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| 2. |
- Ye, Chao, et al.
(författare)
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Comprehensive understanding of Saccharomyces cerevisiae phenotypes with whole-cell model WM_S288C
- 2020
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Ingår i: Biotechnology and Bioengineering. - : Wiley. - 0006-3592 .- 1097-0290. ; 117:5, s. 1562-1574
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Tidskriftsartikel (refereegranskat)abstract
- Biological network construction for Saccharomyces cerevisiae is a widely used approach for simulating phenotypes and designing cell factories. However, due to a complicated regulatory mechanism governing the translation of genotype to phenotype, precise prediction of phenotypes remains challenging. Here, we present WM_S288C, a computational whole-cell model that includes 15 cellular states and 26 cellular processes and which enables integrated analyses of physiological functions of Saccharomyces cerevisiae. Using WM_S288C to predict phenotypes of S. cerevisiae, the functions of 1140 essential genes were characterized and linked to phenotypes at five levels. During the cell cycle, the dynamic allocation of intracellular molecules could be tracked in real-time to simulate cell activities. Additionally, one-third of non-essential genes were identified to affect cell growth via regulating nucleotide concentrations. These results demonstrated the value of WM_S288C as a tool for understanding and investigating the phenotypes of S. cerevisiae.
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| 3. |
- Chen, Tao, et al.
(författare)
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Update on Novel Non-Operative Treatment for Osteoarthritis : Current Status and Future Trends
- 2021
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Ingår i: Frontiers in Pharmacology. - : Frontiers Media SA. - 1663-9812. ; 12
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Forskningsöversikt (refereegranskat)abstract
- Osteoarthritis (OA) is a leading cause of pain and disability which results in a reduced quality of life. Due to the avascular nature of cartilage, damaged cartilage has a finite capacity for healing or regeneration. To date, conservative management, including physical measures and pharmacological therapy are still the principal choices offered for OA patients. Joint arthroplasties or total replacement surgeries are served as the ultimate therapeutic option to rehabilitate the joint function of patients who withstand severe OA. However, these approaches are mainly to relieve the symptoms of OA, instead of decelerating or reversing the progress of cartilage damage. Disease-modifying osteoarthritis drugs (DMOADs) aiming to modify key structures within the OA joints are in development. Tissue engineering is a promising strategy for repairing cartilage, in which cells, genes, and biomaterials are encompassed. Here, we review the current status of preclinical investigations and clinical translations of tissue engineering in the non-operative treatment of OA. Furthermore, this review provides our perspective on the challenges and future directions of tissue engineering in cartilage regeneration.
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| 4. |
- Dong, Jingran, et al.
(författare)
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Kinetics and mechanism of oxidation of the anti-tubercular prodrug isoniazid and its analog by iridium(IV) as models for biological redox systems
- 2017
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Ingår i: Dalton Transactions. - : Royal Society of Chemistry (RSC). - 1477-9234 .- 1477-9226. ; 46:26, s. 8377-8386
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Tidskriftsartikel (refereegranskat)abstract
- A complex reaction mechanism of oxidation of the anti-tubercular prodrug isoniazid (isonicotinic hydrazide, INH) by [IrCl6]2− as a model for redox processes of such drugs in biological systems has been studied in aqueous solution as a function of pH between 0 and 8.5. Similar experiments have been performed with its isomer nicotinic hydrazide (NH). All reactions are overall second-order, first-order in [IrCl6]2− and hydrazide, and the observed second-order rate constants k′ have been determined as a function of pH. Spectrophotometric titrations indicate a stoichiometry of [Ir(IV)]:[hydrazide] = 4:1. HPLC analysis shows that the oxidation product of INH is isonicotinic acid. The derived reaction mechanism, based on rate law, time-resolved spectra and stoichiometry, involves parallel attacks by [IrCl6]2− on all four protolytic species of INH and NH as rate-determining steps, depending on pH. These steps are proposed to generate two types of hydrazyl free radicals. These radicals react further in three rapid consecutive processes, leading to the final oxidation products. Rate constants for the rate-determining steps have been determined for all protolytic species I–IV of INH and NH. They are used to calculate reactivity–pH diagrams. These diagrams demonstrate that for both systems, species IV is ca. 105 times more reactive in the redox process than the predominant species III at the physiological pH of 7.4. Thus, species IV will be the main reactant, in spite of the fact that its concentration at this pH is extremely low, a fact that has not been considered in previous work. The results indicate that pH changes might be an important factor in the activation process of INH in biological systems also, and that in such systems this process most likely is more complicated than previously assumed
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| 5. |
- Dong, Jingran, et al.
