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- 2019
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Tidskriftsartikel (refereegranskat)
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| 2. |
- Zhang, Feifei, et al.
(författare)
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Diversity of the Gut Microbiota in Dihydrotestosterone-Induced PCOS Rats and the Pharmacologic Effects of Diane-35, Probiotics, and Berberine. : Gut Microbiota in Dihydrotestosterone-Induced PCOS Rats
- 2019
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Ingår i: Frontiers in microbiology. - : Frontiers Media SA. - 1664-302X. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- Polycystic ovary syndrome (PCOS) is a frequent endocrine and metabolic syndrome in reproductive-age women. Recently, emerging evidence has shown that gut microbiota is closely related to metabolic diseases such as type 2 diabetes, obesity and PCOS. In the present study, we established dihydrotestosterone (DHT)-induced PCOS rats and used Illumina MiSeq sequencing (PE300) to examine the composition, diversity, and abundance of the gut microbiota in PCOS. We compared the effects of three PCOS treatments: Diane-35 (estrogen and progesterone), probiotics and berberine. The DHT-induced rats showed constant estrous cycles, the loss of mature ovarian follicles, insulin resistance and obesity. The reproductive and metabolic functions in the PCOS rats were improved by treatment with Diane-35 and probiotics. Diane-35 and probiotics could restore the diversity of the gut microbiota, and the recovery of gut microbiota disorders improved the reproductive function in PCOS-like rats. However, berberine drastically reduced the species diversity and amount of gut microbiota and showed no improvement in PCOS. The findings of this study will help us to better understand the influence of the gut microbiota in the metabolic and reproductive alterations in PCOS as well as suggest opportunities for future personal dietary guidance for PCOS.
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| 3. |
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| 4. |
- Cao, Qing, et al.
(författare)
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On the predictability of daily rainfall during rainy season over the Huaihe River Basin
- 2019
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Ingår i: Water. - : MDPI AG. - 2073-4441. ; 11:5
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Tidskriftsartikel (refereegranskat)abstract
- In terms of climate change and precipitation, there is large interest in how large-scale climatic features affect regional rainfall amount and rainfall occurrence. Large-scale climate elements need to be downscaled to the regional level for hydrologic applications. Here, a new Nonhomogeneous Hidden Markov Model (NHMM) called the Bayesian-NHMM is presented for downscaling and predicting of multisite daily rainfall during rainy season over the Huaihe River Basin (HRB). The Bayesian-NHMM provides a Bayesian method for parameters estimation. The model avoids the risk to have no solutions for parameter estimation, which often occurs in the traditional NHMM that uses point estimates of parameters. The Bayesian-NHMM accurately captures seasonality and interannual variability of rainfall amount and wet days during the rainy season. The model establishes a link between large-scale meteorological characteristics and local precipitation patterns. It also provides a more stable and efficient method to estimate parameters in the model. These results suggest that prediction of daily precipitation could be improved by the suggested new Bayesian-NHMM method, which can be helpful for water resources management and research on climate change.
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| 5. |
- Cui, Peng, et al.
(författare)
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Long-term androgen excess induces insulin resistance and non-alcoholic fatty liver disease in PCOS-like rats.
- 2021
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Ingår i: The Journal of steroid biochemistry and molecular biology. - : Elsevier BV. - 1879-1220 .- 0960-0760. ; 208
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Tidskriftsartikel (refereegranskat)abstract
- Women with polycystic ovary syndrome (PCOS) are at higher risk for metabolic disorders compared to healthy women, and about 51 % of women with PCOS suffer from non-alcoholic fatty liver disease (NAFLD). Investigation into the pathological mechanism behind this association will provide insights for the prevention and treatment of this complication.Dihydrotestosterone (DHT), a nonaromatic androgen, was used to mimic the pathological conditions of hyperandrogenism and insulin resistance. Hematoxylin and eosin staining, Oil Red O staining, immunofluorescent staining, Western blots, and qRT-PCR were used to verify the hepatic steatosis and inflammation, and the latter two methods were also used for energy and mitochondrion-related assays. ELISA was used to measure the level of reactive oxygen species.Twelve weeks of DHT exposure led to obesity and insulin resistance as well as hepatic steatosis, lipid deposition, and different degrees of inflammation. The expression of molecules involved in respiratory chain and aerobic respiration processes, such as electron transfer complex II, pyruvate dehydrogenase, and succinate dehydrogenase complex subunit A, was inhibited. In addition, molecules associated with apoptosis and autophagy were also abnormally expressed, such as increased Bak mRNA, an increased activated caspase-3 to caspase-3 ratio, and increased Atg12 protein expression. All of these changes are associated with the mitochondria and lead to lipid deposition and inflammation in the liver.Long-term androgen excess contributes to insulin resistance and hepatic steatosis by affecting mitochondrial function and causing an imbalance in apoptosis and autophagy, thus suggesting the pathogenesis of NAFLD in women with PCOS.
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| 6. |
- Jerlström-Hultqvist, Jon, et al.
(författare)
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Genome analysis and comparative genomics of a Giardia intestinalis assemblage E isolate.
