| 1. |
- Beal, Jacob, et al.
(author)
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Robust estimation of bacterial cell count from optical density
- 2020
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In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Journal article (peer-reviewed)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
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- 2019
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Journal article (peer-reviewed)
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| 4. |
- Jin, Ying-Hui, et al.
(author)
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Chemoprophylaxis, diagnosis, treatments, and discharge management of COVID-19 : An evidence-based clinical practice guideline (updated version)
- 2020
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In: Military Medical Research. - : Springer Science and Business Media LLC. - 2054-9369. ; 7:1
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Journal article (peer-reviewed)abstract
- The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of a rapidly spreading illness, coronavirus disease 2019 (COVID-19), affecting more than seventeen million people around the world. Diagnosis and treatment guidelines for clinicians caring for patients are needed. In the early stage, we have issued "A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus (2019-nCoV) infected pneumonia (standard version)"; now there are many direct evidences emerged and may change some of previous recommendations and it is ripe for develop an evidence-based guideline. We formed a working group of clinical experts and methodologists. The steering group members proposed 29 questions that are relevant to the management of COVID-19 covering the following areas: chemoprophylaxis, diagnosis, treatments, and discharge management. We searched the literature for direct evidence on the management of COVID-19, and assessed its certainty generated recommendations using the Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach. Recommendations were either strong or weak, or in the form of ungraded consensus-based statement. Finally, we issued 34 statements. Among them, 6 were strong recommendations for, 14 were weak recommendations for, 3 were weak recommendations against and 11 were ungraded consensus-based statement. They covered topics of chemoprophylaxis (including agents and Traditional Chinese Medicine (TCM) agents), diagnosis (including clinical manifestations, reverse transcription-polymerase chain reaction (RT-PCR), respiratory tract specimens, IgM and IgG antibody tests, chest computed tomography, chest x-ray, and CT features of asymptomatic infections), treatments (including lopinavir-ritonavir, umifenovir, favipiravir, interferon, remdesivir, combination of antiviral drugs, hydroxychloroquine/chloroquine, interleukin-6 inhibitors, interleukin-1 inhibitors, glucocorticoid, qingfei paidu decoction, lianhua qingwen granules/capsules, convalescent plasma, lung transplantation, invasive or noninvasive ventilation, and extracorporeal membrane oxygenation (ECMO)), and discharge management (including discharge criteria and management plan in patients whose RT-PCR retesting shows SARS-CoV-2 positive after discharge). We also created two figures of these recommendations for the implementation purpose. We hope these recommendations can help support healthcare workers caring for COVID-19 patients.
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| 5. |
- Kristanl, Matej, et al.
(author)
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The Seventh Visual Object Tracking VOT2019 Challenge Results
- 2019
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In: 2019 IEEE/CVF INTERNATIONAL CONFERENCE ON COMPUTER VISION WORKSHOPS (ICCVW). - : IEEE COMPUTER SOC. - 9781728150239 ; , s. 2206-2241
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Conference paper (peer-reviewed)abstract
- The Visual Object Tracking challenge VOT2019 is the seventh annual tracker benchmarking activity organized by the VOT initiative. Results of 81 trackers are presented; many are state-of-the-art trackers published at major computer vision conferences or in journals in the recent years. The evaluation included the standard VOT and other popular methodologies for short-term tracking analysis as well as the standard VOT methodology for long-term tracking analysis. The VOT2019 challenge was composed of five challenges focusing on different tracking domains: (i) VOT-ST2019 challenge focused on short-term tracking in RGB, (ii) VOT-RT2019 challenge focused on "real-time" short-term tracking in RGB, (iii) VOT-LT2019 focused on long-term tracking namely coping with target disappearance and reappearance. Two new challenges have been introduced: (iv) VOT-RGBT2019 challenge focused on short-term tracking in RGB and thermal imagery and (v) VOT-RGBD2019 challenge focused on long-term tracking in RGB and depth imagery. The VOT-ST2019, VOT-RT2019 and VOT-LT2019 datasets were refreshed while new datasets were introduced for VOT-RGBT2019 and VOT-RGBD2019. The VOT toolkit has been updated to support both standard short-term, long-term tracking and tracking with multi-channel imagery. Performance of the tested trackers typically by far exceeds standard baselines. The source code for most of the trackers is publicly available from the VOT page. The dataset, the evaluation kit and the results are publicly available at the challenge website(1).
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| 6. |
- Yan, Weirong, et al.
(author)
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ISS - An Electronic Syndromic Surveillance System for Infectious Disease in Rural China
- 2013
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In: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 8:4, s. e62749-
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Journal article (peer-reviewed)abstract
- Background: syndromic surveillance system has great advantages in promoting the early detection of epidemics and reducing the necessities of disease confirmation, and it is especially effective for surveillance in resource poor settings. However, most current syndromic surveillance systems are established in developed countries, and there are very few reports on the development of an electronic syndromic surveillance system in resource-constrained settings. Objective: this study describes the design and pilot implementation of an electronic surveillance system (ISS) for the early detection of infectious disease epidemics in rural China, complementing the conventional case report surveillance system. Methods: ISS was developed based on an existing platform 'Crisis Information Sharing Platform' (CRISP), combining with modern communication and GIS technology. ISS has four interconnected functions: 1) work group and communication group; 2) data source and collection; 3) data visualization; and 4) outbreak detection and alerting. Results: As of Jan. 31st 2012, ISS has been installed and pilot tested for six months in four counties in rural China. 95 health facilities, 14 pharmacies and 24 primary schools participated in the pilot study, entering respectively 74256, 79701, and 2330 daily records into the central database. More than 90% of surveillance units at the study sites are able to send daily information into the system. In the paper, we also presented the pilot data from health facilities in the two counties, which showed the ISS system had the potential to identify the change of disease patterns at the community level. Conclusions: The ISS platform may facilitate the early detection of infectious disease epidemic as it provides near real-time syndromic data collection, interactive visualization, and automated aberration detection. However, several constraints and challenges were encountered during the pilot implementation of ISS in rural China.
