| 1. |
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| 2. |
- Karimpour, Masoumeh, et al.
(författare)
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Postprandial metabolomics : A pilot mass spectrometry and NMR study of the human plasma metabolome in response to a challenge meal
- 2016
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Ingår i: Analytica Chimica Acta. - Elsevier : Elsevier BV. - 0003-2670 .- 1873-4324. ; 908, s. 121-131
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Tidskriftsartikel (refereegranskat)abstract
- The study of postprandial metabolism is relevant for understanding metabolic diseases and characterizing personal responses to diet. We combined three analytical platforms – gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) – to validate a multi-platform approach for characterizing individual variation in the postprandial state. We analyzed the postprandial plasma metabolome by introducing, at three occasions, meal challenges on a usual diet, and 1.5 years later, on a modified background diet. The postprandial response was stable over time and largely independent of the background diet as revealed by all three analytical platforms. Coverage of the metabolome between NMR and GC-MS included more polar metabolites detectable only by NMR and more hydrophobic compounds detected by GC-MS. The variability across three separate testing occasions among the identified metabolites was in the range of 1.1–86% for GC-MS and 0.9–42% for NMR in the fasting state at baseline. For the LC-MS analysis, the coefficients of variation of the detected compounds in the fasting state at baseline were in the range of 2–97% for the positive and 4–69% for the negative mode. Multivariate analysis (MVA) of metabolites detected with GC-MS revealed that for both background diets, levels of postprandial amino acids and sugars increased whereas those of fatty acids decreased at 0.5 h after the meal was consumed, reflecting the expected response to the challenge meal. MVA of NMR data revealed increasing postprandial levels of amino acids and other organic acids together with decreasing levels of acetoacetate and 3-hydroxybutanoic acid, also independent of the background diet. Together these data show that the postprandial response to the same challenge meal was stable even though it was tested 1.5 years apart, and that it was largely independent of background diet. This work demonstrates the efficacy of a multi-platform metabolomics approach followed by multivariate and univariate data analysis for a broad-scale screen of the individual metabolome, particularly for studies using repeated measures to determine dietary response phenotype.
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| 3. |
- Pathan, Meerakhan, et al.
(författare)
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Plasma metabolite markers of parkinson’s disease and atypical parkinsonism
- 2021
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Ingår i: Metabolites. - : MDPI. - 2218-1989 .- 2218-1989. ; 11:12
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Tidskriftsartikel (refereegranskat)abstract
- Differentiating between Parkinson’s disease (PD) and the atypical Parkinsonian disorders of multiple system atrophy (MSA) and progressive supranuclear palsy (PSP) is difficult clinically due to overlapping symptomatology, especially at early disease stages. Consequently, there is a need to identify metabolic markers for these diseases and to develop them into viable biomarkers. In the present investigation, solution nuclear magnetic resonance and mass spectrometry metabolomics were used to quantitatively characterize the plasma metabolomes (a total of 167 metabolites) of a cohort of 94 individuals comprising 34 PD, 12 MSA, and 17 PSP patients, as well as 31 control subjects. The distinct and statistically significant differences observed in the metabolite concentrations of the different disease and control groups enabled the identification of potential plasma metabolite markers of each disorder and enabled the differentiation between the disorders. These group-specific differences further implicate disturbances in specific metabolic pathways. The two metabolites, formic acid and succinate, were altered similarly in all three disease groups when compared to the control group, where a reduced level of formic acid suggested an effect on pyruvate metabolism, methane metabolism, and/or the kynurenine pathway, and an increased succinate level suggested an effect on the citric acid cycle and mitochondrial dysfunction.
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| 4. |
- Surowiec, Izabella, et al.
