| 1. |
- Beal, Jacob, et al.
(author)
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Robust estimation of bacterial cell count from optical density
- 2020
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In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Journal article (peer-reviewed)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Cheung, Ho-Nam, et al.
(author)
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Assessing the influence of sea surface temperature and arctic sea ice cover on the uncertainty in the boreal winter future climate projections
- 2022
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In: Climate Dynamics. - : Springer Science and Business Media LLC. - 0930-7575 .- 1432-0894. ; 59:1-2, s. 433-454
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Journal article (peer-reviewed)abstract
- We investigate the uncertainty (i.e., inter-model spread) in future projections of the boreal winter climate, based on the forced response of ten models from the CMIP5 following the RCP8.5 scenario. The uncertainty in the forced response of sea level pressure (SLP) is large in the North Pacific, the North Atlantic, and the Arctic. A major part of these uncertainties (31%) is marked by a pattern with a center in the northeastern Pacific and a dipole over the northeastern Atlantic that we label as the Pacific–Atlantic SLP uncertainty pattern (PA∆SLP). The PA∆SLP is associated with distinct global sea surface temperature (SST) and Arctic sea ice cover (SIC) perturbation patterns. To better understand the nature of the PA∆SLP, these SST and SIC perturbation patterns are prescribed in experiments with two atmospheric models (AGCMs): CAM4 and IFS. The AGCM responses suggest that the SST uncertainty contributes to the North Pacific SLP uncertainty in CMIP5 models, through tropical–midlatitude interactions and a forced Rossby wavetrain. The North Atlantic SLP uncertainty in CMIP5 models is better explained by the combined effect of SST and SIC uncertainties, partly related to a Rossby wavetrain from the Pacific and air-sea interaction over the North Atlantic. Major discrepancies between the CMIP5 and AGCM forced responses over northern high-latitudes and continental regions are indicative of uncertainties arising from the AGCMs. We analyze the possible dynamic mechanisms of these responses, and discuss the limitations of this work.
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| 3. |
- Jin, Shaobo, et al.
(author)
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A Marginal Maximum Likelihood Approach for Extended Quadratic Structural Equation Modeling with Ordinal Data
- 2020
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In: STRUCTURAL EQUATION MODELING: A MULTIDISCIPLINARY JOURNAL. - USA : Taylor & Francis Group. - 1070-5511 .- 1532-8007. ; 27:6, s. 864-873
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Journal article (peer-reviewed)abstract
- The literature on non-linear structural equation modeling is plentiful. Despite this fact, few studies consider interactions between exogenous and endogenous latent variables. Further, it is well known that treating ordinal data as continuous produces bias, a problem that is enhanced when non-linear relationships between latent variables are incorporated. A marginal maximum likelihood-based approach is proposed in order to fit a non-linear structural equation model, including interactions between exogenous and endogenous latent variables in the presence of ordinal data. In this approach, the exact gradient of the approximated observed log-likelihood is calculated in order to attain the approximated maximum likelihood estimator. A simulation study shows that the proposed method provides estimates with low bias and accurate coverage probabilities
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| 4. |
- Jin, Shaobo, et al.
(author)
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A Simulation Study of Polychoric Instrumental Variable Estimation in Structural Equation Models
- 2016
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In: Structural Equation Modeling. - : Informa UK Limited. - 1070-5511 .- 1532-8007. ; 23:5, s. 680-694
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Journal article (peer-reviewed)abstract
- Data collected from questionnaires are often in ordinal scale. Unweighted least squares (ULS), diagonally weighted least squares (DWLS) and normal-theory maximum likelihood (ML) are commonly used methods to fit structural equation models. Consistency of these estimators demands no structural misspecification. In this article, we conduct a simulation study to compare the equation-by-equation polychoric instrumental variable (PIV) estimation with ULS, DWLS, and ML. Accuracy of PIV for the correctly specified model and robustness of PIV for misspecified models are investigated through a confirmatory factor analysis (CFA) model and a structural equation model with ordinal indicators. The effects of sample size and nonnormality of the underlying continuous variables are also examined. The simulation results show that PIV produces robust factor loading estimates in the CFA model and in structural equation models. PIV also produces robust path coefficient estimates in the model where valid instruments are used. However, robustness highly depends on the validity of instruments.
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| 5. |
- Jin, Shaobo, 1987-, et al.
