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- Liljeruhm, Josefine, et al.
(författare)
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Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology
- 2018
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Ingår i: Journal of Biological Engineering. - : BIOMED CENTRAL LTD. - 1754-1611. ; 12
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Tidskriftsartikel (refereegranskat)abstract
- Background: Coral reefs are colored by eukaryotic chromoproteins (CPs) that are homologous to green fluorescent protein. CPs differ from fluorescent proteins (FPs) by intensely absorbing visible light to give strong colors in ambient light. This endows CPs with certain advantages over FPs, such as instrument-free detection uncomplicated by ultra-violet light damage or background fluorescence, efficient Forster resonance energy transfer (FRET) quenching, and photoacoustic imaging. Thus, CPs have found utility as genetic markers and in teaching, and are attractive for potential cell biosensor applications in the field. Most near-term applications of CPs require expression in a different domain of life: bacteria. However, it is unclear which of the eukaryotic CP genes might be suitable and how best to assay them.Results: Here, taking advantage of codon optimization programs in 12 cases, we engineered 14 CP sequences (meffRed, eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, mefiBlue, aeBlue, amilCP, tsPurple and gfasPurple) into a palette of Escherichia coil BioBrick plasmids. BioBricks comply with synthetic biology's most widely used, simplified, cloning standard. Differences in color intensities, maturation times and fitness costs of expression were compared under the same conditions, and visible readout of gene expression was quantitated. A surprisingly large variation in cellular fitness costs was found, resulting in loss of color in some overnight liquid cultures of certain high-copy-plasmid-borne CPs, and cautioning the use of multiple CPs as markers in competition assays. We solved these two problems by integrating pairs of these genes into the chromosome and by engineering versions of the same CP with very different colors.Conclusion: Availability of 14 engineered CP genes compared in E coil, together with chromosomal mutants suitable for competition assays, should simplify and expand CP study and applications. There was no single plasmid-borne CP that combined all of the most desirable features of intense color, fast maturation and low fitness cost, so this study should help direct future engineering efforts.
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- Boldemann, Cecilia, et al.
(författare)
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Impact of preschool environment upon children's physical activity and sun exposure.
- 2006
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Ingår i: Preventive medicine. - : Elsevier BV. - 0091-7435. ; 42:4, s. 301-8
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Tidskriftsartikel (refereegranskat)abstract
- The physical qualities of outdoor environments are important to trigger healthy behavior in children. We studied the impact of outdoor environments upon spontaneous physical activity and exposure to ultraviolet (UV) radiation in 4- to 6-year-old children at 11 preschools in Stockholm county.
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- Fregelius, Martin
(författare)
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An experimental approach on linear synthetic inertia
- 2021
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The interest in renewable energy has significantly increased in the last decades which has led to an increased amount of renewable energy sources in the grid. In the Nordic grid, the major contribution to renewable energy is hydro power and wind power and an increase in the amount of wind power is expected in the future. The increase in wind power and decommissioning of nuclear power is expected to decrease the mechanical inertia in the system which helps to stabilise the electrical grid frequency. The inertia is expected to decrease by a factor of two within 20 years and other solutions for frequency stability must be implemented to assure a stable power system. At Uppsala University several projects are investigating how grid-connected energy storages can increase the frequency stability with a high penetration of intermittent renewable energy sources. In this thesis, a linear synthetic inertia control algorithm is implemented on a national Instruments FPGA for controlling the power flow from a supercapacitor energy storage via a two-level three-phase inverter. The control strategy is evaluated both via simulations and experimental tests in a nano grid. The results of the simulations and experimental work are presented and show that it is possible to calculate the frequency derivative in real time to reduce the frequency ROCOF and nadir. The results of the increased frequency stability are presented.
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| 10. |
- Grabbe, Mårten, et al.
(författare)
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Design of an experimental setup for hydro-kinetic energy conversion
- 2009
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Ingår i: International Journal on Hydropower & Dams. - 1352-2523. ; 16:5, s. 112-116
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Tidskriftsartikel (refereegranskat)abstract
- A hydro-kinetic energy project has been underway in Sweden since 2000, and an in-stream prototype setup for experiments at a site in a Swedish river is now in progress. The system comprises a vertical axis turbine and a directly driven permanent magnet generator. Methods and choices used in designing the system are described here. The turbine and generator are evaluated based on measurements and CFD simulations of conditions at the site for the experimental setup.
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