| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Barbette, Tom, 1990-, et al.
(författare)
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A High-Speed Load-Balancer Design with Guaranteed Per-Connection-Consistency
- 2020
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Ingår i: Proceedings of the 17th USENIX Symposium on Networked Systems Design and Implementation, NSDI 2020. - Santa Clara, CA, USA : USENIX Association. ; , s. 667-683
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Konferensbidrag (refereegranskat)abstract
- Large service providers use load balancers to dispatch millions of incoming connections per second towards thousands of servers. There are two basic yet critical requirements for a load balancer: uniform load distribution of the incoming connections across the servers and per-connection-consistency (PCC), i.e., the ability to map packets belonging to the same connection to the same server even in the presence of changes in the number of active servers and load balancers. Yet, meeting both these requirements at the same time has been an elusive goal. Today's load balancers minimize PCC violations at the price of non-uniform load distribution.This paper presents Cheetah, a load balancer that supports uniform load distribution and PCC while being scalable, memory efficient, resilient to clogging attacks, and fast at processing packets. The Cheetah LB design guarantees PCC for any realizable server selection load balancing mechanism and can be deployed in both a stateless and stateful manner, depending on the operational needs. We implemented Cheetah on both a software and a Tofino-based hardware switch. Our evaluation shows that a stateless version of Cheetah guarantees PCC, has negligible packet processing overheads, and can support load balancing mechanisms that reduce the flow completion time by a factor of 2–3×.
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| 3. |
- Wang, Xi, et al.
(författare)
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Self-Constructed Multiple Plasmonic Hotspots on an Individual Fractal to Amplify Broadband Hot Electron Generation
- 2021
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Ingår i: ACS Nano. - : American Chemical Society (ACS). - 1936-0851 .- 1936-086X. ; 15:6, s. 10553-10564
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Tidskriftsartikel (refereegranskat)abstract
- Plasmonic nanoparticles are ideal candidates for hot-electron-assisted applications, but their narrow resonance region and limited hotspot number hindered the energy utilization of broadband solar energy. Inspired by tree branches, we designed and chemically synthesized silver fractals, which enable self-constructed hotspots and multiple plasmonic resonances, extending the broadband generation of hot electrons for better matching with the solar radiation spectrum. We directly revealed the plasmonic origin, the spatial distribution, and the decay dynamics of hot electrons on the single-particle level by using ab initio simulation, dark-field spectroscopy, pump–probe measurements, and electron energy loss spectroscopy. Our results show that fractals with acute tips and narrow gaps can support broadband resonances (400–1100 nm) and a large number of randomly distributed hotspots, which can provide unpolarized enhanced near field and promote hot electron generation. As a proof-of-concept, hot-electron-triggered dimerization of p-nitropthiophenol and hydrogen production are investigated under various irradiations, and the promoted hot electron generation on fractals was confirmed with significantly improved efficiency.
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