| 1. |
- Aghabagherzadeh, Mozhdeh, et al.
(författare)
-
Folic Acid-Conjugated Chitosan-Coated Solid Lipid Nanoparticles: Precision Targeting of Artemisia vulgaris Essential Oils for Anticancer Therapy
- 2024
-
Ingår i: Chemistry and Biodiversity. - 1612-1880 .- 1612-1872. ; 21:1
-
Tidskriftsartikel (refereegranskat)abstract
- In this study, we developed Solid Lipid Nanoparticles (SLN-NPs) loaded with Artemisia vulgaris essential oil and coated with folic acid-chitosan (AVEO-SCF-NPs) to enhance drug delivery in biotechnology and pharmaceutical sectors. AVEO-SCF-NPs were synthesized using homogenization and ultra-sonication methods and comprehensively characterized. These nanoparticles exhibited a particle size of 253.67 nm, Polydispersity Index (PDI) of 0.26, zeta potential (zeta-p) of +39.96 mV, encapsulation efficiency (%EE) of 99.0 %, and folic acid binding efficiency (% FB) of 46.25 %. They effectively inhibited MCF-7, HT-29, and PC-3 cancer cells with IC50 values of 48.87 mu g/mL, 88.48 mu g/mL, and 121.34 mu g/mL, respectively, and demonstrated antibacterial properties against Gram-positive strains. AVEO-SCF-NPs also exhibited scavenging effects on ABTS (IC50: 203.83 mu g/mL) and DPPH (IC50: 680.86 mu g/mL) free radicals and inhibited angiogenesis, as confirmed through CAM and qPCR assays. Furthermore, these nanoparticles induced apoptosis, evidenced by up-regulation of caspase 3 and 9, down-regulation of TNF-alpha genes, and an increase in SubG1 phase cells. The high loading capacity of SCF-NPs for AVEO, coupled with their multifaceted biological properties, highlights AVEO-SCF-NPs as promising candidates for cancer therapy in the biotechnology and pharmaceutical industries.
|
|
| 2. |
- Bashlouei, Shima Ghareh, et al.
(författare)
-
Heracleum persicum Essential Oil Nanoemulsion: A Nanocarrier System for the Delivery of Promising Anticancer and Antioxidant Bioactive Agents
- 2022
-
Ingår i: Antioxidants. - : MDPI AG. - 2076-3921. ; 11:5
-
Tidskriftsartikel (refereegranskat)abstract
- Essential oils are important compounds for the prevention and/or treatment of various diseases in which solubility and bio-accessibility can be improved by nanoemulsion systems. Heracleum persicum oil nanoemulsion (HAE-NE) was prepared and biological properties were investigated against human breast cancer cells and normal human fibroblasts foreskin. Particle size, zeta potential and poly dispersity index were 153 nm, −47.9 mV and 0.35, respectively. (E)anethole (57.9%), terpinolene (13.8%), G-terpinene (8.1%), myrcene (6.8%), hexyl butyrate (5.2%), octyl bu-tanoate (4.5%) and octyl acetate (3.7%) was detected in nanoemulsion. Proliferation of cancer cells at IC50 = 2.32 µg/mL was significantly (p < 0.05) inhibited, and cell migration occurred at 1.5 µL/mL. The HAE-NE at 1.5, 2.5 and 3.5 µg/concentration up-regulated caspase 3 and enhanced sub-G1 peak of cell cycle with nil cytotoxic effects in the liver, kidney and jejunum of mice. Villus height, villus width, crypt depth and goblet cells in mice group fed with 10 and 20 mg/kg body weight of HAE-NE improved. Cellular redox state in the liver indicated 10 and 20 mg/kg body weight of nanoemulsion significantly up-regulated the expression of SOD, CAT and GPx genes. Heracleum persicum oil na-noemulsion could be an eco-friendly nanotherapeutic option for pharmaceutical, cosmetological and food applications.
|
|
| 3. |
- Bozorgi Kasgari, Mohammdamin, et al.
