| 1. |
- Zhang, Xin, et al.
(författare)
-
Light-Up Lipid Droplets Dynamic Behaviors Using a Red-Emitting Fluorogenic Probe
- 2020
-
Ingår i: Analytical Chemistry. - : AMER CHEMICAL SOC. - 0003-2700 .- 1520-6882. ; 92:5, s. 3613-3619
-
Tidskriftsartikel (refereegranskat)abstract
- Intracellular lipid metabolism occurs in lipid droplets (LDs), which is critical to the survival of cells. Imaging LDs is an intuitive way to understand their physiology in live cells. However, this is limited by the availability of specific probes that can properly visualize LDs in vivo. Here, an LDs-specific red-emitting probe is proposed to address this need, which is not merely with an ultrahigh signal-to-noise (S/N) ratio and a large Stokes shift (up to 214 nm) but also with superior resistance to photobleaching. The probe has been successfully applied to real-time tracking of intracellular LDs behaviors, including fusion, migration, and lipophagy processes. We deem that the proposed probe here offers a new possibility for deeper understanding of LDs-associated behaviors, elucidation of their roles and mechanisms in cellular metabolism, and determination of the transition between adaptive lipid storage and lipotoxicity as well.
|
|
| 2. |
- Wang, Hong, et al.
(författare)
-
Real-time tracking of mitochondrial dynamics by a dual-sensitive probe
- 2020
-
Ingår i: Sensors and actuators. B, Chemical. - : Elsevier. - 0925-4005 .- 1873-3077. ; 320
-
Tidskriftsartikel (refereegranskat)abstract
- Tracking of mitochondrial dynamic processes including their morphology, biogenesis and elimination is of significance to deeply understand mitochondrial physiology and related diseases, which requires simultaneous and discriminative labeling of both mitochondria themselves and correlated downstream organelles. However, the combination of multiple fluorescent probes will bring the large invasive effect that seriously impacts the normal physiological processes of mitochondria within live cells. To address this issue, we herein construct a single molecular probe through the combination of two pH-sensitive fluorogens, hydroxyl-coumarin and rhodamine B, enabling the detection of intracellular microenvironmental pH variations. Importantly, the as-synthesized probe not only accumulates into mitochondria, indicated by cyan emission from hydroxyl-coumarin but also can be delivered to the downstream acidic autolysosomes upon the occurrence of mitophagy, indicated by red emission from rhodamine B. Taking advantage of a two-channel confocal microscopy strategy, we have achieved to real-time track the processes of mitochondrial replication, merge and autophagy.
|
|
| 3. |
- zhang, xin, 1990-, et al.
(författare)
-
Single-wavelength-excited fluorogenic nanoprobe for accurate real-time ratiometric analysis of broad pH fluctuations in mitophagy
- 2022
-
Ingår i: Nano Reseach. - : Tsinghua University Press. - 1998-0124 .- 1998-0000. ; 15, s. 6515-6521
-
Tidskriftsartikel (refereegranskat)abstract
- Mitophagy has a critical role in maintaining cellular homeostasis through acidic lysosomes engulfing excess or impaired mitochondria, thereby pH fluctuation is one of the most significant indicators for tracking mitophagy. Then such precise pH tracking demands the fluorogenic probe that has tailored contemporaneous features, including mitochondrial-specificity, excellent biocompatibility, wide pH-sensitive range of 8.0-4.0, and especially quantitative ability. However, available molecular probes cannot simultaneously meet all the requirements since it is extremely difficult to integrate multiple functionalities into a single molecule. To fully address this issue, we herein integrate two fluorogenic pH sensitive units, a mitochondria-specific block, cell-penetrating facilitator, and biocompatible segments into an elegant silica nano scaffold, which greatly ensures the applicability for real-time tracking of pH fluctuations in mitophagy. Most significantly, at a single wavelength excitation, the integrated pH-sensitive units have spectra-distinguishable fluorescence towards alkaline and acidic pH in a broad range that covers mitochondrial and lysosomal pH, thus enabling a ratiometric analysis of pH variations during the whole mitophagy. This work also provides constructive insights into the fabrication of advanced fluorescent nanoprobes for diverse biomedical applications.
|
|
| 4. |
- Hu, Zhang-Jun, et al.