(författare)
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Multi-platform metabolomics assays for human lung lavage fluids in an air pollution exposure study
- 2016
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Ingår i: Analytical and Bioanalytical Chemistry. - : Springer Science and Business Media LLC. - 1618-2642 .- 1618-2650. ; 408:17, s. 4751-4764
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Tidskriftsartikel (refereegranskat)abstract
- Metabolomics protocols are used to comprehensively characterize the metabolite content of biological samples by exploiting cutting-edge analytical platforms, such as gas chromatography (GC) or liquid chromatography (LC) coupled to mass spectrometry (MS) assays, as well as nuclear magnetic resonance (NMR) assays. We have developed novel sample preparation procedures combined with GC-MS, LC-MS, and NMR metabolomics profiling for analyzing bronchial wash (BW) and bronchoalveolar lavage (BAL) fluid from 15 healthy volunteers following exposure to biodiesel exhaust and filtered air. Our aim was to investigate the responsiveness of metabolite profiles in the human lung to air pollution exposure derived from combustion of biofuels, such as rapeseed methyl ester biodiesel, which are increasingly being promoted as alternatives to conventional fossil fuels. Our multi-platform approach enabled us to detect the greatest number of unique metabolites yet reported in BW and BAL fluid (82 in total). All of the metabolomics assays indicated that the metabolite profiles of the BW and BAL fluids differed appreciably, with 46 metabolites showing significantly different levels in the corresponding lung compartments. Furthermore, the GC-MS assay revealed an effect of biodiesel exhaust exposure on the levels of 1-monostearylglycerol, sucrose, inosine, nonanoic acid, and ethanolamine (in BAL) and pentadecanoic acid (in BW), whereas the LC-MS assay indicated a shift in the levels of niacinamide (in BAL). The NMR assay only identified lactic acid (in BW) as being responsive to biodiesel exhaust exposure. Our findings demonstrate that the proposed multi-platform approach is useful for wide metabolomics screening of BW and BAL fluids and can facilitate elucidation of metabolites responsive to biodiesel exhaust exposure.
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| 5. |
- Wu, Junfang, et al.
(författare)
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Gender differences in the bile acid profiles of APP/PS1 transgenic AD mice
- 2020
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Ingår i: Brain Research Bulletin. - : Elsevier. - 0361-9230 .- 1873-2747. ; 161, s. 116-126
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Tidskriftsartikel (refereegranskat)abstract
- Alzheimer’s disease (AD) is a neurodegenerative disease and presents in the accumulation of amyloid and neurofibrillary tangle. The association between modulations of gut symbiotic microbes with neurological disease via bidirectional gut-brain axis has been well documented. Bile acid (BA) pools in the enterohepatic circulation could be valuable for probing complex biochemical interactions between host and their symbiotic microbiota. Herein we investigated the levels of 28 BAs in several compartments in enterohepatic circulation (including jejunal, ileum, cecum, colon and feces, plasma and liver tissue) by employing an APP/PS1 induced transgenic AD mouse model. We found that BA profiles in AD mice were gender specific. We observed decreased levels of taurine-conjugated primary BAs (TUDCA, TCA, T-α-MCA and T-β-MCA) and increased levels of secondary BA (iso-DCA) in plasma and liver extracts for female AD transgenic mice. In contrast, increased levels of TDCA in liver extracts and decreased levels of T-β-MCA in jejunal content were noted in male AD mice. These observations suggested that perturbations of BA profiles in AD mice displayed clear gender variations. Our study highlighted the roles of gut microbiota on neurodegenerative disease, which could be gender specific.
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| 6. |
- Wu, Jingnan, 1994, et al.
