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- 2019
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Tidskriftsartikel (refereegranskat)
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| 2. |
- Guo, Yijie, et al.
(författare)
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Decreased expression of CHST-12, CHST-13, and UST in the proximal interphalangeal joint cartilage of school-age children with Kashin-Beck disease : an endemic osteoarthritis in China caused by selenium deficiency
- 2019
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Ingår i: Biological Trace Element Research. - New York : Springer. - 0163-4984 .- 1559-0720. ; 191:2, s. 276-285
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Tidskriftsartikel (refereegranskat)abstract
- The objective of this study is to investigate changes in the expression of enzymes involved in chondroitin sulfate (CS) sulfation in distal articular surface of proximal interphalangeal joint isolated from school-age children patients with Kashin-Beck disease (KBD), using normal children as controls. Articular cartilage samples were collected from four normal and four KBD children (7-12 years old), and these children were assigned to control and KBD groups. Hematoxylin and eosin (H&E), toluidine blue (TB), and immunohistochemical (IHC) stainings were utilized to evaluate changes in joint pathology and expression of enzymes involved in CS sulfation, including carbohydrate sulfotransferase 12 (CHST-12), carbohydrate sulfotransferase 13 (CHST-13), and uronyl 2-O-sulfotransferase (UST). The correspondence results were examined by semi-quantitative analysis. Compared with the control group, the KBD group showed the following: a significant decrease of total chondrocytes in superficial, middle, and deep layers and deposition of sulfated glycosaminoglycans in extracellular matrix of KBD cartilage were observed; positive staining chondrocytes of CHST-12, CHST-13, and UST were significantly less in superficial zone of KBD cartilage; and CHST-13 positive staining chondrocytes was reduced in deep zone of KBD cartilage. In contrast, the positive staining rates of CHST-12, CHST-13, and UST in KBD were significantly higher than those in the control group. The decreased expression of these enzymes and the physiologic compensatory reaction may be the signs of early-stage KBD. The alterations of CS structure modifying sulfotransferases in finger articular cartilage might play an important role in the onset and pathogenesis of school-age KBD children.
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| 3. |
- Lei, Jian, et al.
(författare)
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Abnormal expression of chondroitin sulfate sulfotransferases in the articular cartilage of pediatric patients with Kashin-Beck disease
- 2020
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Ingår i: Histochemistry and Cell Biology. - : Springer Science and Business Media LLC. - 0948-6143 .- 1432-119X. ; 153:3, s. 153-164
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Tidskriftsartikel (refereegranskat)abstract
- The objective of this study is to investigate the expression of enzymes involved in the sulfation of articular cartilage from proximal metacarpophalangeal (PMC) joint cartilage and distal metacarpophalangeal (DMC) joint cartilage in children with Kashin-Beck disease (KBD). The finger cartilage samples of PMC and DMC were collected from KBD and normal children aged 5-14 years old. Hematoxylin and eosin staining as well as immunohistochemical staining were used to observe the morphology and quantitate the expression of carbohydrate sulfotransferase 3 (CHST-3), carbohydrate sulfotransferase 12 (CHST-12), carbohydrate sulfotransferase 13 (CHST-13), uronyl 2-O-sulfotransferase (UST), and aggrecan. In the results, the numbers of chondrocyte decreased in all three zones of PMC and DMC in the KBD group. Less positive staining cells for CHST-3, CHST-12, CHST-13, UST, and aggrecan were observed in almost all three zones of PMC and DMC in KBD. The positive staining cell rates of CHST-12 were higher in superficial and middle zones of PMC and DMC in KBD, and a significantly higher rate of CHST-13 was observed only in superficial zone of PMC in KBD. In conclusion, the abnormal expression of chondroitin sulfate sulfotransferases in chondrocytes of KBD children may provide an explanation for the cartilage damage, and provide therapeutic targets for the treatment.
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| 4. |
- Pang, Xiaoying, et al.
(författare)
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The Present-day Mass Function of Star Clusters in the Solar Neighborhood
- 2024
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Ingår i: Astrophysical Journal. - 0004-637X .- 1538-4357. ; 966:2
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Tidskriftsartikel (refereegranskat)abstract
- This work analyzes the present-day mass function (PDMF) of 93 star clusters utilizing Gaia Data Release 3 data, with membership determined by the StarGo machine-learning algorithm. The impact of unresolved binary systems on mass estimation is rigorously assessed, adopting three mass ratio profiles for correction. The PDMF is characterized by the power-law index, α, derived through a robust maximum likelihood method that avoids biases associated with data binning. The value of α for stars between the completeness limited mass of Gaia (with a mean 0.3 M⊙ for our cluster samples) and 2 M⊙ exhibits stability for clusters younger than 200 Myr, decreasing for older clusters, particularly when considering stars within the half-mass radius. The PDMF of these star clusters is consistent with a dynamically evolved Kroupa initial mass function via the loss of low-mass stars. Cluster morphology shows a correlation with α, as α values exhibit a decreasing trend from filamentary to tidal-tail clusters, mirroring the sequence of increasing cluster age. The dependence of α on the total cluster mass is weak, with a subtle increase for higher-mass clusters, especially outside the half-mass radius. We do not observe a correlation between α and the mean metallicity of the clusters. Younger clusters have lower metallicity compared to their older counterparts, which indicates that the older clusters might have migrated to the solar neighborhood from the inner disk. A comparison with numerical models incorporating a black hole population suggests the need for observations of distant, older, massive open clusters to determine whether or not they contain black holes.
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| 5. |
- Wang, Siqi, et al.
(författare)
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Adjusting Competitive Reaction to Control Nucleation and Growth of MnO2 for a High-Stress Output Electrochemical Actuator
- 2023
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Ingår i: ACS APPLIED ELECTRONIC MATERIALS. - : AMER CHEMICAL SOC. - 2637-6113. ; 5:9, s. 4836-4845
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Tidskriftsartikel (refereegranskat)abstract
- Manganese dioxide (MnO2) with biocompatibility has promising applications in low-voltage electrochemical actuators of implantable medical devices, which can convert electrical energy to mechanical motion. However, the unsatisfactory actuation strain restricts the generation of a larger output stress of MnO2 for practical application. Herein, a competitive reaction-driven-MnO2 (CRD-MnO2) nanorod network was fabricated on a nickel (Ni) thin-film substrate by adjusting the component molar ratios. We find that the competitive reaction between 3,4-ethylene-dioxythiophene (EDOT) polymerization and oxidation of Mn2+ controls the nucleation and growth behavior of MnO2. The variation in the electron environment, newly generated oxygen vacancies, and a higher content of structural water effectively improve the electroactivity of MnO2 and simultaneously cause more serious Jahn-Teller (JT) distortion of the crystal octahedrons. Thus, an excellent output performance simultaneously having a much higher actuating strain of 8.3% and an actuation stress of 390.1 MPa is generated during a redox reaction between Mn4+ and Mn3+ under 0-1 V. Moreover, the CRD-MnO2/Ni composite actuating films assembled on a 3D-printed resin model of a human hand with separated finger joints can perform smooth grasping and releasing actions, demonstrating a huge potential for in vitro rehabilitation exercises and implantability for people with finger dyskinesia. This work provides a strategy for actuator material fabrication by controlling a nucleation and growth process by adjusting a competitive reaction.
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