(författare)
-
Real-time visualizing the regulation of reactive oxygen species on Zn2+ release in cellular lysosome by a specific fluorescent probe
- 2018
-
Ingår i: Sensors and actuators. B, Chemical. - : ELSEVIER SCIENCE SA. - 0925-4005 .- 1873-3077. ; 264, s. 419-425
-
Tidskriftsartikel (refereegranskat)abstract
- Reactive oxygen species (ROS) regulating the release of free zinc ions (Zn2+) in cellular lysosome is closely related to various pathways of cellular signal transduction, such as inflammation and oxidative stress. Directly visualizing Zn2+ release in lysosome is essential for in-depth understanding these physiological processes, and is still an atelic challenge. In this work, we successfully fabricate a lysosome-specific Zn2+ fluorescent probe and achieve the visualization of ROS-induced Zn2+ release in lysosome of inflammatory cells. The as-prepared probe combines a green fluorophore, an ionophore with five-dentate sites, and a morpholine as the lysosome-specific localization moiety. The fluorescence of the fluorophore in the free probe is suppressed by a photoinduced electron transfer (PET) process from nitrogen atoms in the ionophore. Upon the addition of Zn2+, the fluorescence can be promoted immediately, achieving the real-time detection. Meanwhile, the probe is sensitive and selective to Zn2+, which provides the capability to detect low-concentration of free Zn2+ in lysosomes. Accordingly, the Zn2+ release was clearly observed in lysosome with the increase of ROS levels when the inflammation occurred in living cells. (c) 2018 Published by Elsevier B.V.
|
|
| 5. |
- Ou, Pan, et al.
(författare)
-
Gasotransmitter Regulation of Phosphatase Activity in Live Cells Studied by Three-Channel Imaging Correlation
- 2019
-
Ingår i: Angewandte Chemie International Edition. - : WILEY-V C H VERLAG GMBH. - 1433-7851 .- 1521-3773. ; 58:8, s. 2261-2265
-
Tidskriftsartikel (refereegranskat)abstract
- Enzyme activity in live cells is dynamically regulated by small-molecule transmitters for maintaining normal physiological functions. A few probes have been devised to measure intracellular enzyme activities by fluorescent imaging, but the study of the regulation of enzyme activity via gasotransmitters in situ remains a long-standing challenge. Herein, we report a three-channel imaging correlation by a single dual-reactive fluorescent probe to measure the dependence of phosphatase activity on the H2S level in cells. The two sites of the probe reactive to H2S and phosphatase individually produce blue and green fluorescent responses, respectively, and resonance energy transfer can be triggered by their coexistence. Fluorescent analysis based on the three-channel imaging correlation shows that cells have an ideal level of H2S to promote phosphatase activity up to its maximum. Significantly, a slight deviation from this H2S level leads to a sharp decrease of phosphatase activity. The discovery further strengthens our understanding of the importance of H2S in cellular signaling and in various human diseases.
|
|
| 6. |
- Shen, Jie, et al.
(författare)
-
Real-time monitoring of lipid droplets growth via the fusion with fluorescent dye-labeled adiposomes
- 2020
-
Ingår i: Dyes and pigments. - : ELSEVIER SCI LTD. - 0143-7208 .- 1873-3743. ; 182
-
Tidskriftsartikel (refereegranskat)abstract
- Investigating lipid droplets (LDs) behaviours is essential to deeply understand the physiology of LDs, such as their growths, movements, fusion/division, and autophagy. Among these behaviours, the growth of LDs is one of the most difficult to track due to the very subtle morphology evolution in a short time window. The major obstacle is that conventional LDs-specific dyes with low photostability cannot indicate the LDs size change. To address this issue, we synthesize a hydrophobic and photostable fluorescent dye (TPA-AD) and load it into the neutral lipid micelles (as artificial adiposomes). The highly hydrophobic TPA-AD enables the specific accumulation into intracellular LDs and the ready loading artificial adiposomes. When the intracellular LDs take TPA-AD-labeled adiposomes, by fusion, the sizes of LDs gradually grow, and LDs are simultaneously lighted up by the fluorescence of TPA-AD. Importantly, the high photostability of TPA-AD ensures the enhanced fluorescence signals. The finding here will further strengthen the understanding of LDs dynamics and fat metabolism.
|
|
| 7. |
- Yang, Guanqing, et al.
