| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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| 3. |
- Yang, Chao-Tung, et al.
(författare)
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Implementation of a real-time network traffic monitoring service with network functions virtualization
- 2019
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Ingår i: Future generations computer systems. - : Elsevier. - 0167-739X .- 1872-7115. ; 93, s. 687-701
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Tidskriftsartikel (refereegranskat)abstract
- The Network Functions Virtualization (NFV) extends the functionality provided by Software-Defined Networking (SDN). It is a virtualization technology that aims to replace the functionality provided by traditional networking hardware using software solutions. Thereby, enabling cheaper and efficient network deployment and management. The use of NFV and SDN is anticipated to enhance the performance of Infrastructure-as-a-Service (IaaS) clouds. However, due to the presence of a large number of network devices in IaaS clouds offering a plethora of networked services, there is need to develop a traffic monitoring system for the efficient network. This paper proposes and validates an extensible SDN and NFV-enabled network traffic monitoring system. Using extensive experiments, we show that the proposed system can closely match the performance of traditional networks at cheaper costs and by adding more flexibility to network management tasks.
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| 4. |
- Pilipenco, Alina, et al.
(författare)
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Negligible risk of surface transmission of SARS-CoV-2 in public transportation
- 2023
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Ingår i: Journal of Travel Medicine. - : Oxford University Press. - 1195-1982 .- 1708-8305. ; 30:5
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Tidskriftsartikel (refereegranskat)abstract
- Background: Exposure to pathogens in public transport systems is a common means of spreading infection, mainly by inhaling aerosol or droplets from infected individuals. Such particles also contaminate surfaces, creating a potential surface-transmission pathway.Methods: A fast acoustic biosensor with an antifouling nano-coating was introduced to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on exposed surfaces in the Prague Public Transport System. Samples were measured directly without pre-treatment. Results with the sensor gave excellent agreement with parallel quantitative reverse-transcription polymerase chain reaction (qRT-PCR) measurements on 482 surface samples taken from actively used trams, buses, metro trains and platforms between 7 and 9 April 2021, in the middle of the lineage Alpha SARS-CoV-2 epidemic wave when 1 in 240 people were COVID-19 positive in Prague.Results: Only ten of the 482 surface swabs produced positive results and none of them contained virus particles capable of replication, indicating that positive samples contained inactive virus particles and/or fragments. Measurements of the rate of decay of SARS-CoV-2 on frequently touched surface materials showed that the virus did not remain viable longer than 1-4 h. The rate of inactivation was the fastest on rubber handrails in metro escalators and the slowest on hard-plastic seats, window glasses and stainless-steel grab rails. As a result of this study, Prague Public Transport Systems revised their cleaning protocols and the lengths of parking times during the pandemic.Conclusions: Our findings suggest that surface transmission played no or negligible role in spreading SARS-CoV-2 in Prague. The results also demonstrate the potential of the new biosensor to serve as a complementary screening tool in epidemic monitoring and prognosis.
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