| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Li, Furong, et al.
(författare)
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Relative pollen productivity estimates for major plant taxa of cultural landscapes in central eastern China
- 2017
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Ingår i: Vegetation History and Archaeobotany. - : Springer. - 0939-6314 .- 1617-6278. ; 26:6, s. 587-605
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Tidskriftsartikel (refereegranskat)abstract
- In this study we estimate relative pollen productivity (RPP) for plant taxa characteristic of human-induced vegetation in ancient cultural landscapes of the low mountain ranges of Shandong province in eastern temperate China. RPP estimates are required to achieve pollen-based reconstructions of Holocene plant cover using modelling approaches based on Prentice's and Sugita's theoretical background and models (REVEALS and LOVE). Pollen counts in moss samples and vegetation data from 36 sites were used in the Extended R-Value (ERV) model to estimate the relevant source area of pollen (RSAP) of moss polsters and RPP of major plant taxa. The best results were obtained with the ERV sub-model 3 and Prentice's taxon-specific method (using a Gaussian Plume dispersal model) to distance weight vegetation data. RSAP was estimated to 145 m using the maximum likelihood method. RPP was obtained for 18 taxa of which two taxa had unreliable RPP (Amaranthaceae/Chenopodiaceae and Vitex negundo). RPPs for Castanea, Cupressaceae, Robinia/Sophora, Aster/Anthemis-type, Cannabis/Humulus, Caryophyllaceae, Brassicaceae and Galium-type are the first ones for China. Trees, except Robinia/Sophora (RPP = 0.78 +/- 0.03) have larger RPPs than herbs other than Artemisia (RPP = 24.7 +/- 0.36). The RPPs for Quercus, Pinus and Artemisia are comparable with other RPPs obtained in China, the RPPs for Pinus, Quercus, Ulmus, Cyperaceae and Galium-type with the mean RPPs obtained in Europe, and RPP for Cupressaceae with that for Juniperus in Europe. The values for Aster/Anthemis-type, Caryophyllaceae, Asteraceae SF Cichorioideae and Juglans differ from the few RPPs available in China and/or Europe.
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| 3. |
- Li, Xuping, et al.
(författare)
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A three-dimensional ratiometric sensing strategy on unimolecular fluorescence-thermally activated delayed fluorescence dual emission
- 2019
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Ingår i: Nature Communications. - : NATURE PUBLISHING GROUP. - 2041-1723. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- Visualized sensing through fluorescence signals is a powerful method for chemical and physical detection. However, the utilization of fluorescent molecular probes still suffers from lack of precise signal self-calibration in practical use. Here we show that fluorescence and thermally activated delayed fluorescence can be simultaneously produced at the single-molecular level. The thermally activated delayed fluorescence serves as a sensing signal with its wavelength and lifetime both altered correlating to polarity, whereas the fluorescence always remains unchanged as an internal reference. Upon the establishment of a three-dimensional working curve upon the ratiometric wavelength and photoluminescence lifetime vs. polarity, disturbance factors during a relevant sensing process can be largely minimized by such a multiple self-calibration. This strategy was further applied into a precise detection of the microenvironmental polarity variation in complex phospholipid systems, towards providing new insights for convenient and accurate diagnosis of membrane lesions.
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| 4. |
- Zhao, Chunrui, et al.
(författare)
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Large red-shifted NIR absorption in azulenyl- and iodinated-modified BODIPYs sensitive to aggregation and protonation stimuli
- 2022
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Ingår i: Dyes and pigments. - : Elsevier. - 0143-7208 .- 1873-3743. ; 197
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Tidskriftsartikel (refereegranskat)abstract
- Pushing forward the red-shifting of electronic transitions in organic dyes is potentially important for applications in photodynamical chemistry and therapies. In this work, a series of BODIPY derivatives conjugated with azulene at different substituent positions were synthesized and investigated in depth. These compounds are shown to exhibit varying bathochromic shifts of their electronic transitions, as well as a further red-shift of the absorption by the modification of the compounds with iodine. Moreover, self-assembly and protonation of these compounds lead to hundreds of nanometer red-shifted electronic transitions, up to NIR-II window, relative to traditional BODIPYs. On this basis, a unique photoproduction of singlet oxygen species can be achieved under corresponding long-wavelength irradiation both in organic solution and in water, featuring a superior photosensitivity and photodynamic potential of the molecular design.
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| 5. |
- Zheng, Fenglian, et al.