(författare)
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Reactivity of the glutathione species towards the reduction of ormaplatin (or tetraplatin)
- 2016
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Ingår i: Bioorganic and Medicinal Chemistry Letters. - : Elsevier BV. - 0960-894X. ; 26:17, s. 4261-4266
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Tidskriftsartikel (refereegranskat)abstract
- The reduction of ormaplatin (tetraplatin), a prototype for Pt(IV) anticancer prodrugs, by glutathione (GSH) was kinetically characterized over a wide pH range at 25.0 °C and 1.0 M ionic strength. The reduction follows overall second-order kinetics, giving rise to the oxidized glutathione as the oxidation product, which was identified by high-resolution mass spectrometry. The reaction mechanism put forward involves parallel attacks by all the GSH species on the Pt(IV) prodrug as rate-determining steps. All rate constants for the rate-determining steps have been derived for the first time, enabling the construction of the reactivity of GSH species versus their pH distribution diagram. The diagram clearly displays that only one out of the five GSH species is the mainly responsible for the reduction of ormaplatin at the physiological pH of 7.4.
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| 6. |
- Ran, Huashen, et al.
(författare)
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Processing, microstructure and properties of hierarchically porous Cu
- 2016
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Ingår i: Materials Express. - : American Scientific Publishers. - 2158-5849 .- 2158-5857. ; 6:3, s. 271-276
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Tidskriftsartikel (refereegranskat)abstract
- Hierarchically porous Cu with controlled pore characteristics and mechanical properties were fabricated from CuO powders and NaCl as template. NaCl particles were removed by dissolution from the pre-sintered CuO pellet using deionized water. CuO was decomposed to Cu during sintering at 900 °C in vacuum and resulted in porous Cu with bimodal pores. The porosity of porous Cu increased with increased NaCl content in the initial CuO pellet. The compression strength tests suggested that highly porous Cu were suitable for high impact energy absorption applications. Moreover, porosity, pore size and shape and mechanical properties of porous Cu could be tailored by tailoring the morphology and volume fraction of NaCl particles in the initial CuO pellet
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| 7. |
- Tan, Jun, et al.
(författare)
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The Effectiveness of Metagenomic Next-Generation Sequencing in the Diagnosis of Prosthetic Joint Infection : A Systematic Review and Meta-Analysis
- 2022
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Ingår i: Frontiers in cellular and infection microbiology. - : Frontiers Media SA. - 2235-2988. ; 12
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Forskningsöversikt (refereegranskat)abstract
- Background: A prosthetic joint infection (PJI) is a devastating complication following total joint arthroplasties with poor prognosis. Identifying an accurate and prompt diagnostic method is particularly important for PJI. Recently, the diagnostic value of metagenomic next-generation sequencing (mNGS) in detecting PJI has attracted much attention, while the evidence of its accuracy is quite limited. Thus, this study aimed to evaluate the accuracy of mNGS for the diagnosis of PJI. Methods: We summarized published studies to identify the potential diagnostic value of mNGS for PJI patients by searching online databases using keywords such as “prosthetic joint infection”, “PJI”, and “metagenomic sequencing”. Ten of 380 studies with 955 patients in total were included. The included studies provided sufficient data for the completion of 2-by-2 tables. We calculated the sensitivity, specificity, and area under the SROC curve (AUC) to evaluate mNGS for PJI diagnosis. Results: We found that the pooled diagnostic sensitivity and specificity of mNGS for PJI were 0.93 (95% CI, 0.83 to 0.97) and 0.95 (95% CI, 0.92 to 0.97), respectively. Positive and negative likelihood ratios were 18.3 (95% CI, 10.9 to 30.6) and 0.07 (95% CI, 0.03 to 0.18), respectively. The area under the curve was 0.96 (95% CI, 0.93 to 0.97). Conclusion: Metagenomic next-generation sequencing displays high accuracy in the diagnosis of PJI, especially for culture-negative cases.