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| 7. |
- Yang, Tao, et al.
(author)
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A spatial assessment of hydrologic alteration caused by dam construction in the middle and lower Yellow River, China
- 2008
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In: Hydrological Processes. - : Wiley. - 0885-6087 .- 1099-1085. ; 22:18, s. 3829-3843
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Journal article (peer-reviewed)abstract
- The ‘range of variability approach’ (RVA) and mapping technique are used to investigate the spatial variability of hydrologic alterations (HA) due to dam construction along the middle and lower Yellow River, China, over the past live decades. The impacts of climate variability oil hydrological process have been removed during wet and dry periods and the focus IS Oil the impacts of human activities, Such as dam construction, on hydrological processes. Results indicate the following: (1) The impacts of the Sanmenxia reservoir oil the hydrologic alteration are relatively slight with a mean HA value of 0.48, ranking in the last place among the four large reservoirs. (2) Xiaolangdi reservoir has significantly changed the natural flow regime downstream with mean HA value of 0.56, ranking it in first place among the large reservoirs. (3) The results of ranked median degrees of 33 hydrologic alteration indicators for 10 stations in the Yellow River show that the hydrologic alteration of Huayuankou ranks the highest among 10 stream gauges. (4) Impacts of reservoirs on hydrological processes downstream of the dams are closely associated with the regulating activities of the reservoirs. At the same time, alterations of streamflow regimes resulting from climatic changes (e.g. precipitation variability) make the situation more complicated and more hydrological observations will be necessary for further analysis. The results of the current study will be greatly beneficial to the regional water resources management and restoration of eco-environmental systems in the middle and lower Yellow River characterized by intensified dam construction under a changing environment. Copyright (C) 2008 John Wiley & Sons, Ltd.
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| 8. |
- Yang, Tao, et al.
(author)
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Assessing the impact of human activities on hydrological and sediment changes (1953–2000) in nine major catchments of the Loess Plateau, China
- 2010
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In: Rivers Research and Applications. - : Wiley. - 1535-1459 .- 1535-1467. ; 26:3, s. 322-340
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Journal article (peer-reviewed)abstract
- The Range of Variability Approach (RVA) is employed to investigate the variability and spatial patterns of hydrological and sediment changes (1953-2000) induced by intensified human activities, i.e. the implementation of water and soil conservation measures, in nine major catchments of the Loess Plateau, China. Results indicate that: (1) streamflow and sediment load regimes were greatly changed by the implementation of conservation measures; (2) similar spatial patterns of high hydrological and sediment changes resulting from the intensive implementation of conservation measures are observed in most catchments of the middle Yellow River. However, slightly different behaviours of changes exist due to the unique complexity of hydrological and sediment processes in this region and (3) the impacts of various conservation measures on hydrological and sediment processes are closely associated with the extent and types of these measures. Engineering works have a quite immediate impact on streamflow and sediment regimes. Considerable vegetation controls are recognized as additional important driving forces for high hydrological and sediment alterations among various soil conservation measures. In vegetation controls, afforestation is the major factor causing the changes of runoff and sediment processes in these nine catchments. The results of the current study will be greatly beneficial to the regional water resources management and restoration of eco-environmental system in the middle Yellow River basin characterized by intensified soil-conservation measures under the changing environment. Copyright (C) 2009 John Wiley & Sons, Ltd.
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| 9. |
- Zhang, Qi, et al.
(author)
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Muscle-like Artificial Molecular Actuators for Nanoparticles
- 2018
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In: Chem. - : Cell Press. - 2451-9308 .- 2451-9294. ; 4:11, s. 2670-2684
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Journal article (peer-reviewed)abstract
- Muscle tissue performs crucial contraction/extension motions that generate mechanical force and work by consuming chemical energy. Inspired by this naturally created biomolecular machine, artificial molecular muscles are designed and synthesized to undertake linear actuation functions. However, most of these muscle-like actuators are performed at large ensembles, while to realize the nanoscale actuation at the single-to few-molecule level remains challenging. Herein, we developed an artificial muscle-like molecular actuator that can reversibly control the proximity of the attached nano-objects, gold nanoparticles, within the single-molecule length level by its stimuli-responsive muscle-like linear contraction/extension motion. The molecular actuation motion is accompanied by an optical signal output resulting from the plasmonic resonance properties of gold nanoparticles. Meanwhile, the thermal noise of the muscle-like molecular actuator can be overcome by integrating the optical signal over a sufficiently long period.
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| 10. |
- Zhang, Xinhai, et al.
(author)
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Finding critical scenarios for automated driving systems : The data extraction form
- 2021
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Reports (other academic/artistic)abstract
- This is the data extraction form for the systematic literature review work for finding critical scenarios for automated driving systems. The extracted data from the primary studies is structured in the following tables. Primary studies in Tables 1 to 5 correspond to the five clusters defined in Section 6 of the main paper. Please note that some primary studies in these tables are classified as out of the scope of the literature study. These studies are marked in the Purpose column. Primary studies in Tables 6 and 7 are eventually considered as out of the scope. The tables are designed aligned with the taxonomy proposed in Section 4 of the main paper.
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