| 1. |
- Xie, Bin
(creator_code:cre_t)
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[An optical circulation amplification type biological chip detecting process]
- 2004
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Patent (övrigt vetenskapligt/konstnärligt)abstract
- The invention discloses an optical circulation enlarged type biological chip detecting method, wherein the enzyme recirculation and bioluminescence are combined, for realizing the FMNH#-[2] accumulation through the circulation on the electrodes by the NADH/NAD#+[+] oxidation-reduction, the high level accumulation FMNH#-[2] reacts with other light-emitting bottom luciferase, long chain aldehydes and oxygen, giving off fluorescent light having wavelength of 490nm. The invention realizes high sensibility, low cost and simplicity of operation, thus is suitable for each types of biological sample analysis.
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| 2. |
- Xie, Bin
(creator_code:cre_t)
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氨酰基转运核糖核酸合成酶识别型蛋白芯片的制备方法
- 2004
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Patent (övrigt vetenskapligt/konstnärligt)abstract
- A method of manufacturing a protein chip for identifying aminoacyl-tRNA synthetases, suitable for detecting basic amino acids in proteins. It is characterized in, immoblizing aminoacyl-tRNA synthetases corresponding to 20 basic amino acids in arrays onto the surface of the chip, then fixing 20 kinds of tRNAs labelled by fluorescence onto the corresponding synthetases, then obtaining a chip of aminoacyl-tRNA synthetases. The chip could detect amino acids in proteins. By scanning fluorescence, amino acids in proteins will be determined based on fluorescence of 20 wells.
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| 3. |
- Xie, Weihong, et al.
(författare)
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Pyrroloquinoline quinone glucose dehydrogenase adopted in thermometric analysis for enhancement of glucose determination
- 2018
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Ingår i: Journal of Thermal Analysis and Calorimetry. - : Springer Science and Business Media LLC. - 1388-6150 .- 1588-2926. ; 134:3, s. 1913-1919
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Tidskriftsartikel (refereegranskat)abstract
- A broad measurement range of glucose is often required in clinical analysis, especially for diabetic patients where glucose levels can be very high. Pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) has previously been used in electrochemical quantification of glucose with an extended linear range. However, in real sample determination of glucose, interferences from electroactive substances in blood are unavoidable. Calorimetric biosensors, e.g., the Enzyme Thermistor, are insensitive to either directly electroactive or optical interferences often present in real clinical samples. This paper describes a novel analytical strategy where the intrinsic advantages of PQQGDH are combined with the Enzyme Thermistor as biosensor using calorimetric detection as general measurement principle. When compared with the most frequently used enzyme glucose oxidase, PQQGDH has a higher catalytic efficiency and is insensitive to the availability of oxygen. The use of calorimetry in this context resulted in a broad linear range of glucose measurements, from 0.009 to 100 mM, an excellent specificity and insignificant side effects of compounds present in blood at high concentrations, such as lactate and urea.
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| 4. |
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| 5. |
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| 6. |
- Ding, Yubin, et al.
(författare)
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Acid/Base Switching of the Tautomerism and Conformation of a Dioxoporphyrin for Integrated Binary Subtraction
- 2014
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Ingår i: Chemistry - A European Journal. - : Wiley. - 0947-6539 .- 1521-3765. ; 20:40, s. 12910-12916
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Tidskriftsartikel (refereegranskat)abstract
- Compared with most of the reported logic devices based on the supramolecular approach, systems based on individual molecules can avoid challenging construction requirements. Herein, a novel dioxoporphyrin DPH22 was synthesized and two of its tautomers were characterized by single-crystal X-ray diffraction studies. Compound DPH22 exhibits multichannel controllable stepwise tautomerization, protonation, and deprotonation processes through interactions with H and F- ions. By using the addition of H+ and F- ions as inputs and UVNis absorption values at.1=412, 510, 562, and 603 nm as outputs, the controlled tautomerism of DPH22 has been successfully used for the construction of an integrated molecular level half-subtractor and comparator. In addition, this acid/base-switched tautomerism is reversible, thus endowing the system with ease of reset and recycling; consequently, there is no need to modulate complicated intermolecular interactions and electron-/chargetransfer processes.
