| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Kristanl, Matej, et al.
(författare)
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The Seventh Visual Object Tracking VOT2019 Challenge Results
- 2019
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Ingår i: 2019 IEEE/CVF INTERNATIONAL CONFERENCE ON COMPUTER VISION WORKSHOPS (ICCVW). - : IEEE COMPUTER SOC. - 9781728150239 ; , s. 2206-2241
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking challenge VOT2019 is the seventh annual tracker benchmarking activity organized by the VOT initiative. Results of 81 trackers are presented; many are state-of-the-art trackers published at major computer vision conferences or in journals in the recent years. The evaluation included the standard VOT and other popular methodologies for short-term tracking analysis as well as the standard VOT methodology for long-term tracking analysis. The VOT2019 challenge was composed of five challenges focusing on different tracking domains: (i) VOT-ST2019 challenge focused on short-term tracking in RGB, (ii) VOT-RT2019 challenge focused on "real-time" short-term tracking in RGB, (iii) VOT-LT2019 focused on long-term tracking namely coping with target disappearance and reappearance. Two new challenges have been introduced: (iv) VOT-RGBT2019 challenge focused on short-term tracking in RGB and thermal imagery and (v) VOT-RGBD2019 challenge focused on long-term tracking in RGB and depth imagery. The VOT-ST2019, VOT-RT2019 and VOT-LT2019 datasets were refreshed while new datasets were introduced for VOT-RGBT2019 and VOT-RGBD2019. The VOT toolkit has been updated to support both standard short-term, long-term tracking and tracking with multi-channel imagery. Performance of the tested trackers typically by far exceeds standard baselines. The source code for most of the trackers is publicly available from the VOT page. The dataset, the evaluation kit and the results are publicly available at the challenge website(1).
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| 3. |
- Deng, Huan, et al.
(författare)
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Progress of selenium deficiency in the pathogenesis of arthropathies and selenium supplement for their treatment
- 2022
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Ingår i: Biological Trace Element Research. - : Springer Nature. - 0163-4984 .- 1559-0720. ; 200, s. 4238-4249
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Forskningsöversikt (refereegranskat)abstract
- Selenium, an essential trace element for human health, exerts an indispensable effect in maintaining physiological homeostasis and functions in the body. Selenium deficiency is associated with arthropathies, such as Kashin-Beck disease, rheumatoid arthritis, osteoarthritis, and osteoporosis. Selenium deficiency mainly affects the normal physiological state of bone and cartilage through oxidative stress reaction and immune reaction. This review aims to explore the role of selenium deficiency and its mechanisms existed in the pathogenesis of arthropathies. Meanwhile, this review also summarized various experiments to highlight the crucial functions of selenium in maintaining the homeostasis of bone and cartilage.
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| 4. |
- Gao, Hongkai, et al.
(författare)
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Stepwise modeling and the importance of internal variables validation to test model realism in a data scarce glacier basin
- 2020
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Ingår i: Journal of Hydrology. - : Elsevier BV. - 0022-1694. ; 591
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Tidskriftsartikel (refereegranskat)abstract
- Model realism is of vital importance in science of hydrology, in terms of realistic representation of hydrological processes and reliability of future prediction. Here, we employed a stepwise modeling approach that leverages flexible model structures and multi-source observations for robust streamflow simulation and internal variables validation with improved model realism. This framework is demonstrated in Yigong Zangbu River (YZR) basin, a data scarce glacier basin in the upper Brahmaputra River. We designed six experiments (Exp1–6) to use modeling as a tool to understand hydrological processes in this remote cold basin with extremely high altitude. In Exp1, we started with a distributed rainfall-runoff model (FLEXD) - representing the case that snow and glacier processes were ignored. Then, we stepwisely added snow and glacier processes into FLEXD, denoted as FLEXD-S (Exp2) and FLEXD-SG (Exp3), respectively, and such improvement of model structure led to significantly improved streamflow estimates. To explore the impact of different precipitation forcing on model performance, FLEXD-SG was driven by Theissen average (Exp3) and three individual stations’ precipitation (Exp4–6). The model realism was tested by observed hydrograph, snow cover area (SCA) and glacier mass balance (GMB). Results showed that a robust and realistic hydrological modeling system was achieved in Exp6. In this modeling study, we learned that: 1) stepwise modeling is effective in investigating catchment behavior, and snow and glacier melting are the dominant hydrological processes in the YZR basin; 2) internal variables validation is beneficial to test model realism in data scarce basin; 3) the FLEXD-SG model calibrated by only one year hydrograph is sufficient to reproduce snow and glacier variations; 4) precipitation of a single station as forcing data could outperform Theissen average; 5) based on the well tested model configuration in Exp6, we analyzed simulated results, and reconstructed the long term hydrography (1961–2013), to support the potential competence for decision making on water resources management in practice. The proposed framework may significantly improve our skills in hydrological modeling over data-poor regions.
