| 1. |
- Gui, Xinru, et al.
(författare)
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Identification and validation of volatile organic compounds in bile for differential diagnosis of perihilar cholangiocarcinoma
- 2023
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Ingår i: Clinica Chimica Acta. - : Elsevier. - 0009-8981 .- 1873-3492. ; 541
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Tidskriftsartikel (refereegranskat)abstract
- Early and differential diagnosis of perihilar cholangiocarcinoma (PHCCA) is highly challenging. This study aimed to evaluate whether volatile organic compounds (VOCs) in bile samples could be emerging diagnostic biomarkers for PHCCA. We collected 200 bile samples from patients with PHCCA and benign biliary diseases (BBD), including a 140-patient training cohort and an 60-patient test cohort. Gas chromatography-ion mobility spec-trometry (GC-IMS) was used for VOCs detection. The predictive models were constructed using machine learning algorithms. Our analysis detected 19 VOC substances using GC-IMS in the bile samples and resulted in the identification of three new VOCs, 2-methoxyfuran, propyl isovalerate, and diethyl malonate that were found in bile. Unsupervised hierarchical clustering analysis supported that VOCs detected in the bile could distinguish PHCCA from BBD. Twelve VOCs defined according to 32 signal peaks had significant statistical significance between BBD and PHCCA, including four up-regulated VOCs in PHCCA, such as 2-ethyl-1-hexanol, propyl iso-valerate, cyclohexanone, and acetophenone, while the rest eight VOCs were down-regulated. ROC curve analysis revealed that machine learning models based on VOCs could help diagnosing PHCCA. Among them, SVM pro-vided the highest AUC of 0.966, with a sensitivity and specificity of 93.1% and 100%, respectively. The diag-nostic model based on different VOC spectra could be a feasible method for the differential diagnosis of PHCCA.
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| 2. |
- Chen, Yulong, et al.
(författare)
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Hypercysteinemia promotes atherosclerosis by reducing protein S-nitrosylation.
- 2015
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Ingår i: Biomedicine and Pharmacotherapy. - : Elsevier BV. - 1950-6007 .- 0753-3322. ; 70, s. 253-259
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Tidskriftsartikel (refereegranskat)abstract
- Protein S-nitrosylation plays important role in the regulation of cardiovascular functions in nitric oxide (NO) Pathway. Hypercysteinemia (HHcy) is an independently risk factor for atherosclerosis. We hypothesized that HHcy promotes atherosclerosis by reducing level of vascular protein S-nitrosylation. The aim of present study is to investigate effect of HHcy on vascular protein S-nitrosylation. A total of 45 male apoE-/- mice were randomly divided into three groups. The control group was fed a Western-type diet. The HHcy group was fed a diet containing 4.4% l-methionine, and the HHcy+NONOate group was fed a diet containing 4.4% l-methionine and administrated NONOate (ip). Human umbilical vein endothelial cells were performed for in vitro experiment. Plasma lipids were measured every 4 weeks. After 12 weeks, aortic atherosclerotic lesion areas were detected as well as cellular components. The levels of plasma homocysteine (Hcy) and NO were measured. S-nitrosylation was detected using immunofluorescence, and further confirmed by biotin switch method. We found that compared with the control group, Hcy levels, and atherosclerotic plaque, and content of vascular smooth muscle cells and macrophages in lesions significantly increased, and levels of NO significantly decreased in the HHcy group. However, NONOate reverses this effect. In addition, Hcy significantly reduced protein S-nitrosylation in human umbilical vein endothelial cells. This reduction of protein S-nitrosylation was accompanied by reduced levels of NO. Our results suggested that Hcy promoted atherosclerosis by inhibiting vascular protein S-nitrosylation.
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| 3. |
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| 4. |
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| 5. |
- Kang, Naixin, et al.
(författare)
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Antischistosomal Properties of Hederacolchiside A1 Isolated from Pulsatilla chinensis
- 2018
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Ingår i: Molecules. - : MDPI. - 1431-5157 .- 1420-3049. ; 23:6
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Tidskriftsartikel (refereegranskat)abstract
- Background: Schistosomiasis is a major neglected disease for which the current control strategy involves mass treatment with praziquantel, the only available drug. Hence, there is an urgent need to develop new antischistosomal compounds.Methods: The antischistosomal activity of hederacolchiside A1 (HSA) were determined by total or female worm burden reductions in mice harboring Schistosoma japonicum or S. mansoni. Pathology parameters were detected on HSA against 1-day-old S. japonicum-harboring mice. Moreover, we confirmed the antischistosomal effect of HSA on newly transformed schistosomula (NTS) of S. japonicum in vitro.Results: HSA, a natural product isolated from Pulsatilla chinensis (Bunge) Regel, was initially corroborated to possess promising antischistosomal properties. We demonstrated that HSA had high activity against S. japonicum and S. mansoni less in 11 days old parasites harbored in mice. The antischistosomal effect was even more than the currently used drugs, praziquantel, and artesunate. Furthermore, HSA could ameliorate the pathology parameters in mice harboring 1-day-old juvenile S. japonicum. We also confirmed that HSA-mediated antischistosomal activity is partly due to the morphological changes in the tegument system when NTS are exposed to HSA.Conclusions: HSA may have great potential to be an antischistosomal agent for further research.
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| 6. |
- Kouveliotis Lysikatos, Iasonas, 1989-, et al.