(author)
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A unified model-implied instrumental variable approach for structural equation modeling with mixed variables
- 2021
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In: Psychometrika. - : Springer Nature. - 0033-3123 .- 1860-0980. ; 86:2, s. 564-594
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Journal article (peer-reviewed)abstract
- The model-implied instrumental variable (MIIV) estimator is an equation-by-equation estimator of structural equation models that is more robust to structural misspecifications than full information estimators. Previous studies have concentrated on endogenous variables that are all continuous (MIIV-2SLS) or all ordinal . We develop a unified MIIV approach that applies to a mixture of binary, ordinal, censored, or continuous endogenous observed variables. We include estimates of factor loadings, regression coefficients, variances, and covariances along with their asymptotic standard errors. In addition, we create new goodness of fit tests of the model and overidentification tests of single equations. Our simulation study shows that the proposed MIIV approach is more robust to structural misspecifications than diagonally weighted least squares (DWLS) and that both the goodness of fit model tests and the overidentification equations tests can detect structural misspecifications. We also find that the bias in asymptotic standard errors for the MIIV estimators of factor loadings and regression coefficients are often lower than the DWLS ones, though the differences are small in large samples. Our analysis shows that scaling indicators with low reliability can adversely affect the MIIV estimators. Also, using a small subset of MIIVs reduces small sample bias of coefficient estimates, but can lower the power of overidentification tests of equations.
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| 6. |
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| 7. |
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| 8. |
- Jin, Shaobo, 1987-, et al.
(author)
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Approximated penalized maximum likelihood for exploratory factor analysis : An orthogonal case
- 2018
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In: Psychometrika. - : Springer Science and Business Media LLC. - 0033-3123 .- 1860-0980. ; 83:3, s. 628-649
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Journal article (peer-reviewed)abstract
- The problem of penalized maximum likelihood (PML) for an exploratory factor analysis (EFA) model is studied in this paper. An EFA model is typically estimated using maximum likelihood and then the estimated loading matrix is rotated to obtain a sparse representation. Penalized maximum likelihood simultaneously fits the EFA model and produces a sparse loading matrix. To overcome some of the computational drawbacks of PML, an approximation to PML is proposed in this paper. It is further applied to an empirical dataset for illustration. A simulation study shows that the approximation naturally produces a sparse loading matrix and more accurately estimates the factor loadings and the covariance matrix, in the sense of having a lower mean squared error than factor rotations, under various conditions.
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| 9. |
- Jin, Shaobo, et al.
(author)
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Asymptotic Efficiency of the Pseudo-Maximum Likelihood Estimator in Multi-Group Factor Models with Pooled Data
- 2016
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In: British Journal of Mathematical & Statistical Psychology. - : Wiley. - 0007-1102 .- 2044-8317. ; 69:1, s. 20-42
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Journal article (peer-reviewed)abstract
- A multi-group factor model is suitable for data originating from different strata. However, it often requires a relatively large sample size to avoid numerical issues such as non-convergence and non-positive definite covariance matrices. An alternative is to pool data from different groups in which a single-group factor model is fitted to the pooled data using maximum likelihood. In this paper, properties of pseudo-maximum likelihood (PML) estimators for pooled data are studied. The pooled data are assumed to be normally distributed from a single group. The resulting asymptotic efficiency of the PML estimators of factor loadings is compared with that of the multi-group maximum likelihood estimators. The effect of pooling is investigated through a two-group factor model. The variances of factor loadings for the pooled data are underestimated under the normal theory when error variances in the smaller group are larger. Underestimation is due to dependence between the pooled factors and pooled error terms. Small-sample properties of the PML estimators are also investigated using a Monte Carlo study.
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| 10. |
- Jin, Shaobo, 1987-, et al.
(author)
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Asymptotic Robustness Study Of The Polychoric Correlation Estimation
- 2017
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In: Psychometrika. - : Springer Science and Business Media LLC. - 0033-3123 .- 1860-0980. ; 82:1, s. 67-85
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Journal article (peer-reviewed)abstract
- Asymptotic robustness against misspecification of the underlying distribution for the polychoric correlation estimation is studied. The asymptotic normality of the pseudo-maximum likelihood estimator is derived using the two-step estimation procedure. The t distribution assumption and the skew-normal distribution assumption are used as alternatives to the normal distribution assumption in a numerical study. The numerical results show that the underlying normal distribution can be substantially biased, even though skewness and kurtosis are not large. The skew-normal assumption generally produces a lower bias than the normal assumption. Thus, it is worth using a non-normal distributional assumption if the normal assumption is dubious.
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