(författare)
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New Electron Acceptor with End-Extended Conjugation for High-Performance Polymer Solar Cells
- 2021
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Ingår i: Energy & Fuels. - : American Chemical Society (ACS). - 1520-5029 .- 0887-0624. ; 35:23, s. 19061-19068
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Tidskriftsartikel (refereegranskat)abstract
- To develop high-efficiency polymer solar cells (PSCs), the acceptors in a bulk heterojunction (BHJ) blend are supposed to possess complementary absorption bands in the near-infrared region and a suitable energy level to be well-matched with the donors. In this work, a new small molecular acceptor (SMA) named IDTT8-N based on an indacenodithienothiophene (IDTT) core was designed and synthesized. In comparison to the counterpart molecule IDTN with an indacenodithiophene (IDT) core, IDTT8-N with the extended π-conjugation length of an IDT core not only exhibits a red shift of ca. 35 nm in optical absorption but also has little change on its lowest unoccupied molecular orbital (LUMO) energy level. Therefore, PSCs based on PM6:IDTT8-N exhibit a superior short-circuit current density (Jsc) and high open-circuit voltage (Voc). Moreover, apart from the strong face-on molecular stacking, distinct end-group π-πstacking of IDTT8-N can be observed in the blends, facilitating the charge transport. Therefore, the optimized PM6:IDTT8-N-based devices exhibit dramatically high and balanced electron mobility (μe) and hole mobility (μh), whose magnitudes are over 10-3 cm2 V-1 s-1. Consequently, an extraordinary PCE of 14.1% with a relatively high Jsc of 20.98 mA cm-2 and a Voc of 0.94 V was recorded. To our knowledge, it is the new record among PSCs with a SMA based on 2-(3-oxocyclopentylidene)malononitrile (INCN) as end groups. These results indicate that extending the π-conjugation length of the fused ring core of a SMA is an efficient method to both enhance the absorption and the molecular interaction of the acceptor as well as the photovoltaic performance of PSCs.
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| 7. |
- Wu, Junfang, et al.
(författare)
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NMR analysis of the CSF and plasma metabolome of rigorously matched amyotrophic lateral sclerosis, Parkinson's disease and control subjects
- 2016
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Ingår i: Metabolomics. - : Springer Science and Business Media LLC. - 1573-3882 .- 1573-3890. ; 12:6
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Tidskriftsartikel (refereegranskat)abstract
- Introduction: Amyotrophic lateral sclerosis (ALS) and Parkinson’s disease (PD) are two severe neurodegenerative disorders for which the disease mechanisms are poorly understood and reliable biomarkers are absent.Objectives: To identify metabolite biomarkers for ALS and PD, and to gain insights into which metabolic pathways are involved in disease.Methods: Nuclear magnetic resonance (NMR) metabolomics was utilized to characterize the metabolite profiles of cerebrospinal fluid (CSF) and plasma from individuals in three age, gender, and sampling-date matched groups, comprising 22 ALS, 22 PD and 28 control subjects.Results: Multivariate analysis of NMR data generated robust discriminatory models for separation of ALS from control subjects. ALS patients showed increased concentrations of several metabolites in both CSF and plasma, these are alanine (CSF fold change = 1.22, p = 0.005), creatine (CSF-fc = 1.17, p = 0.001), glucose (CSF-fc = 1.11, p = 0.036), isoleucine (CSF-fc = 1.24, p = 0.002), and valine (CSF-fc = 1.17, p = 0.014). Additional metabolites in CSF (creatinine, dimethylamine and lactic acid) and plasma (acetic acid, glutamic acid, histidine, leucine, pyruvate and tyrosine) were also important for this discrimination. Similarly, panels of CSF-metabolites that discriminate PD from ALS and control subjects were identified.Conclusions: The results for the ALS patients suggest an affected creatine/creatinine pathway and an altered branched chain amino acid (BCAA) metabolism, and suggest links to glucose and energy metabolism. Putative metabolic markers specific for ALS (e.g. creatinine and lactic acid) and PD (e.g. 3-hydroxyisovaleric acid and mannose) were identified, while several (e.g. creatine and BCAAs) were shared between ALS and PD, suggesting some overlap in metabolic alterations in these disorders.
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| 8. |
- Wu, Junfang, et al.