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| 5. |
- Li, Wenxiong, et al.
(författare)
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Observations of Type Ia Supernova 2014J for Nearly 900 Days and Constraints on Its Progenitor System
- 2019
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Ingår i: Astrophysical Journal. - : American Astronomical Society. - 0004-637X .- 1538-4357. ; 882:1
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Tidskriftsartikel (refereegranskat)abstract
- We present extensive ground-based and Hubble Space Telescope (HST) photometry of the highly reddened, very nearby SN Ia 2014J in M82, covering the phases from 9 days before to about 900 days after the B-band maximum. SN 2014J is similar to other normal SNe Ia near the maximum light, but it shows flux excess in the B band in the early nebular phase. This excess flux emission can be due to light scattering by some structures of circum stellar materials located at a few 10(17) cm, consistent with a single-degenerate progenitor system or a double-degenerate progenitor system with mass outflows in the final evolution or magnetically driven winds around the binary system. At t similar to +300 to similar to +500 days past the B-band maximum, the light curve of SN 2014J shows a faster decline relative to the Ni-56 decay. That feature can be attributed to the significant weakening of the emission features around [Fe III] lambda 4700 and [Fe II] lambda 5200 rather than the positron escape, as previously suggested. Analysis of the HST images taken at t > 600 days confirms that the luminosity of SN 2014J maintains a flat evolution at the very late phase. Fitting the late-time pseudobolometric light curve with radioactive decay of Ni-56, Ni-57, and Fe-55 isotopes, we obtain the mass ratio Ni-57/Ni-56 as 0.035 +/- 0.011, which is consistent with the corresponding value predicted from the 2D and 3D delayed-detonation models. Combined with early-time analysis, we propose that delayed detonation through the single-degenerate scenario is most likely favored for SN 2014J.
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| 6. |
- Liu, Quan, et al.
(författare)
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Deep reinforcement learning-based safe interaction for industrial human-robot collaboration using intrinsic reward function
- 2021
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Ingår i: Advanced Engineering Informatics. - : Elsevier BV. - 1474-0346 .- 1873-5320. ; 49
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Tidskriftsartikel (refereegranskat)abstract
- Aiming at human-robot collaboration in manufacturing, the operator's safety is the primary issue during the manufacturing operations. This paper presents a deep reinforcement learning approach to realize the real-time collision-free motion planning of an industrial robot for human-robot collaboration. Firstly, the safe human robot collaboration manufacturing problem is formulated into a Markov decision process, and the mathematical expression of the reward function design problem is given. The goal is that the robot can autonomously learn a policy to reduce the accumulated risk and assure the task completion time during human-robot collaboration. To transform our optimization object into a reward function to guide the robot to learn the expected behaviour, a reward function optimizing approach based on the deterministic policy gradient is proposed to learn a parameterized intrinsic reward function. The reward function for the agent to learn the policy is the sum of the intrinsic reward function and the extrinsic reward function. Then, a deep reinforcement learning algorithm intrinsic reward-deep deterministic policy gradient (IRDDPG), which is the combination of the DDPG algorithm and the reward function optimizing approach, is proposed to learn the expected collision avoidance policy. Finally, the proposed algorithm is tested in a simulation environment, and the results show that the industrial robot can learn the expected policy to achieve the safety assurance for industrial human-robot collaboration without missing the original target. Moreover, the reward function optimizing approach can help make up for the designed reward function and improve policy performance.