(författare)
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Structural and functional hippocampal alterations in Multiple sclerosis and neuromyelitis optica spectrum disorder
- 2022
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Ingår i: Multiple Sclerosis Journal. - : SAGE Publications. - 1352-4585 .- 1477-0970. ; 28:5, s. 707-717
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Tidskriftsartikel (refereegranskat)abstract
- Background: Hippocampal involvement may differ between multiple sclerosis (MS) and neuromyelitis optica spectrum disorder (NMOSD). Objective: To investigate the morphometric, diffusion and functional alterations in hippocampus in MS and NMOSD and the clinical significance. Methods: A total of 752 participants including 236 MS, 236 NMOSD and 280 healthy controls (HC) were included in this retrospective multi-center study. The hippocampus and subfield volumes, fractional anisotropy (FA) and mean diffusivity (MD), amplitude of low frequency fluctuation (ALFF) and degree centrality (DC) were analyzed, and their associations with clinical variables were investigated. Results: The hippocampus showed significantly lower volume, FA and greater MD in MS compared to NMOSD and HC (p < 0.05), while no abnormal ALFF or DC was identified in any group. Hippocampal subfields were affected in both diseases, though subiculum, presubiculum and fimbria showed significantly lower volume only in MS (p < 0.05). Significant correlations between diffusion alterations, several subfield volumes and clinical variables were observed in both diseases, especially in MS (R = -0.444 to 0.498, p < 0.05). FA and MD showed fair discriminative power between MS and HC, NMOSD and HC (AUC > 0.7). Conclusions: Hippocampal atrophy and diffusion abnormalities were identified in MS and NMOSD, partly explaining how clinical disability and cognitive impairment are differentially affected.
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| 6. |
- Zhou, Yunyun, et al.
(författare)
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Anti-Kasha's Rule Emissive Switching Induced by Intermolecular H-Bonding
- 2018
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Ingår i: Chemistry of Materials. - : AMER CHEMICAL SOC. - 0897-4756 .- 1520-5002. ; 30:21, s. 8008-8016
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Tidskriftsartikel (refereegranskat)abstract
- The exploration of emission pathways from high-excited states in organic luminogens has recently become prosperous owing to improved possibilities to study so-called anti-Kasha's rule emission with the potential of improving the luminescent quantum efficiency. However, emission pathway switching among different high-excited states has rarely been addressed through external control. We here present a rational design and synthesis of a novel azulene-based emitter to achieve a responsive control of its anti-Kasha's rule emissive switching. The emitter initially gives rise to an S-3-to-S-0 dominant emission as indicated by our experimental and theoretical studies. On this basis, it can be toggled into an S-2-to-S-0 dominant emission upon the H-bond formation between the triformyl groups and water molecules. Such a process, which originates from the H-bonding regulated distribution of excited state energy, is accompanied by a remarkable fluorescent color conversion and a significant improvement of the fluorescent quantum yield in the azulene family. Moreover, a reversible emissive switching in doped films was observed to depend on a solid-state H-bond tuning process with moisture sensitivity. These results may provide new insight for building advanced chemical systems for visualized sensing with high distinguishability.
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| 7. |
- Zhou, Yunyun, et al.
(författare)
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Selective Dual-Channel Imaging on Cyanostyryl-Modified Azulene Systems with Unimolecularly Tunable Visible-Near Infrared Luminescence
- 2017
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Ingår i: Chemistry - A European Journal. - : Wiley-VCH Verlagsgesellschaft. - 0947-6539 .- 1521-3765. ; 23:32, s. 7642-7647
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Tidskriftsartikel (refereegranskat)abstract
- Although organic light-emitting molecules have received a growing attention and applicability in modern bioimaging science, the design and control of complex photoluminescent properties in unimolecularly selective imaging remains a challenging topic. Considering that tunable multipathway imaging can be advantagedly connected with treatment processes in therapy, the integration of an azulene and a cyanostyryl moiety into one skeleton is carried out for the generation of in situ stimuli-responsive luminescent materials, with the aim to achieve tunable and effective emissions in distinct channels through smart molecular design on a single-molecular platform. This strategy takes advantage of 1) the Z/E isomerization of the cyanostyryl unit that can vary the push-pull effect of the substitution on azulene, accompanied by altering absorption and emission of individual excited states, and 2) an optimized excited-state regulation for opening a near infrared emissive channel and making up for a controllable dual-pathway luminescent system together with the utilization of visible emission. As exemplified by a demonstration of manipulating the luminescence at the cell level, the materials exhibit a superior application potential for unimolecularly selective imaging, labeling and probing events.
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