| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Liu, Qi, et al.
(författare)
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Surface passivation and hole extraction : Bifunctional interfacial engineering toward high-performance all-inorganic CsPbIBr2 perovskite solar cells with efficiency exceeding 12%
- 2022
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Ingår i: Journal of Energy Challenges and Mechanics. - : Elsevier BV. - 2056-9386. ; 74, s. 387-393
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Tidskriftsartikel (refereegranskat)abstract
- All-inorganic CsPbIBr2 perovskite solar cells (PSCs) have attracted considerable research attention in recent years due to their excellent thermal stability. However, their power conversion efficiencies (PCEs) are relatively low and still far below the theoretical limit. Here, we report the use of an organic dye molecule (namely VG1-C8) as a bifunctional interlayer between perovskite and the hole-transport layer in CsPbIBr2 PSCs. Combined experimental and theoretical calculation results disclose that the mul-tiple Lewis base sites in VG1-C8 can effectively passivate the trap states on the perovskite films. Meanwhile, the p-conjugated dye molecule significantly accelerates the hole extraction from the per-ovskite absorber as evidenced by the photoluminescence analysis. Consequently, the VG1-C8 treatment simultaneously boosts the photovoltage and photocurrent density values from 1.26 V and 10.80 mA cm -2 to 1.31 V and 12.44 mA cm -2, respectively. This leads to a significant enhancement of PCE from 9.20% to 12.10% under one sun irradiation (AM 1.5G). To our knowledge, this is the record efficiency reported so far for CsPbIBr2 PSCs. Thus, the present work demonstrates an effective interfacial passivation strategy for the development of highly efficient PSCs.
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| 3. |
- Wei, Yun, et al.
(författare)
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Inverse design of aircraft cabin ventilation by integrating three methods
- 2019
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Ingår i: Building and Environment. - : PERGAMON-ELSEVIER SCIENCE LTD. - 0360-1323 .- 1873-684X. ; 150, s. 33-43
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Tidskriftsartikel (refereegranskat)abstract
- To create a healthy and comfortable aircraft cabin, air-supply parameters of the cabin ventilation system must be designed appropriately. Several methods, such as the computational fluid dynamics (CFD)-based genetic algorithm, CFD-based adjoint method and CFD-based proper orthogonal decomposition (POD), have been developed in recent years for conducing an inverse design. The target environmental performance is specified first, and then the corresponding air-supply parameters are inversely solved with the use of a particular method. However, each method has its pros and cons in terms of efficiency and accuracy. To expedite the inverse design process, this study proposed to integrate the above three methods. The genetic algorithm was adopted first to circumscribe ranges of the air-supply parameters. Next, POD was applied to further narrow the ranges and estimate the optimal air-supply parameters for each design criterion. Finally, the estimated optimal design from POD was supplied to the adjoint method for fine tuning. The above strategy was applied to a five-row aircraft cabin to determine the air-supply opening sizes, directions and temperatures. Criteria that had been proposed specifically for aircraft cabins were used as design targets. Results show that the proposed integration was able to provide the optimal design for each design target. The integrated optimal design was superior to the design provided by each individual method. The bottleneck in further acceleration of the integrated design was the hundreds of design cases resolved by full CFD simulation.
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| 4. |
- Yao, Lei, et al.
(författare)
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Atmospheric new particle formation from sulfuric acid and amines in a Chinese megacity
- 2018
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 361:6399, s. 278-281
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Tidskriftsartikel (refereegranskat)abstract
- Atmospheric new particle formation (NPF) is an important global phenomenon that is nevertheless sensitive to ambient conditions. According to both observation and theoretical arguments, NPF usually requires a relatively high sulfuric acid (H2SO4) concentration to promote the formation of new particles and a low preexisting aerosol loading to minimize the sink of new particles. We investigated NPF in Shanghai and were able to observe both precursor vapors (H2SO4) and initial clusters at a molecular level in a megacity. High NPF rates were observed to coincide with several familiar markers suggestive of H2SO4-dimethylamine (DMA)water (H2O) nucleation, including sulfuric acid dimers and H2SO4-DMA clusters. In a cluster kinetics simulation, the observed concentration of sulfuric acid was high enough to explain the particle growth to similar to 3 nanometers under the very high condensation sink, whereas the subsequent higher growth rate beyond this size is believed to result fromthe added contribution of condensing organic species. These findings will help in understanding urban NPF and its air quality and climate effects, as well as in formulating policies to mitigate secondary particle formation in China.
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