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| 7. |
- Yang, Liu, et al.
(författare)
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Meter-scale transparent conductive circuits based on silver nanowire networks for rigid and flexible transparent light-emitting diode screens
- 2019
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Ingår i: Optical Materials Express. - : Optical Society of America. - 2159-3930 .- 2159-3930. ; 9:12, s. 4483-4496
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Tidskriftsartikel (refereegranskat)abstract
- Meter-scale transparent conductive circuits based on silver nanowire (AgNW) networks are fabricated for transparent light-emitting diode (LED) screens on both rigid and flexible substrates. A 25-cm long AgNW transparent conductive strip is fabricated with a strip resistivity of 9.95 Omega/cm. A high uniformity is achieved in terms of film optical transmission (up to 84.5% in average) and sheet resistance (as low as 4.7 Omega/sq in average), superior to ITO. A transparent LED screen based on a 1.2-m ultralong AgNW circuit is demonstrated with LEDs emitting bright red, green and blue lights under different biases. The AgNW strip on a polyethylene terephthalate substrate shows mechanical flexibility and stable performance in bending tests. Based on this, a flexible transparent LED screen is proposed and presented. It works well when dynamically bent to a radius as small as similar to 15 mm. Therefore, the AgNW transparent conductive circuits are very promising as a replacement to ITO circuits for such smart screens, to be integrated into modern glass architectures and display videos in various public places.
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| 8. |
- Yang, Zhihao, et al.
(författare)
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Characteristics of health-state utilities used in cost-effectiveness analyses : a systematic review of published studies in Asia
- 2023
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Ingår i: Health and Quality of Life Outcomes. - : BioMed Central (BMC). - 1477-7525. ; 21:1
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Forskningsöversikt (refereegranskat)abstract
- Introduction: Cost-utility analysis (CUA) is the preferred form of economic evaluation in many countries. As one of the key data inputs in cost-utility models, health state utility (HSU) has a crucial impact on CUA results. In the past decades, health technology assessment has been expanding rapidly in Asia, yet research examining the methodology and process used to generate cost-effectiveness evidence is scarce. The aim of this study was to examine the reporting of the characteristics of HSU data used in CUAs in Asia and how the characteristics have changed over time.Methods: A systematic literature search was performed to identify published CUA studies targeting Asian populations. Information was extracted for both the general characteristics of selected studies and the characteristics of reported HSU data. For each HSU value identified, we extracted data for four key characteristics, including 1) estimation method; 2) source of health-related quality of life (HRQoL) data; 3) source of preference data; and 4) sample size. The percentage of nonreporting was calculated and compared over two time periods (1990–2010 vs 2011–2020).Results: A total of 789 studies were included and 4,052 HSUs were identified. Of these HSUs, 3,351 (82.7%) were from published literature and 656 (16.2%) were from unpublished empirical data. Overall, the characteristics of HSU data were not reported in more than 80% of the studies. Of HSUs whose characteristics were reported, most of them were estimated using the EQ-5D (55.7%), Asian HRQoL data (91.9%), and Asian health preferences (87.7%); 45.7% of the HSUs was estimated with a sample of 100 or more individuals. All four characteristics showed improvements after 2010.Conclusion: Over the past two decades, there has been a significant increase in CUA studies targeting Asian populations. However, HSU’s characteristics were not reported in most of the CUA studies, making it difficult to evaluate the quality and appropriateness of the HSUs used in those cost-effectiveness studies.
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