| 2. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 3. |
- Park, Kwangseok, et al.
(författare)
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Avalanche Coalescence of Liquid Metal Particles for Uniform Flexible and Stretchable Electrodes
- 2022
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Ingår i: Advanced Materials Interfaces. - : John Wiley & Sons. - 2196-7350. ; 9:35
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Tidskriftsartikel (refereegranskat)abstract
- Self-sinterable gallium-based liquid metal (LM) ink for a handy drop-casting coating method is developed. The fabricated LM electrode is coffee-ring-free and bilayer-free, which guarantees high electrical conductivity, wettability, flexibility, and stretchability. The ink consists of Galinstan, ethanol-water mixture, hydrochloric acid, and polyvinylpyrrolidone. The binary mixture provides excellent wetting conditions and suppresses a coffee-ring stain from Marangoni mixing. The acid removes an oxide layer on the LM interface. The colloidal interaction forces by self-assembled polyvinylpyrrolidone induce avalanche coalescences of the particles at the end of evaporation, which produces a thin and uniform dried conductive layer. The acid and polymer provide a bilayer-free structure and electrification without any post sintering. The drop-casted self-transformed LM electrode showed excellent electrical resistance of O (0.1-1 omega) on folding and stretching conditions. It is believed that this method can serve as flexible and stretchable electrodes, on various substances for soft robots and wearable devices, without any complicated post-process.
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