(författare)
-
A Multi-responsive Fluorescent Probe Reveals Mitochondrial Nucleoprotein Dynamics with Reactive Oxygen Species Regulation through Super-resolution Imaging
- 2020
-
Ingår i: Angewandte Chemie International Edition. - : WILEY-V C H VERLAG GMBH. - 1433-7851 .- 1521-3773. ; 59:37, s. 16154-16160
-
Tidskriftsartikel (refereegranskat)abstract
- Understanding the biomolecular interactions in a specific organelle has been a long-standing challenge because it requires super-resolution imaging to resolve the spatial locations and dynamic interactions of multiple biomacromolecules. Two key difficulties are the scarcity of suitable probes for super-resolution nanoscopy and the complications that arise from the use of multiple probes. Herein, we report a quinolinium derivative probe that is selectively enriched in mitochondria and switches on in three different fluorescence modes in response to hydrogen peroxide (H2O2), proteins, and nucleic acids, enabling the visualization of mitochondrial nucleoprotein dynamics. STED nanoscopy reveals that the proteins localize at mitochondrial cristae and largely fuse with nucleic acids to form nucleoproteins, whereas increasing H(2)O(2)level leads to disassociation of nucleic acid-protein complexes.
|
|
| 8. |
- Gong, Zheng, et al.
(författare)
-
A targetable fluorescent probe for detecting mitochondrial viscosity in live cells by using fluorescence lifetime imaging
- 2024
-
Ingår i: New Journal of Chemistry. - : ROYAL SOC CHEMISTRY. - 1144-0546 .- 1369-9261.
-
Tidskriftsartikel (refereegranskat)abstract
- Intracellular viscosity plays an important role in regulating cellular morphology and physiology and is closely related to a host of diseases. Especially, the changes in mitochondrial viscosity will cause some common diseases such as hyperlipidemia, Alzheimer's disease and cancer. In this work, we report the design of a red-emissive molecular rotor for the detection of mitochondrial viscosity in live cells. The probe showed fascinating performance, such as specific targeting to mitochondria, high sensitivity to viscosity, and rapid fluorescence response, especially the dual response mode of fluorescence intensity and fluorescence lifetime. By using this probe, we realized monitoring of the mitochondrial viscosity variations in live cells under different physiological processes. Our study offers an opportunity to discover potential tools for mitochondria-related physiology and pathology investigation. A D-pi-A typed fluorescence lifetime probe for sensitively detecting viscosity has been designed and synthesized. BSOH has been successfully applied to real-time monitoring mitochondrial viscosity in live cells by fluorescence lifetime imaging.
|
|
| 9. |
- Zhang, Kai, et al.
(författare)
-
Synthesis of a Neo-Confused Octaphyrin and the Formation of Its Mononuclear Complexes
- 2015
-
Ingår i: Organic Letters. - : American Chemical Society (ACS). - 1523-7060 .- 1523-7052. ; 17:19, s. 4806-4809
-
Tidskriftsartikel (refereegranskat)abstract
- Novel neo-confused octaphyrin(1.1.1.1.1.1.1.0) (1) was synthesized by oxidative ring closure of an octapyrrane bearing two terminal "confused" pyrroles. Crystal structures of its Zn(II) and Cu(II) complexes (2 and 3) show a figure-of-eight conformation with unique mononuclear coordination structures. Photophysical data and theoretical calculations suggest that the neo-confused octaphyrin 1 is a 34 pi electron conjugated species showing nonaromaticity. Coordination of pocopper and zinc ions results in the further narrowing of the HOMO-LUMO gaps.
|
|
| 10. |
- Li, Cong, et al.
(författare)
-
Expression of Twist associated to microcirculation patterns of human glioma correlated with progression and survival of the patient
- 2020
-
Ingår i: NOVEL THERAPEUTIC ADVANCES IN GLIOBLASTOMA. - LONDON ENGLAND : Elsevier. - 9780128211144 ; , s. 201-217
-
Bokkapitel (refereegranskat)abstract
- Twist is a transcription factor involved in the process of epithelial to mesenchymal transition (EMT) of carcinoma cells, and the promotion of invasion of gliomas through the mesenchymal adjusting process. However, its clinical significance in human glioma has not yet to be understood. To delineate the clinical-pathological significance and prognostic value of Twist, the expression of Twist was evaluated by Immunohistochemistry for 187 glioma samples. We found that Twist demonstrated frequent nuclear expression in the glioma samples and its expression levels were associated with tumor grade (P < 0.001). Furthermore, high Twist expression was correlated with a poor outcome in patients with glioma (P = 0.001), particularly with high grade glioma (P = 0.026). Interestingly, Twist expression showed positive correlation with microvascular density (MVD) (r = 0.145, P = 0.048) as well as vasculogenic mimicry (VM) (r = 0.273, P < 0.001) in the tumors. These results suggest that Twist could be a predictor for poor prognosis in glioma patients. Additionally, Twist expression was associated with two major microcirculation patterns: endothelial-dependent vessels and VM in glioma, indicating that Twist could be a potential molecular target for anti-glioma therapy.
|
|
