| 1. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 2. |
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| 3. |
- Koga, Shigehiro, et al.
(författare)
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In vivo subcellular imaging of tumors in mouse models using a fluorophore-conjugated anti-carcinoembryonic antigen antibody in two-photon excitation microscopy
- 2014
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Ingår i: Cancer Science. - : Wiley. - 1347-9032 .- 1349-7006. ; 105:10, s. 1299-1306
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Tidskriftsartikel (refereegranskat)abstract
- Recently, there has been growing interest in applying fluorescence imaging techniques to the study of various disease processes and complex biological phenomena in vivo. To apply these methods to clinical settings, several groups have developed protocols for fluorescence imaging using antibodies against tumor markers conjugated to fluorescent substances. Although these probes have been useful in macroscopic imaging, the specificity and sensitivity of these methods must be improved to enable them to detect micro-lesions in the early phases of cancer, resulting in better treatment outcomes. To establish a sensitive and highly specific imaging method, we used a fluorophore-conjugated anti-carcinoembryonic antigen (CEA) antibody to perform macroscopic and microscopic in vivo imaging of inoculated cancer cells expressing GFP with or without CEA. Macroscopic imaging by fluorescence zoom microscopy revealed that bio-conjugation of Alexa Fluor 594 to the anti-CEA antibody allowed visualization of tumor mass consisting of CEA-expressing human cancer cells, but the background levels were unacceptably high. In contrast, microscopic imaging using a two-photon excitation microscope and the same fluorescent antibody resulted in subcellular-resolution imaging that was more specific and sensitive than conventional imaging using a fluorescence zoom microscope. These results suggest that two-photon excitation microscopy in conjunction with fluorophore-conjugated antibodies could be widely adapted to detection of cancer-specific cell-surface molecules, both in cancer research and in clinical applications.
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| 4. |
- Nguyen, Thanh N, et al.
(författare)
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Global Impact of the COVID-19 Pandemic on Stroke Volumes and Cerebrovascular Events: A 1-Year Follow-up.
- 2023
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Ingår i: Neurology. - 1526-632X. ; 100:4
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Tidskriftsartikel (refereegranskat)abstract
- Declines in stroke admission, IV thrombolysis (IVT), and mechanical thrombectomy volumes were reported during the first wave of the COVID-19 pandemic. There is a paucity of data on the longer-term effect of the pandemic on stroke volumes over the course of a year and through the second wave of the pandemic. We sought to measure the effect of the COVID-19 pandemic on the volumes of stroke admissions, intracranial hemorrhage (ICH), IVT, and mechanical thrombectomy over a 1-year period at the onset of the pandemic (March 1, 2020, to February 28, 2021) compared with the immediately preceding year (March 1, 2019, to February 29, 2020).We conducted a longitudinal retrospective study across 6 continents, 56 countries, and 275 stroke centers. We collected volume data for COVID-19 admissions and 4 stroke metrics: ischemic stroke admissions, ICH admissions, IVT treatments, and mechanical thrombectomy procedures. Diagnoses were identified by their ICD-10 codes or classifications in stroke databases.There were 148,895 stroke admissions in the 1 year immediately before compared with 138,453 admissions during the 1-year pandemic, representing a 7% decline (95% CI [95% CI 7.1-6.9]; p < 0.0001). ICH volumes declined from 29,585 to 28,156 (4.8% [5.1-4.6]; p < 0.0001) and IVT volume from 24,584 to 23,077 (6.1% [6.4-5.8]; p < 0.0001). Larger declines were observed at high-volume compared with low-volume centers (all p < 0.0001). There was no significant change in mechanical thrombectomy volumes (0.7% [0.6-0.9]; p = 0.49). Stroke was diagnosed in 1.3% [1.31-1.38] of 406,792 COVID-19 hospitalizations. SARS-CoV-2 infection was present in 2.9% ([2.82-2.97], 5,656/195,539) of all stroke hospitalizations.There was a global decline and shift to lower-volume centers of stroke admission volumes, ICH volumes, and IVT volumes during the 1st year of the COVID-19 pandemic compared with the prior year. Mechanical thrombectomy volumes were preserved. These results suggest preservation in the stroke care of higher severity of disease through the first pandemic year.This study is registered under NCT04934020.
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| 5. |
- Aranda Muñoz, Alvaro, et al.