(författare)
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NMR-based metabolite profiling of human milk : A pilot study of methods for investigating compositional changes during lactation
- 2016
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Ingår i: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier BV. - 0006-291X .- 1090-2104. ; 469:3, s. 626-632
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Tidskriftsartikel (refereegranskat)abstract
- Low-molecular-weight metabolites in human milk are gaining increasing interest in studies of infant nutrition. In the present study, the milk metabolome from a single mother was explored at different stages of lactation. Metabolites were extracted from sample aliquots using either methanol water (MeOH/H2O) extraction or ultrafiltration. Nuclear magnetic resonance (NMR) spectroscopy was used for metabolite identification and quantification, and multi- and univariate statistical data analyses were used to detect changes over time of lactation. Compared to MeOH/H2O extraction, ultrafiltration more efficiently reduced the interference from lipid and protein resonances, thereby enabling the identification and quantification of 36 metabolites. The human milk metabolomes at the early (9-24 days after delivery) and late (31-87 days after delivery) stages of lactation were distinctly different according to multi- and univariate statistics. The late lactation stage was characterized by significantly elevated concentrations of lactose, choline, alanine, glutamate, and glutamine, as well as by reduced levels of citrate, phosphocholine, glycerophosphocholine, and N-acetylglucosamine. Our results indicate that there are significant compositional changes of the human milk metabolome also in different phases of the matured lactation stage. These findings complement temporal studies on the colostrum and transitional metabolome in providing a better understanding of the nutritional variations received by an infant.
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| 9. |
- Yan, Xin, et al.
(författare)
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Highly efficient ternary solar cells with reduced non-radiative energy loss and enhanced stability via two compatible non-fullerene acceptors
- 2022
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Ingår i: Journal of Materials Chemistry A. - : ROYAL SOC CHEMISTRY. - 2050-7488 .- 2050-7496. ; 10:29, s. 15605-15613
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Tidskriftsartikel (refereegranskat)abstract
- A ternary strategy by introducing a third component into a binary host system has been proven to be a simple and promising method to boost the power conversion efficiency (PCE) and stability of organic solar cells (OSCs). Herein, a high efficient ternary OSC is fabricated, wherein, a non-fullerene acceptor, namely MOIT-M, is introduced as a third component into the PM6:BTP-eC9 blend. MOIT-M possesses good complementary absorption spectra and aligned cascade energy levels with the host binary blend, which benefits light harvesting, exciton dissociation, and charge transport. Moreover, MOIT-M exhibits good miscibility with BTP-eC9, forming a well-mixed phase, which improves molecular packing for better charge transport and optimizes ternary blend morphology. Notably, the incorporation of MOIT-M suppresses non-radiative recombination, leading to reduced non-radiative energy losses (Delta E-nr). As a result, the ternary OSC exhibits a significantly increased PCE of 18.5% with a lower Delta E-nr of 0.21 eV in comparison with the control binary PM6:BTP-eC9 device with a PCE of 17.4% and a Delta E-nr of 0.24 eV. In addition, the ternary OSC displays better storage stability compared to the PM6:BTP-eC9 system. This work indicates that a ternary strategy via combining two compatible small molecule acceptors is effective to simultaneously improve the efficiency and stability of OSCs.
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| 10. |
- Zhang, Zhiliang, et al.
(författare)
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Enhancing intermolecular packing and light harvesting through asymmetric non-fullerene acceptors for achieving 18.7% efficiency ternary organic solar cells
- 2023
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Ingår i: Journal of Materials Chemistry A. - : ROYAL SOC CHEMISTRY. - 2050-7488 .- 2050-7496. ; 11:28, s. 15553-15560
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Tidskriftsartikel (refereegranskat)abstract
- In recent years, the ternary strategy has been proven to be an effective way to improve the performance of organic solar cells (OSCs). Herein, an asymmetric medium-band gap non-fullerene acceptor (AFIC) is synthesized and added as the third component into the PM6:BTP-eC9 binary blend for a highly efficient ternary OSC. AFIC exhibits a well-complementary absorption spectrum with the host binary blend, which benefits light harvesting of the active layer. Furthermore, AFIC shows a large dipole moment and good miscibility with BTP-eC9, which facilitates the formation of a stable well-mixed phase and enhances molecular packing in the blend, leading to improved charge transport and suppressed charge recombination in ternary devices. As a result, the ternary OSC based on PM6:BTP-eC9:AFIC demonstrates a significantly improved power conversion efficiency (PCE) of 18.7% while the binary OSC based on PM6:BTP-eC9 shows a PCE of 17.5%, which is attributed to the synergistic enhancement of the open-circuit voltage (V-oc), short-circuit current density (J(sc)), and fill factor (FF).
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