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| 8. |
- Xu, Haoyuan, et al.
(författare)
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Effect of rare earth doping on electronic and gas-sensing properties of SnO2 nanostructures
- 2022
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Ingår i: Journal of Alloys and Compounds. - : Elsevier. - 0925-8388 .- 1873-4669. ; 909
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Tidskriftsartikel (refereegranskat)abstract
- Tin dioxide (SnO2) and rare earth (Y, La, Pr, Tb, and Er)-doped SnO2 materials were synthesized by a solvothermal method and used for gas sensors. The effect of rare-earth (RE) doping on structural, electronic, and gas-sensing properties of SnO2 has been investigated. According to a comparative study on the gassensing properties of SnO2 and RE-doped SnO2 gas sensors to various testing gases, the RE-doped SnO2 sensors showed enhanced sensitivities to different testing gases. Especially, the Pr-doped SnO2 sensor exhibited outstanding sensing properties to SO2, including a high response of 19.5-50 ppm SO2, excellent selectivity, repaid response and recovery rates, and superior long-term stability. According to the structural analyses, DFT calculation, and the electrochemical measurement of the SnO2 and Pr-SnO2 materials, the improved electron excitation efficiency endowed the Pr-SnO2 with a high density of free electrons that can be trapped by atmospheric oxygen species and participated in SO2-sensing reactions. Moreover, after the Pr doping of SnO2, the enhanced charge carrier transport properties, including prolonged electron lifetime, improved electron diffusion coefficient, and increased effective diffusion length, were conducive to improving the SO2-sensing property.
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| 9. |
- Xu, Haoyuan, et al.
(författare)
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Highly Efficient SO2 Sensing by Light-Assisted Ag/PANI/SnO2 at Room Temperature and the Sensing Mechanism
- 2021
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Ingår i: ACS Applied Materials and Interfaces. - : American Chemical Society (ACS). - 1944-8244 .- 1944-8252. ; 13:41, s. 49194-49205
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Tidskriftsartikel (refereegranskat)abstract
- Sulfur dioxide (SO2) is one of the most hazardous and common environmental pollutants. However, the development of room-temperature SO2 sensors is seriously lagging behind that of other toxic gas sensors due to their poor recovery properties. In this study, a light-assisted SO2 gas sensor based on polyaniline (PANI) and Ag nanoparticle-comodified tin dioxide nanostructures (Ag/PANI/SnO2) was developed and exhibited remarkable SO2 sensitivity and excellent recovery properties. The response of the Ag/PANI/SnO2 sensor (20.1) to 50 ppm SO2 under 365 nm ultraviolet (UV) light illumination at 20 °C was almost 10 times higher than that of the pure SnO2 sensor. Significantly, the UV-assisted Ag/PANI/SnO2 sensor had a rapid response time (110 s) and recovery time (100 s) to 50 ppm SO2, but in the absence of light, the sensors exhibited poor recovery performance or were even severely and irreversibly deactivated by SO2. The UV-assisted Ag/PANI/SnO2 sensor also exhibited excellent selectivity, superior reproducibility, and satisfactory long-term stability at room temperature. The increased charge carrier density, improved charge-transfer capability, and the higher active surface of the Ag/PANI/SnO2 sensor were revealed by electrochemical measurements and endowed with high SO2 sensitivity. Moreover, the light-induced formation of hot electrons in a high-energy state in Ag/PANI/SnO2 significantly facilitated the recovery of SO2 by the gas sensor.
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