(författare)
-
Phytobiotic potential of Teucrium polium phenolic microcapsules against Salmonella enteritidis infection in mice
- 2023
-
Ingår i: Polymer Bulletin. - : Springer Science and Business Media LLC. - 0170-0839 .- 1436-2449. ; 80:2, s. 1681-1697
-
Tidskriftsartikel (refereegranskat)abstract
- Salmonella infection is a major public health issue worldwide. Given the alarming increase in infections with antibiotic-resistant microorganisms and side effects of synthetic antibiotics, there is an increasing interest in medicinal plants as natural alternatives to synthetic drugs to combat antibiotic-resistant microorganisms. The present study investigated the biological properties and health-promoting effects of microencapsulated phenolic compounds of Teucrium polium leaves as a dietary phytobiotic in mice challenged by Salmonella enteritidis. Microcapsules showed a capsulation efficiency of 93.6% with a particle size of 1261.8 nm with antioxidant and antibacterial activities in-vitro. The phytochemical analysis confirmed the presence of various phenolic compounds, i.e., ferulic acid, cinnamic acid, gallic acid, caffeic acid, salicylic acid, catechin, ellagic acid, and chrysin ranged between 121.6 and 975.3 µg/g DW. The microencapsulated phenolics exhibited improvement in the growth, liver function, morphometric parameters of ileum, antioxidant- and inflammation-related gene expression and inhibited the ileal population of S. enteritidis in mice challenged by S. enteritidis infection. The microencapsulated phenolics from T. polium leaves could be a promising phytobiotic approach to combat S. enteritidis infections.
|
|
| 4. |
- Liu, Jichao, et al.
(författare)
-
Betanin inhibits PI3K/AKT/mTOR/S6 signaling pathway, cell growth and death in osteosarcoma MG-63 cells
- 2023
-
Ingår i: Environmental Toxicology. - 1522-7278 .- 1520-4081. ; 38:9, s. 2173-2181
-
Tidskriftsartikel (refereegranskat)abstract
- It is possible to develop new chemopreventive compounds so that cancer cells can be targeted in an exclusive manner. Bioactive natural compounds have demonstrated to be efficient chemotherapeutic agents, safe and cost-effective. Majority of anti-cancer medications are derived from natural sources, particularly of plant origins. Betanin (betanidin-5-O-β-glucoside) is the most common betacyanin with antioxidant, anti inflammatory and anticancer properties. The present study therefore investigated the effect of betanin onosteosarcoma MG-63 cells. The mechanistic pathway of inflammatory responses, cell proliferation and apoptosis were investigated. The MG-63 cells were treated with betanin for 24 h. Betanin actions on the appearance of cell arrangements, morphological changes, ROS induced Δψm, cell migration, cell adhesion and proliferative mechanistic marker expression of PI3K/AKT/mTOR/S6were analyzed. Betanin inhibited MG-63 cells at IC50 concentrations between 9.08 and 54.49 μM and induced apoptosis by triggering the ROS mechanism. Betanin inhibited proliferation and migration of MG-63 cells and induced DNA fragmentation. Betanin also modified the key mediator expression levels of PI3K/AKT/mTOR/S6 signaling pathways. Betanin can potentially be utilized in bone carcinoma therapeutics to inhibit, reverse or delay osteosarcoma.
|
|
| 5. |
- Omidfar, Fatemeh, et al.
(författare)
-
Polyphenols in food industry, nano-based technology development and biological properties: An overview
- 2023
-
Ingår i: EFOOD. - 2666-3066. ; 4:3
-
Forskningsöversikt (refereegranskat)abstract
- There is a growing interest in bioactive compounds particularly polyphenols as dietary sources, food ingredients with several putative beneficial activities such as antioxidant and antimicrobial properties. Understanding the underlying mechanisms of plant-derived phenolic compounds can substantially help develop solutions and technologies to better control and alleviate oxidative stress, inflammation, and consequently cancer. Studies have indicated that the loading of natural phytoconstituents into appropriate nanoparticles can develop and broaden biological applications. There is a wide range of applications derived from nanotechnology and nanomedicine through developing the packaging, preservation, and bioavailability of natural phytochemical. The present paper provides an overview and highlight on the importance and potential of natural polyphenols particularly in food and pharmaceutical industries. Furthermore, nanotechnology approaches, bioavailability, and potential adverse effects are also discussed. This present review highlighted the importance and potential of natural polyphenols which is widely used in food and pharmaceutical industries. Besides that, the nanotechnology approaches, bioavailability, and their potential adverse impact are also discussed.image
|
|
| 6. |
- Pei, Jinjin, et al.
