| 1. |
- Adlerberth, Josefin, et al.
(författare)
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Thermometric analysis of blood metabolites in ICU patients
- 2020
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Ingår i: Journal of Thermal Analysis and Calorimetry. - : Springer Science and Business Media LLC. - 1388-6150 .- 1588-2926. ; 140:2, s. 763-771
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Tidskriftsartikel (refereegranskat)abstract
- Real-time monitoring of patient’s blood metabolites, such as glucose and lactate, could potentially improve surgery and recovery outcomes for patients in surgical and intensive care units. Our enzyme thermometric biosensor which is based on flow injected calorimetric determination of immobilized enzyme reaction is capable of performing continuous, fast, and quantitative analysis of metabolites using whole blood. A key technical advantage the assay affords is the ability to use unpretreated whole blood. In this article, the enzyme thermometric biosensor was used, for the first time, to determine glucose and lactate concentrations in the blood of ICU patients. The linear detection range for glucose was 0.5–30 mM and 0.25–12 mM for lactate, using a 20 μL sample volume. A maximum sampling rate of 15 measurements per hour was achieved using venous blood samples, which corresponds to a 4-min measurement interval. In order to validate the accuracy of the results, a comparative analysis between the thermometric biosensor and the clinically applied instrument (LifeScan’s OneTouch®) which is based on disposable dry chemical reaction was performed using samples from 33 patients. The results showed a good correlation between the two methods for both glucose (r = 0.843, p < 0.0001) and lactate (r = 0.78, p = 0.0105). The ability to monitor metabolite levels and trends on a clinically relevant timescale of 5 min is critical for intensive monitoring of ICP and operative patients.
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| 2. |
- Chen, Ying, et al.
(författare)
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Dual-signal analysis eliminates requirement for milk sample pretreatment.
- 2011
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Ingår i: Biosensors & Bioelectronics. - : Elsevier BV. - 1873-4235 .- 0956-5663. ; 29:1, s. 115-118
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Tidskriftsartikel (refereegranskat)abstract
- Detection of analytes in complex biological samples, such as milk and blood, normally requires sample pretreatment. These pretreatment regimes reduce assay throughput and increase testing costs. Technologies that make it possible to eliminate sample pretreatment are of great industrial interest. Here we report the development of a dual-signal flow injected analysis device which eliminates the need for sample pretreatment. The device employs thermal traducers to measure the signal from an enzyme and a reference column. This makes it possible to independently monitor and correct for non-specifically generated heat, thereby eliminating the need for sample pretreatment. The ability of the dual-signal device to determine urea and lactate in milk samples without any prior treatment was evaluated. The spiked milk samples, the urea assay had a linear range from 0.1 to 50mM (R=0.996), and the lactate assay had a linear range from 0.025 to 5.0mM (R=0.9998). The linear regression values for urea and lactate for 0.5%, 1.5% and 3.0% fat milk were at least 0.990. The dual-signal design improves assay reproducibility, accuracy and sensitivity. Addition benefits are shorter assay times and lowers costs, as well as reducing equipment and training requirements. The potential application of the technology for multi-analyte analysis in point of care and decentralized diagnostic testing in healthcare, agriculture and environmental areas is discussed.
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| 3. |
- Li, Qiang, et al.
(författare)
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Asymmetric plasmonic-dielectric coupler with short coupling length, high extinction ratio, and low insertion loss
- 2010
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Ingår i: Optics Letters. - 0146-9592 .- 1539-4794. ; 35:19, s. 3153-3155
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Tidskriftsartikel (refereegranskat)abstract
- Asymmetric directional coupling between a hybrid plasmonic waveguide with subwavelength field confinement and a conventional dielectric waveguide is investigated. The proposed hybrid coupler features short coupling length, high coupling efficiency, high extinction ratio, and low insertion loss; it can also be integrated into a silicon-based platform. This coupler can be potentially adopted for signal routing between plasmonic waveguides and dielectric waveguides in photonic integrated circuits. Furthermore, it can be exploited to efficiently excite hybrid plasmonic modes with conventional dielectric modes.
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| 4. |
- Mecklenburg, Michael, et al.
(författare)
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A Microarray platform for "omics" analysis
- 2001
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Ingår i: International Conference on Sensor Technology (ISTC 2001). - : SPIE. - 0277-786X. - 0819441198 - 9780819441195 ; 4414, s. 157-163
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Konferensbidrag (refereegranskat)abstract
- Microarray technology has revolutionized genetic analysis. However, limitations in genome analysis has lead to renewed interest in establishing 'omic' strategies. As we enter the post-genomic era, new microarray technologies are needed to address these new classes of 'omic' targets, such as proteins, as well as lipids and carbohydrates. We have developed a microarray platform that combines self- assembling monolayers with the biotin-streptavidin system to provide a robust, versatile immobilization scheme. A hydrophobic film is patterned on the surface creating an array of tension wells that eliminates evaporation effects thereby reducing the shear stress to which biomolecules are exposed to during immobilization. The streptavidin linker layer makes it possible to adapt and/or develop microarray based assays using virtually any class of biomolecules including: carbohydrates, peptides, antibodies, receptors, as well as them ore traditional DNA based arrays. Our microarray technology is designed to furnish seamless compatibility across the various 'omic' platforms by providing a common blueprint for fabricating and analyzing arrays. The prototype microarray uses a microscope slide footprint patterned with 2 by 96 flat wells. Data on the microarray platform will be presented.
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| 5. |
- Yao, Na, et al.
(författare)
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A Novel Thermal Sensor for the Sensitive Measurement of Chemical Oxygen Demand.
- 2015
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Ingår i: Sensors. - : MDPI AG. - 1424-8220. ; 15:8, s. 20501-20510
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Tidskriftsartikel (refereegranskat)abstract
- A novel rapid methodology for determining the chemical oxygen demand (COD) based on a thermal sensor with a flow injection analysis system was proposed and experimentally validated. The ability of this sensor to detect and monitor COD was based on the degree of enthalpy increase when sodium hypochlorite reacted with the organic content in water samples. The measurement results were correlated with COD and were compared against the conventional method using potassium dichromate. The assay required only 5-7 min rather than the 2 h required for evaluation by potassium dichromate. The linear range was 5-1000 mg/L COD, and the limit of detection was very low, 0.74 mg/L COD. Moreover, this method exhibited high tolerance to chloride ions; 0.015 mol/L chloride ions had no influence on the response. Finally, the sensor was used to detect the COD of different water samples; the results were verified by the standard dichromate method.
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