| 1. |
- Zhao, Peng, et al.
(författare)
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Lentiviral vector mediated siRNA knock-down of hTERT results in diminished capacity in invasiveness and in vivo growth of human glioma cells in a telomere length-independent manner
- 2007
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Ingår i: International Journal of Oncology. - 1019-6439. ; 31:2, s. 361-368
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Tidskriftsartikel (refereegranskat)abstract
- Glioma cells are characterized by their invasiveness and resistance against conventional therapeutics. Telomerase activity has been suggested to be an important target for glioma treatment. Here we assessed the anticancer effects and its potential mechanisms of lentiviral vector mediated siRNA knock-down of the human telomerase reverse transcriptase (hTERT) in U87MG human glioblastoma cells. Stable expression of anti-hTERT siRNA reduced the hTERT expression and TRAP assay telomerase activity to barely detectable levels. Injection of lentiviral vectors encoding anti-hTERT siRNA significantly inhibited the growth of preestablished macroscopic xenograft tumors, which was in contrast to the finding that no obvious effects on cell growth, cell cycle progression and telomere length were observed in anti-hTERT siRNA expressing U87MG cells during short-term in vitro cultures. The in vivo glioma growth inhibition effect was already evident in the period coincided with no detectable telomere length changes, suggesting that hTERT inhibition may hinder glioma cell growth in a telomere length-independent manner. Importantly, transwell migration assay showed profound inhibitory effect on the invasive capacity of U87MG cells following short-term anti-hTERT siRNA expression. Thus, efficient knock-down of hTERT can inhibit glioma cell proliferation and migration prior to its effect on telomere length.
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| 2. |
- Zhao, Peiyu, et al.
(författare)
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Stable lithium metal anode enabled by high-dimensional lithium deposition through a functional organic substrate
- 2020
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Ingår i: Energy Storage Materials. - : Elsevier BV. - 2405-8297. ; 33, s. 158-163
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Tidskriftsartikel (refereegranskat)abstract
- The growth of lithium dendrites severely restricts the development of lithium metal batteries. In order to achieve the goal of dendrites-free lithium in principle, it is crucial and urgent to control nucleation and growth of lithium. Here, a functional organic layer of perylene-3, 4, 9, 10-tetracarboxydiimide-lithium (PTCDI-Li) is built on the lithium anode surface by in-situ chemical reaction of PTCDI and Li metal. PTCDI-Li, with high surface energy (-10.19 eV) and low diffusion barrier (0.89 eV), efficiently promotes disk-shaped high-dimensional nucleation by regulation of lithium ion flux upon lithium plating, leading to a dendrites-free morphology. When operating under a relatively high current density of 10 mA cm−2, the Li | Li symmetrical cells with PTCDI-Li exhibit outstanding cyclic stability for 300 hours with ultralow overpotential of 400 mV, superior to the most of the reported lithium anode. The corresponding PTCDI-Li batteries show high specific capacity and enhanced cycle life. We anticipate that this strategy of regulation of lithium deposition from one-dimensional to high-dimensional opens a new horizon in the development of dendrites-free Li anodes.
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