(författare)
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Co-Designing with AI in Sight
- 2022
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Ingår i: Proceedings of the Design Society. - : Cambridge University Press (CUP). - 2732-527X. ; 2, s. 101-110, s. 101-110
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Tidskriftsartikel (refereegranskat)abstract
- Artificial Intelligence offers a wide variety of capabilities that can potentially address people's needs and desires in their specific contexts. This pilot study presents a collaborative method using a deck of AI cards tested with 58 production, AI, and information science students, and experts from an accessible media agency. The results suggest that, with the support of the method and AI cards, participants can ideate and reach conceptual AI solutions. Such conceptualisations can contribute to a more inclusive integration of AI solutions in society.
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| 6. |
- Aranda Muñoz, Alvaro, et al.
(författare)
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THE KARAKURI CARD DECK: CO-DESIGNING INDUSTRIAL IOT CONCEPTUAL SOLUTIONS
- 2020
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Ingår i: Proceedings of INTERNATIONAL DESIGN CONFERENCE – DESIGN 2020. - : Cambridge University Press (CUP). - 2633-7762. ; 1, s. 807-816
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Konferensbidrag (refereegranskat)abstract
- Novel IoT market solutions and research promise IoT modules that do not require programming or electrical setup, yet shop floor personnel need to face problem solving activities to create technical solutions. This paper introduces the Karakuri card deck and presents a case study composed of four workshop sessions in four manufacturing settings, where shop floor personnel tested the cards as a means of ideating and presenting conceptual IoT solutions in the form of diagrams. The results indicate the validity of the proposed conceptual solutions and suggest prototyping as a next step.
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| 7. |
- Aranda Muñoz, Alvaro, et al.
(författare)
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The Karakuri IoT toolkit : a collaborative solution for ideating and prototyping IoT opportunities
- 2024
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Ingår i: Proceedings of the Design Society. - 2732-527X. ; 4, s. 185-194
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Tidskriftsartikel (refereegranskat)abstract
- This paper presents a collaborative solution developed to enable people without prior Internet of Things (IoT) knowledge to ideate, conceptualise, role-play and prototype potential improvements to their work processes and environments. The solution, called the Karakuri IoT toolkit and method, was tested in two workshops with eight production leaders at a Swedish manufacturing company. Outcomes were analysed from the perspectives of materials interaction and instruments of inquiry. Results indicate the solution can help people conceive and prototype improvement ideas at early design stages.
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| 8. |
- Aranda Muñoz, Alvaro, et al.
(författare)
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The Karakuri IoT toolkit : a collaborative solution for ideating and prototyping IoT opportunities
- 2024
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Ingår i: <em>Proceedings of the Design Society</em>. - : Cambridge University Press. ; 4, s. 185-194
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Konferensbidrag (refereegranskat)abstract
- This paper presents a collaborative solution developed to enable people without prior Internet of Things (IoT) knowledge to ideate, conceptualise, role-play and prototype potential improvements to their work processes and environments. The solution, called the Karakuri IoT toolkit and method, was tested in two workshops with eight production leaders at a Swedish manufacturing company. Outcomes were analysed from the perspectives of materials interaction and instruments of inquiry. Results indicate the solution can help people conceive and prototype improvement ideas at early design stages.
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| 9. |
- Aranda Muñoz, Alvaro, et al.
(författare)
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TO SUPPORT IOT COLLABORATIVE EXPRESSIVENESS ON THE SHOP FLOOR
- 2021
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Ingår i: Proceedings of the Design Society. - : Cambridge University Press (CUP). - 2732-527X. ; 1, s. 3149-3158
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Tidskriftsartikel (refereegranskat)abstract
- The availability of new research for IoT support and the human-centric perspective of industry 4.0 opens a gap to support operators in unleashing their creativity so they can provide improvements opportunities with IoT technology. This paper presents a case-study carried out in four Swedish manufacturing companies, where four different workshops were facilitated to support operators in the conceptualization of manufacturing improvements with IoT technologies. The empirical material gathered during these workshops has been analyzed in five different reflective sessions and discussed in light of previous research from industry 4.0, operators, and IoT support. Results indicate that operators can collaboratively create conceptual IoT solutions and that expressiveness in communicating their ideas and needs using IoT technology is more relevant than technical aspects and details of their proposed IoT solutions. This technological expressiveness is identified as a necessary skill to be cultivated on the shop floor and can potentially contribute to making a more effective and socially sustainable industrial landscape in the future.
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