| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Ding, Huaiyi, et al.
(författare)
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Maximizing Integrated Optical and Electrical Properties of a Single ZnO Nanowire through Native Interfacial Doping
- 2014
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Ingår i: Advanced Materials. - : Wiley. - 0935-9648 .- 1521-4095. ; 26:19, s. 3035-3041
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Tidskriftsartikel (refereegranskat)abstract
- A native interfacial doping layer introduced in core-shell type ZnO nanowires by a simple vapor phase re-growth procedure endows the produced nanowires with both excellent electrical and optical performances compared to conventional homogeneous ZnO nanowires. The unique Zn-rich interfacial structure in the core-shell nanowires plays a crucial role in the outstanding performances.
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| 3. |
- Liu, Shengchun, et al.
(författare)
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Comparative analysis of air and CO2 as working fluids for compressed and liquefied gas energy storage technologies
- 2019
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Ingår i: Energy Conversion and Management. - : Elsevier BV. - 0196-8904 .- 1879-2227. ; feb, s. 608-620
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Tidskriftsartikel (refereegranskat)abstract
- With the large-scale use of intermittent renewable energy worldwide, such as wind energy and solar energy, energy storage systems are urgently needed and have been rapidly developed. Technologies of compressed gas energy storage (CGES) and liquefied gas energy storage (LGES) are playing an important role, and air has been commonly used as working fluid. CO2 is another potential working fluid and attracting more and more attention due to the rise of CO2 capture and utilization. However, it is still unclear which is the better working fluid. This paper comparatively analyzed the performance of CGES and LGES systems using air and CO2 as working fluids. Both diabatic and adiabatic CGES are considered. Simulation results show that except diabatic CGES systems, using CO2 could achieve a similar or even higher round-trip efficiency than using air. In addition, the use of CO2 instead of air as a working fluid has additional advantages, such as a lower storage temperature can be achieved at the same storage pressure for the adiabatic CGES system; and a higher condensing temperature can be achieved at the same condensing pressure for the LGES system, which can benefit the system design and operation.
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