| 1. |
- Czub, Joanna, et al.
(författare)
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Biological effects of mixed-ion beams. Part 1 : Effect of irradiation of the CHO-K1 cells with a mixed-ion beam containing the carbon and oxygen ions
- 2018
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Ingår i: Applied Radiation and Isotopes. - : Elsevier BV. - 0969-8043 .- 1872-9800. ; 139, s. 304-309
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Tidskriftsartikel (refereegranskat)abstract
- Carbon and oxygen ions were accelerated simultaneously to estimate the effect of irradiation of living cells with the two different ions. This mixed ion beam was used to irradiate the CHO-K1 cells, and a survival test was performed. The type of the effect of the mixed ion beam on the cells was determined with the isobologram method, whereby survival curves for irradiations with individual ion beams were also used. An additive effect of irradiation with the two ions was found.
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| 2. |
- Czub, Joanna, et al.
(författare)
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Biological effects of mixed-ion beams. Part 2 : The relative biological effectiveness of CHO-K1 cells irradiated by mixed- and single-ion beams
- 2019
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Ingår i: Applied Radiation and Isotopes. - : Elsevier BV. - 0969-8043 .- 1872-9800. ; 150, s. 192-198
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Tidskriftsartikel (refereegranskat)abstract
- The relative biological effectiveness (RBE) values were determined for single- and mixed-ion beams containing carbon and oxygen ions. The CHO-K1 cells were irradiated with beams with the linear energy transfer (LET) values of 236-300 and 461-470 keV/mu m for C-12 and O-16 ions, respectively. The RBE was estimated as a function of dose, survival fraction (SF) and LET. The SF was not affected by varying contributions of the constituent ions to the total mixed dose. The RBE has the same value for single-ion exposures with ions with LET 300 (C-12) and 470 keV/mu m (O-16).
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| 3. |
- Jucha, Anna, et al.
(författare)
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FociCounter : A freely available PC programme for quantitative and qualitative analysis of gamma-H2AX foci
- 2010
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Ingår i: Mutation research. - : Elsevier BV. - 0027-5107 .- 1873-135X. ; 696:1, s. 16-20
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Tidskriftsartikel (refereegranskat)abstract
- Gamma-H2AX foci are sensitive and specific indicator for the induction of DNA double-strand breaks (DSBs) and an immunocytochemical assay with antibodies recognizing gamma-H2AX has become the gold standard for the detection of this type of DNA lesion. Quantification of gamma-H2AX foci can be achieved by various methods such as Western blotting, flow cytometry, visual analysis and computational analysis with a fluorescence microscope. The best sensitivity is achieved by computer analysis. Since no freeware programme for the analysis of gamma-H2AX foci exists for a PC platform, the aim of our study was to develop a simple and user-friendly public-domain software. The algorithm applied in our programme allows determination of the number of foci in a single cell, a focus intensity per cell, as well as a cell intensity. Its graphical user interface is based on a GTK+ library and the whole application can be run under a variety of operating systems, including MS Windows and Linux. The programme called FociCounter is publicly available at http://focicounter.sourceforge.net. Application of the programme was tested by analysing gamma-H2AX foci in CHO and MO59K cells irradiated in vitro with X-rays and validated by comparing the results obtained with the outcome of automated image analysis and flow cytometry.
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| 4. |
- Lisowska, Halina, et al.
(författare)
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The dose-response relationship for dicentric chromosomes and gamma-H2AX foci in human peripheral blood lymphocytes : Influence of temperature during exposure and intra- and inter-individual variability of donors
- 2013
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Ingår i: International Journal of Radiation Biology. - : Informa UK Limited. - 0955-3002 .- 1362-3095. ; 89:3, s. 191-199
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Tidskriftsartikel (refereegranskat)abstract
- Purpose : Hypothermia during in vitro irradiation of human peripheral blood lymphocytes (PBL) affects the level of chromosome aberrations. The molecular mechanisms of this phenomenon are not fully understood. The aim of our study was to examine the effect of hypothermia on the dose-response relationship for dicentric chromosomes and the level of gamma-H2AX (phosphorylated histone H2AX) foci. In addition, the inter- and intra-individual variability was assessed in relation to temperature. Materials and methods : PBL were kept at 0.8, 20 and 37 degrees C and then exposed to gamma-rays (from 0-3 Gy). Dicentric chromosomes were scored in first post-treatment mitoses. gamma H2AX foci were scored 15, 30, 60, 120 min and 24 h post irradiation. Results : Our results revealed that the frequency of dicentric chromosomes in cells exposed at 37 degrees C to gamma-rays was higher than after exposure at 0.8 and 20 degrees C. No effect of temperature was observed on the number of gamma-H2AX foci as well as on the intra-and inter-individual variations of the dicentric yield and the number of gamma-H2AX foci. Conclusions : Temperature at exposure to ionizing radiation has a pronounced effect on the level of cytogenetic damage but not gamma-H2AX foci.
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