(författare)
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Towards an Internet-like Power Grid
- 2022
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Ingår i: Journal of Modern Power Systems and Clean Energy. - : Journal of Modern Power Systems and Clean Energy. - 2196-5625 .- 2196-5420. ; 10:1, s. 1-11
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Forskningsöversikt (refereegranskat)abstract
- The great challenges faced by modern power systems require a fresh look at the conventional operation paradigm. The significant challenges faced by modern power systems require an innovative method for the conventional operation paradigm. We claim that the decarbonization of the power grid and extensive electrification of numerous sectors of human activity can only be fostered by a self-adaptable and smart power grid that manifests similar qualities to those of the Internet. The Internet is constructed on a layered architecture that facilitates technology innovations and its intelligence is distributed throughout a hierarchy of networks. In this paper, the fundamental differences between the network data flows and power flows are examined, and the basic requirements for an innovative operation paradigm are highlighted. The current power grid is operated in a highly inflexible, centralized manner to meet increased security goals. A new highly flexible, distributed architecture can be realized by distributing the operation responsibility in smaller areas or even in grid components that can make autonomous decisions. The characteristics of such a power grid are presented, and the key features and advances for the on-going transition to a sustainable power system are identified. Finally, a case study on distributed voltage control is presented and discussed.
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| 7. |
- Li, Pei-Zhou, et al.
(författare)
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A highly porous metal-organic framework for large organic molecule capture and chromatographic separation
- 2017
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Ingår i: Chemical Communications. - : Royal Society of Chemistry (RSC). - 1359-7345 .- 1364-548X. ; 53:24, s. 3434-3437
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Tidskriftsartikel (refereegranskat)abstract
- A highly porous metal-organic framework (MOF) with large pores was successfully obtained via solvothermal assembly of a ''click''-extended tricarboxylate ligand and Zn(II) ions. The inherent feature of large-molecule accessible pores endows the MOF with a unique property for utilization toward large guest molecules.
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| 8. |
- Li, Shilei, 1982, et al.
(författare)
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Evaluation of hardening behaviors in ion-irradiated Fe–9Cr and Fe–20Cr alloys by nanoindentation technique
- 2016
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Ingår i: Journal of Nuclear Materials. - : Elsevier BV. - 0022-3115. ; 478, s. 50-56
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Tidskriftsartikel (refereegranskat)abstract
- The ion irradiation hardening behaviors of Fee9 wt% Cr and Fee20 wt% Cr model alloys were investigatedby nanoindentation technique. The specimens were irradiated with 3 MeV Fe11þ ions at roomtemperature up to 1 and 5 dpa for Fee9Cr alloy and 1 and 2.5 for Fee20Cr alloy. The ratio of averagehardness in the same depth of irradiated and unirradiated (Hirr. av/Hunirr. av) was used to determine thecritical indentation depth hcrit to eliminate the softer substrate effect. The NixeGao model was used toexplain the indentation size effect. Irradiation hardening is clearly observed in both Fee9Cr alloy and Fee20Cr alloy after ion irradiation. The differences of ISE and irradiation hardening behaviors between Fee9Cr and Fee20Cr alloys are considered to be due to their different microstructures and microstructuralevolution under ion irradiation.
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| 9. |
- Liu, Guofeng, et al.
(författare)
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Helicity Inversion of Supramolecular Hydrogels Induced by Achiral Substituents
- 2017
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Ingår i: ACS Nano. - : American Chemical Society (ACS). - 1936-0851 .- 1936-086X. ; 11:12, s. 11880-11889
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Tidskriftsartikel (refereegranskat)abstract
- Probing the supramolecular chirality of assemblies and controlling their handedness are closely related to the origin of chirality at the supramolecular level and the development of smart materials with desired handedness. However, it remains unclear how achiral residues covalently bonded to chiral amino acids can function in the chirality inversion of supramolecular assemblies. Herein, we report macroscopic chirality and dynamic manipulation of chiroptical activity of hydrogels self-assembled from phenylalanine derivatives, together with the inversion of their handedness achieved solely by exchanging achiral substituents between oligo(ethylene glycol) and carboxylic acid groups. This helicity inversion is mainly induced by distinct stacking mode of the self-assembled building blocks, as collectively confirmed by scanning electron microscopy, circular dichroism, crystallography, and molecular dynamics calculations. Through this straightforward approach, we were able to invert the handedness of helical assemblies by merely exchanging achiral substituents at the terminal of chiral gelators. This work not only presents a feasible strategy to achieve the handedness inversion of helical nanostructures for better understanding of chiral self-assembly process in supramolecular chemistry but also facilities the development of smart materials with controllable handedness in materials science.
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| 10. |
- Liu, Yanli, et al.
(författare)
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Towards a limits of acceptability approach to the calibration of hydrological models : Extending observation error
- 2009
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Ingår i: Journal of Hydrology. - : Elsevier BV. - 0022-1694 .- 1879-2707. ; 367:1-2, s. 93-103
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Tidskriftsartikel (refereegranskat)abstract
- Within the GLUE methodology, there are a number of advantages of taking a limits of acceptability approach to model evaluation for non-ideal applications where the strong assumptions of statistical identification might be difficult to justify. However, there is a question of how the limits of acceptability might be specified in a way that reflects the different Sources of uncertainty in the modeling process. Here, a novel method for identifying behavioural models in an extended GLUE methodology is developed and applied to an application of Dynamic TOPMODEL to the Attert catchment in Luxemburg with semi-distributed inputs to nested sub-catchments. The results raise some important issues about testing model structures as hypotheses of catchment responses. (C) 2009 Elsevier B.V. All rights reserved.
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