| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Liu, Jianan, et al.
(författare)
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Deep Instance Segmentation with Automotive Radar Detection Points
- 2023
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Ingår i: IEEE Transactions on Intelligent Vehicles. - 2379-8858. ; 8:1, s. 84-94
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Tidskriftsartikel (refereegranskat)abstract
- Automotive radar provides reliable environmental perception in all-weather conditions with affordable cost, but it hardly supplies semantic and geometry information due to the sparsity of radar detection points. With the development of automotive radar technologies in recent years, instance segmentation becomes possible by using automotive radar. Its data contain contexts such as radar cross section and micro-Doppler effects, and sometimes can provide detection when the field of view is obscured. The outcome from instance segmentation could be potentially used as the input of trackers for tracking targets. The existing methods often utilize a clustering-based classification framework, which fits the need of real-time processing but has limited performance due to minimum information provided by sparse radar detection points. In this paper, we propose an efficient method based on clustering of estimated semantic information to achieve instance segmentation for the sparse radar detection points. In addition, we show that the performance of the proposed approach can be further enhanced by incorporating the visual multi-layer perceptron. The effectiveness of the proposed method is verified by experimental results on the popular RadarScenes dataset, achieving 89.53% mean coverage and 86.97% mean average precision with the IoU threshold of 0.5, which is superior to other approaches in the literature. More significantly, the consumed memory is around 1MB, and the inference time is less than 40ms, indicating that our proposed algorithm is storage and time efficient. These two criteria ensure the practicality of the proposed method in real-world systems.
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| 3. |
- Xiong, Weiyi, et al.
(författare)
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Contrastive Learning for Automotive mmWave Radar Detection Points Based Instance Segmentation
- 2022
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Ingår i: IEEE Conference on Intelligent Transportation Systems, Proceedings, ITSC. ; 2022-October, s. 1255-1261
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Konferensbidrag (refereegranskat)abstract
- The automotive mmWave radar plays a key role in advanced driver assistance systems (ADAS) and autonomous driving. Deep learning-based instance segmentation enables real-time object identification from the radar detection points. In the conventional training process, accurate annotation is the key. However, high-quality annotations of radar detection points are challenging to achieve due to their ambiguity and sparsity. To address this issue, we propose a contrastive learning approach for implementing radar detection points-based instance segmentation. We define the positive and negative samples according to the ground-truth label, apply the contrastive loss to train the model first, and then perform fine-tuning for the following downstream task. In addition, these two steps can be merged into one, and pseudo labels can be generated for the unlabeled data to improve the performance further. Thus, there are four different training settings for our method. Experiments show that when the ground-truth information is only available for a small proportion of the training data, our method still achieves a comparable performance to the approach trained in a supervised manner with 100% ground-truth information.
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| 4. |
- Xiong, Weiyi, et al.
(författare)
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LXL: LiDAR Excluded Lean 3D Object Detection with 4D Imaging Radar and Camera Fusion
- 2024
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Ingår i: IEEE Transactions on Intelligent Vehicles. - 2379-8858. ; 9:1, s. 79-92
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Tidskriftsartikel (refereegranskat)abstract
- As an emerging technology and a relatively affordable device, the 4D imaging radar has already been confirmed effective in performing 3D object detection in autonomous driving. Nevertheless, the sparsity and noisiness of 4D radar point clouds hinder further performance improvement, and in-depth studies about its fusion with other modalities are lacking. On the other hand, as a new image view transformation strategy, “sampling” has been applied in a few image-based detectors and shown to outperform the widely applied “depth-based splatting” proposed in Lift-Splat-Shoot (LSS), even without image depth prediction. However, the potential of “sampling” is not fully unleashed. This paper investigates the “sampling” view transformation strategy on the camera and 4D imaging radar fusion-based 3D object detection. LiDAR Excluded Lean (LXL) model, predicted image depth distribution maps and radar 3D occupancy grids are generated from image perspective view (PV) features and radar bird's eye view (BEV) features, respectively. They are sent to the core of LXL, called “radar occupancy-assisted depth-based sampling”, to aid image view transformation. We demonstrated that more accurate view transformation can be performed by introducing image depths and radar information to enhance the “sampling” strategy. Experiments on VoD and TJ4DRadSet datasets show that the proposed method outperforms the state-of-the-art 3D object detection methods by a significant margin without bells and whistles. Ablation studies demonstrate that our method performs the best among different enhancement settings.
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