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| 7. |
- Ding, Yubin, et al.
(författare)
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alpha-Monoacylated and alpha,alpha '- and alpha,beta '-Diacylated Dipyrrins as Highly Sensitive Fluorescence "Turn-on" Zn2+ Probes
- 2013
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Ingår i: Journal of Organic Chemistry. - : American Chemical Society (ACS). - 0022-3263 .- 1520-6904. ; 78:11, s. 5328-5338
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Tidskriftsartikel (refereegranskat)abstract
- With the purpose of developing readily synthesized CHEF (chelation-enhanced fluorescence) type Zn2+ probes with relatively simple molecular structures and excellent sensing behavior, p-anisoyl chloride was used for the acylation of 5-(pentafluorophenyl)dipyrromethane. Interestingly, the alpha,beta'-diacylated product PS2 with a unique substitution mode was obtained in high yield in addition to the normal a-substituted mono- and diacylated products PSI and PS3. Further oxidation of PS1-PS3 afforded dipyrrins S1-S3. Crystal structure and H-1 NMR measurements of S2 demonstrate the existence of a pure tautomer, which is consistent with DFT calculations. S1-S3 show highly Zn2+ selective "turn-on" fluorescence based on a CHEF mechanism by the formation of 2:1 (probe:metal) Zn2+ complexes. The emission colors can be easily tuned from green to red by changing the dipyrrin substitution modes. Furthermore, these probes demonstrate fast responses and wide applicable pH ranges. Among them, S2 shows the highest Zn2+ sensitivity, with a detection limit of 4.4 x 10(-8) M.
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| 8. |
- Ding, Yubin, et al.
(författare)
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From nonconjugation to conjugation : novel meso-OH substituted dipyrromethanes as fluorescence turn-on Zn2+ probes
- 2013
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Ingår i: Organic and biomolecular chemistry. - : Royal Society of Chemistry (RSC). - 1477-0520 .- 1477-0539. ; 11:16, s. 2685-2692
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Tidskriftsartikel (refereegranskat)abstract
- Most reported Zn2+ probes suffer from the interference of background fluorescence originated from the conjugated structures of commonly utilized fluorophores. In this work, three novel meso-hydroxyl group substituted dipyrromethanes DPMOH1-DPMOH3 were synthesized and found to be colourless and nonfluorescent due to the interruption of the conjugated p system by an sp(3) carbon between the two pyrrolic units. Interestingly, only the addition of Zn2+ to the solutions of DPMOH1-DPMOH3 promoted their oxidation to dipyrrin forms, and bright fluorescence "turn on" was observed due to the formation of corresponding dipyrrin complexes with the dipyrrin : zinc stoichiometry of 2 :1. Zn2+ detection mechanism was investigated by UV-Vis, fluorescence, H-1 NMR and HRMS analyses, which can be ascribed to the CHEF type fluorescence enhancement, resulting from good rigidity of the dipyrrin complexes. Hence, DPMOH1-DPMOH3 can be used as fluorescence turn-on Zn2+ probes with the advantage of no background fluorescence.
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| 9. |
- Li, Shian, et al.
(författare)
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Numerical Predictions of Pressure Drop and Heat Transfer in a Blade Internal Cooling Passage with Continuous/Truncated Ribs
- 2012
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Ingår i: Heat Transfer Research. - 1064-2285. ; 43:6, s. 573-590
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Tidskriftsartikel (refereegranskat)abstract
- Ribs are often used in the mid-section of internal turbine blades to augment heat transfer from the blade wall to a coolant, but most research works are concerned only with continuous ribs attached to the side walls. In this paper, a turbulent flow and heat transfer of a rectangular passage with continuous and truncated ribs on opposite walls have been predicted numerically. Two types of ribs are studied: 90-deg ribs and 45-deg V-shaped ribs. The inlet Reynolds numbers range from 12,000 to 60,000. The complex three-dimensional turbulent flows inside the blade internal coolant passage and heat transfer between the rib-walls and side-walls are presented. The overall performances of six different ribbed passages are evaluated and compared. Numerical results show that the passage with truncated V-shaped ribs is very effective in improving the heat transfer performance with a low pressure drop.
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| 10. |
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