(författare)
-
Effects of Syringic Acid on Apoptosis, Inflammation, and AKT/mTOR Signaling Pathway in Gastric Cancer Cells
- 2021
-
Ingår i: Frontiers in Nutrition. - : Frontiers Media SA. - 2296-861X. ; 8
-
Tidskriftsartikel (refereegranskat)abstract
- Gastric cancer is one of the most common cancer and deadly disease worldwide. Despite substantial advances made in the treatment of gastric cancer, existing therapies still encounter bottlenecks. Chemotherapy, for instance, could lead to serious side effects, high drug resistance and treatment failure. Phytochemical-derived compounds from plants offer novel strategies as potent drug molecules in cancer therapy. Given the low toxicity and higher tolerance rate of naturally occurring compounds, the present study evaluated the effects of syringic acid on cytotoxicity, oxidative stress, mitochondrial membrane potential, apoptosis, and inflammatory responses in gastric cancer cell line (AGS). AGS cells were treated with various concentrations (5–40 μg/mL) of syringic acid for 24 h, after which cytotoxicity was analyzed. Reactive Oxygen Species (ROS), antioxidant enzyme activities, mitochondrial membrane potential (MMP, Δψm), cell morphologies, the expression of apoptotic markers and protein expression patterns were also investigated. Results indicated that syringic acid-treated cells developed anti-cancer activities by losing MMP, cell viability, and enhancing intracellular ROS. Syringic acid selectively developed apoptosis in a dose-dependent manner via enhanced regulation of caspase-3, caspase-9 and Poly ADP-ribose Polymerase (PARP) whereas decreasing the expression levels of p53 and BCL-2. Syringic acid also lowered activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) whereas Thio Barbituric Acid Reactive Substances (TBARS) increased. Syringic acid suppressed gastric cancer cell proliferation, inflammation, and induced apoptosis by upregulating mTOR via AKT signaling pathway. The study suggests syringic acid may constitute a promising chemotherapeutic candidate for gastric cancer treatment. Our study is the first report on the anti-cancer effects of syringic acid against gastric cancer cells via apoptosis, inhibition of inflammation, and the suppression of the mTOR/AKT signaling pathway.
|
|
| 7. |
- Pilban Jahromi, Siamak, et al.
(författare)
-
The effect of supporting electrolyte concentrations on the growth of nickel nanostructures
- 2021
-
Ingår i: Journal of Alloys and Compounds. - : Elsevier BV. - 0925-8388. ; 880
-
Tidskriftsartikel (refereegranskat)abstract
- Nickel nanostructures (Ni NSs) were grown on poly N-methyl pyrrole (PMPy) surface via facile electrochemical deposition method. Morphology and crystalline properties of the fabricated Ni NSs were characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), and cyclic voltammetry (CV). The size-strain plot model was employed to determine the average crystallite sizes of Ni NSs prepared at 0.001, 0.01 and 0.1 M of KCl concentrations, respectively. Results indicated that Ni NSs morphologies were influenced by the concentration of KCl electrolyte in which utilized as supporting electrolyte. The average crystallite size decreased as the KCl electrolyte concentration increased. An irregular structure consisting of microspheres with an average size of 2 µm, nanodendritic structure with an average thickness of 120 nm and a large-scale uniform nanowall structure with an average thickness of 70 nm were formed in the presence of 0.001, 0.01 and 0.1 M KCl concentrations, respectively. The electrochemical behavior of Ni NSs characterized by cyclic voltammetry (CV) in 1 M solution of KOH revealed an improvement in the redox reaction of the Ni nano wall (Ni NW) compared to other Ni NSs electrodes, thus making Ni NW a good candidate for future utilization in electrode materials of fuel cells.
|
|
| 8. |
- Rad, Negar Chalaki, et al.
