wiley. - 2001-3078. ; 10:14
-
Tidskriftsartikel (refereegranskat)abstract
- Extracellular vesicles (EVs) are small cargo-bearing vesicles released by cells into the extracellular space. The field of EVs has grown exponentially over the past two decades; this growth follows the realisation that EVs are not simply a waste disposal system as had originally been suggested by some, but also a complex cell-to-cell communication mechanism. Indeed, EVs have been shown to transfer functional cargo between cells and can influence several biological processes. These small biological particles are also deregulated in disease. As we approach the 75th anniversary of the first experiments in which EVs were unknowingly isolated, it seems right to take stock and look back on how the field started, and has since exploded into its current state. Here we review the early experiments, summarise key findings that have propelled the field, describe the growth of an organised EV community, discuss the current state of the field, and identify key challenges that need to be addressed.
|
|
| 7. |
- Hendrix, A., et al.
(författare)
-
Extracellular vesicle analysis
- 2023
-
Ingår i: Nature Reviews Methods Primers. - 2662-8449. ; 3:1
-
Forskningsöversikt (refereegranskat)abstract
- Cells release small, phospholipid membrane-enclosed particles, collectively referred to as extracellular vesicles (EVs), into their surroundings to enable intercellular communication. EVs have numerous functions in physiological and pathophysiological processes and show considerable promise for diagnostic and therapeutic applications. Technologies have rapidly evolved over the past two decades, providing a powerful, versatile toolset for preparing and characterizing EVs to facilitate research and translational efforts. However, considering the plethora of methods available, it is challenging to understand what makes one method more suited for a given experiment than another. The heterogeneity of EVs as well as the diversity in composition of their surroundings further add to this challenge. This Primer provides guidance for EV analysis across ecosystems, including accessible body- and environment-derived sources. We summarize the multi-step process of EV preparation, cover the guiding principles and considerations when performing and interpreting EV experiments, and reflect on the limitations and challenges in the fields of fundamental biology, biomarker development and therapeutic strategies. Extracellular vesicles (EVs) have vital functions and promise diagnostic and therapeutic applications. In this Primer, Hendrix and colleagues discuss tools for isolating and characterizing EVs obtained from various sources, including the body and the environment.
|
|
| 8. |
- Soekmadji, C., et al.
(författare)
-
Towards mechanisms and standardization in extracellular vesicle and extracellular RNA studies: results of a worldwide survey
- 2018
-
Ingår i: Journal of Extracellular Vesicles. - : Wiley. - 2001-3078. ; 7:1
-
Tidskriftsartikel (refereegranskat)abstract
- The discovery that extracellular vesicles (EVs) can transfer functional extracellular RNAs (exRNAs) between cells opened new avenues into the study of EVs in health and disease. Growing interest in EV RNAs and other forms of exRNA has given rise to research programmes including but not limited to the Extracellular RNA Communication Consortium (ERCC) of the US National Institutes of Health. In 2017, the International Society for Extracellular Vesicles (ISEV) administered a survey focusing on EVs and exRNA to canvass-related views and perceived needs of the EV research community. Here, we report the results of this survey. Overall, respondents emphasized opportunities for technical developments, unraveling of molecular mechanisms and standardization of methodologies to increase understanding of the important roles of exRNAs in the broader context of EV science. In conclusion, although exRNA biology is a relatively recent emphasis in the EV field, it has driven considerable interest and resource commitment. The ISEV community looks forward to continuing developments in the science of exRNA and EVs, but without excluding other important molecular constituents of EVs.
|
|