- 2010
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Ingår i: BMC Genomics. - : Springer Science and Business Media LLC. - 1471-2164. ; 11, s. 543-
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Tidskriftsartikel (refereegranskat)abstract
- Background Giardia intestinalis is a protozoan parasite that causes diarrhea in a wide range of mammalian species. To further understand the genetic diversity between the Giardia intestinalis species, we have performed genome sequencing and analysis of a wild-type Giardia intestinalis sample from the assemblage E group, isolated from a pig. Results We identified 5012 protein coding genes, the majority of which are conserved compared to the previously sequenced genomes of the WB and GS strains in terms of microsynteny and sequence identity. Despite this, there is an unexpectedly large number of chromosomal rearrangements and several smaller structural changes that are present in all chromosomes. Novel members of the VSP, NEK Kinase and HCMP gene families were identified, which may reveal possible mechanisms for host specificity and new avenues for antigenic variation. We used comparative genomics of the three diverse Giardia intestinalis isolates P15, GS and WB to define a core proteome for this species complex and to identify lineage-specific genes. Extensive analyses of polymorphisms in the core proteome of Giardia revealed differential rates of divergence among cellular processes. Conclusions Our results indicate that despite a well conserved core of genes there is significant genome variation between Giardia isolates, both in terms of gene content, gene polymorphisms, structural chromosomal variations and surface molecule repertoires. This study improves the annotation of the Giardia genomes and enables the identification of functionally important variation.
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| 7. |
- Jerlström-Hultqvist, Jon, et al.
(författare)
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Hydrogenosomes in the diplomonad Spironucleus salmonicida
- 2013
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Ingår i: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 4, s. 2493-
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Tidskriftsartikel (refereegranskat)abstract
- Acquisition of the mitochondrion is a key event in the evolution of the eukaryotic cell, but diversification of the organelle has occurred during eukaryotic evolution. One example of such mitochondria-related organelles (MROs) are hydrogenosomes, which produce ATP by substrate- level phosphorylation with hydrogen as a byproduct. The diplomonad parasite Giardia intestinalis harbours mitosomes, another type of MRO. Here we identify MROs in the salmon parasite Spironucleus salmonicida with similar protein import and Fe-S cluster assembly machineries as in Giardia mitosomes. We find that hydrogen production is prevalent in the diplomonad genus Spironucleus, and that S. salmonicida MROs contain enzymes characteristic of hydrogenosomes. Evolutionary analyses of known hydrogenosomal components indicate their presence in the diplomonad ancestor, and subsequent loss in Giardia. Our results suggest that hydrogenosomes are metabolic adaptations predating the split between parabasalids and diplomonads, which is deeper than the split between animals and fungi in the eukaryotic tree.
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| 8. |
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| 9. |
- Jiang, Tao, et al.
(författare)
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Guest Editorial : Special issue on flexible and resilient urban energy systems
- 2023
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Ingår i: International Journal of Electrical Power & Energy Systems. - : Elsevier BV. - 0142-0615 .- 1879-3517. ; 154, s. 109439-
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Forskningsöversikt (refereegranskat)abstract
- This guest editorial summarizes the topics and the papers selected for the Special Issue on Flexible and Resilient Urban Energy Systems. After rigorous reviewing process, 21 papers are accepted for publication. These 21 accepted papers cover various aspects of urban energy systems and are distributed as following: situational awareness of urban energy systems (2 papers), quantification metrics of flexibility and resilience of urban energy systems (3 papers), vulnerability modeling of urban energy systems under various extreme events (3 papers), planning of flexible and resilient urban energy systems (4 papers), robust and resilient operation and control of urban energy systems (4 papers), recovery and restoration strategy of urban energy systems (2 papers), and coordination and interoperability of interconnected energy systems (3 papers). The Guest Editorial Board hopes this Special Issue can provide a valuable information for future research and advancements in the field of flexible and resilient urban energy systems.
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| 10. |
- Jimenez-Gonzalez, Alejandro, et al.
(författare)
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Lateral Acquisitions Repeatedly Remodel the Oxygen Detoxification Pathway in Diplomonads and Relatives
- 2019
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Ingår i: Genome Biology and Evolution. - : OXFORD UNIV PRESS. - 1759-6653. ; 11:9, s. 2542-2556
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Tidskriftsartikel (refereegranskat)abstract
- Oxygen and reactive oxygen species (ROS) are important stress factors for cells because they can oxidize many large molecules. Fornicata, a group of flagellated protists that includes diplomonads, have anaerobic metabolism but are still able to tolerate fluctuating levels of oxygen. We identified 25 protein families putatively involved in detoxification of oxygen and ROS in this group using a bioinformatics approach and propose how these interact in an oxygen detoxification pathway. These protein families were divided into a central oxygen detoxification pathway and accessory pathways for the synthesis of nonprotein thiols. We then used a phylogenetic approach to investigate the evolutionary origin of the components of this putative pathway in Diplomonadida and other Fornicata species. Our analyses suggested that the diplomonad ancestor was adapted to low-oxygen levels, was able to reduce O-2 to H2O in a manner similar to extant diplomonads, and was able to synthesize glutathione and L-cysteine. Several genes involved in the pathway have complex evolutionary histories and have apparently been repeatedly acquired through lateral gene transfer and subsequently lost. At least seven genes were acquired independently in different Fornicata lineages, leading to evolutionary convergences. It is likely that acquiring these oxygen detoxification proteins helped anaerobic organisms (like the parasitic Giardia intestinalis) adapt to low-oxygen environments (such as the digestive tract of aerobic hosts).
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