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Sökning: WFRF:(Xiao Ziyang)

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1.
  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2.
  • Zhang, Ziyang, et al. (författare)
  • Experimental demonstration of 2D photonic crystal surface cavity in amorphous silicon on silica structure
  • 2007
  • Ingår i: OFC/NFOEC 2007 - Optical Fiber Communication and the National Fiber Optic Engineers Conference 2007. - 9781557528315 - 1557528314 ; , s. 4348573-
  • Konferensbidrag (refereegranskat)abstract
    • Design, fabrication, and characterization of an optical filter based on side coupling between silicon wire waveguide and photonic crystal surface mode cavity in silicon on silica structure is presented for the 1550 nm wavelength spectral region.
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3.
  • Zhang, Ziyang, et al. (författare)
  • Optical filter based on two-dimensional photonic crystal surface-mode cavity in amorphous silicon-on-silica structure
  • 2007
  • Ingår i: Applied Physics Letters. - : AIP Publishing. - 0003-6951 .- 1077-3118. ; 90:4, s. 041108-
  • Tidskriftsartikel (refereegranskat)abstract
    • An optical filter based on side coupling between silicon wire waveguide and two-dimensional photonic crystal surface-mode cavity is presented. The design is optimized numerically by parallel three-dimensional finite-different time-domain simulations. The device is then fabricated on amorphous silicon-on-silica structure. The drop wavelength is observed around 1580 nm. The extinction ratio of the filter is larger than 10 dB and the intrinsic quality factor of the surface-mode cavity is approximately 2000.
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  • Resultat 1-3 av 3

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