(författare)
-
Assessment of nanoliposomes loaded with daidzein for ameliorating diabetes in alloxan-induced mice: A promising nutraceutical approach
- 2023
-
Ingår i: Journal of Functional Foods. - 1756-4646. ; 110
-
Tidskriftsartikel (refereegranskat)abstract
- The present study synthesized and evaluated the effects of nanoliposomes loaded daidzein (NLD) in diabetic mice induced by alloxan. Five groups of Balb-c mice were treated for 5 weeks (Group 1 and 2 referred to as normal and diabetic control receiving normal feed). Group 3 and 4 were diabetic mice receiving 50 and 100 mg/kg/BW NLD respectively. Group 5, diabetic mice, received the standard drug, Metformin, 250 mg/kg/BW per day orally. Results indicated that NLD significantly (p < 0.05) improved the final body weight, triglyceride (TG), total cholesterol (TC) concentrations, low-density lipoprotein cholesterol and malondialdehyde (MDA) levels and enhanced the high-density lipoprotein. Furthermore, NLD enhanced the expression of CAT and suppressed iNOS gene in the pancreas. The mRNA expression of GLUT2 and GLUT4 also showed increase in the diabetic mice. Findings demonstrated the potential of NLD as a nutraceutical for the prevention and/or treatment of diabetes without toxic side effects.
|
|
| 9. |
- Rahimi, Shadi, 1982, et al.
(författare)
-
Cellular and subcellular interactions of graphene-based materials with cancerous and non-cancerous cells
- 2022
-
Ingår i: Advanced Drug Delivery Reviews. - : Elsevier BV. - 0169-409X .- 1872-8294. ; 189
-
Forskningsöversikt (refereegranskat)abstract
- Despite significant advances in early detection and personalized treatment, cancer is still among the leading causes of death globally. One of the possible anticancer approaches that is presently receiving a lot of attention is the development of nanocarriers capable of specific and efficient delivery of anticancer drugs. Graphene-based materials are promising nanocarriers in this respect, due to their high drug loading capacity and biocompatibility. In this review, we present an overview on the interactions of graphene-based materials with normal mammalian cells at the molecular level as well as cellular and subcellular levels, including plasma membrane, cytoskeleton, and membrane-bound organelles such as lysosomes, mitochondria, nucleus, endoplasmic reticulum, and peroxisome. In parallel, we assemble the knowledge about the interactions of graphene-based materials with cancerous cells, that are considered as the potential applications of these materials for cancer therapy including metastasis treatment, targeted drug delivery, and differentiation to non-cancer stem cells. We highlight the influence of key parameters, such as the size and surface chemistry of graphene-based materials that govern the efficiency of internalization and biocompatibility of these particles in vitro and in vivo. Finally, this review aims to correlate the key parameters of graphene-based nanomaterials specially graphene oxide, such as size and surface modifications, to their interactions with the cancerous and non-cancerous cells for designing and engineering them for bio-applications and especially for therapeutic purposes.
|
|
| 10. |
- Rahmani, Fatemeh, et al.
(författare)
-
Thyme Oil Nanoemulsion Enhanced Cellular Antioxidant and Suppressed Inflammation in Mice Challenged by Cadmium-Induced Oxidative Stress
- 2022
-
Ingår i: Waste and Biomass Valorization. - : Springer Science and Business Media LLC. - 1877-2641 .- 1877-265X. ; 13:7, s. 3139-3146
-
Tidskriftsartikel (refereegranskat)abstract
- Cadmium is a poisonous trace element which induces oxidative stress and pollutes the environment. Nanoemulsions are recognized as a new drug delivery system with enhanced therapeutic efficacy. In the present study, nanoemulsion of Thymus vulgaris essential oil was prepared and characterized. The effects of the essential oil on body weight gain, liver mineral content, histopathology, lipid peroxidation, antioxidant- and inflammatory-related gene expressions were also investigated in mice challenged by cadmium-induced oxidative stress. Characterization of nanoemulsion (e.g., polydispersity index, particle size, and ζ-potential) indicated a good stability degree of T. vulgaris essential oil. Phytochemical analysis by GC–MS also demonstrated T. vulgaris essential oil contained phenolic compounds i.e., thymol, p-cymene, ɣ-teripinene, carvacrol, caryophyllene and linalool. The treatment of mice with T. vulgaris essential oil significantly (p < 0.05) improved body weight changes, reduced cadmium deposition in the liver and decreased lipid peroxidation compared to control group. Also, the antioxidative potential was enhanced whereas inflammation in the tissues were suppressed. The GPx gene was up regulated whereas iNOS genes were significantly (p < 0.05) downregulated in kidney, liver and brain tissues. Our findings suggest T.vulgaris essential oil can be a promising protective agent against cadmium-induced oxidative stress.
|
|
